Combined application of 13C NMR spectroscopy and confocal laser scanning microscopy—Investigation on biofilm structure and physico-chemical properties

Garny, K.; Neu, Thomas R.; Horn, Harald; Volke, F.; Manz, B.

doi:10.1016/j.ces.2010.05.013

Cited by 20 publications

(14 citation statements)

References 41 publications

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“…A recent report on the response of heterotrophic, mixed‐species biofilms cultivated in rotating annular reactors to carbon starvation mirrored the results reported here. Biofilm biomass, thickness and the rate of aggregate detachment decreased, along with a change in the volumetric ratio of cells to EPS in the remaining attached biomass (Garny et al. 2010).…”

Section: Discussionmentioning

confidence: 99%

Biofilm form and function: carbon availability affects biofilm architecture, metabolic activity and planktonic cell yield

Bester¹,

Kroukamp²,

Hausner³

et al. 2010

Journal of Applied Microbiology

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Aims: To investigate carbon transformation by biofilms and changes in biofilm architecture, metabolic activity and planktonic cell yield in response to fluctuating carbon availability. Methods and Results: Pseudomonas sp. biofilms were cultured under alternating carbon‐replete and carbon‐limited conditions. A shift to medium without added carbon led to a 90% decrease in biofilm respiration rate and a 40% reduction in planktonic cell yield within 1 h. Attached cell division and progeny release were shown to contribute to planktonic cell numbers during carbon limitation. Development of a significantly enlarged biofilm surface area during carbon limitation facilitated a rapid increase in whole‐biofilm metabolic activity, cell yield and biomass upon the re‐introduction of carbon after 8 days of limitation. The cumulative number of planktonic cells (>1010 CFU) released from the biofilm during the cultivation period contained only 1·0% of the total carbon available to the biofilm, with 6·5% of the carbon retained in the biofilm and 54% mineralized to CO2. Conclusions: Biofilm‐derived planktonic cell yield is a proliferation mechanism. The rapid response of biofilms to environmental perturbations facilitates the optimal utilization of resources to promote both proliferation and survival. Biofilms function as efficient catalysts for environmental carbon transformation and mineralization. Significance and Impact of the study: A greater understanding of the relationship between biofilm form and function can inform strategies intended to control and/or promote biofilm formation.

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Section: Discussionmentioning

confidence: 99%

Biofilm form and function: carbon availability affects biofilm architecture, metabolic activity and planktonic cell yield

Bester¹,

Kroukamp²,

Hausner³

et al. 2010

Journal of Applied Microbiology

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“…The molecular mixing model iteratively determines the linear combination of six major SOC constituents (BC, carbohydrate, lignin, lipid, protein, and carbonyl) that best fit the integrated regions of the NMR spectra [ Baldock et al , 2004]. For example, aromatic carbon in general contributes to the 13 C NMR aromatic peak intensity at 130 ppm (Figure S1 in the auxiliary material), but condensed aromatic carbon atoms in BC can be separated from single aromatic rings present in biological molecules (lignin or protein) because the latter also contribute to spectral intensity at 56 and 150 ppm [ Garny et al , 2010; Krull et al , 2006]. Tannins are not a quantitatively important component of the carbon pool [ Adamczyk et al , 2009; Hernes and Hedges , 2000], and therefore, are not considered in the mixing model.…”

Section: Methodsmentioning

confidence: 99%

Mineralogical controls on soil black carbon preservation

Cusack

Chadwick

Hockaday

et al. 2012

Global Biogeochemical Cycles

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[1] Black carbon (BC) has long been considered a chemically resistant component of soil organic carbon (SOC). However, there is substantial evidence that the chemistry of most C compounds is less important for long-term storage than is physical protection (e.g., mineral sorption). We explored BC retention in grasslands that lie along a climate gradient that produces strong differences in short range order (SRO) minerals known to drive landscape-scale retention of SOC. We measured soil BC content using 13 C nuclear magnetic resonance (NMR) spectroscopy, and used radiocarbon dating on a subset of samples to relate BC content to long-term soil C storage. Black C concentrations in soil ranged from 0.2 to 2.9%, representing 10-30% of SOC and spanning levels found in temperate grasslands around the world. Black C concentrations were significantly correlated with SRO minerals, but the strongest single predictor of BC content was simply SOC. The ratio of BC/OC was fairly insensitive to SRO minerals, suggesting that BC responds similarly to reactive minerals as does OC. Direct links between SOC and BC retention warrant further study. We found no evidence that BC is preferentially retained relative to OC, with soil radiocarbon ages apparently driven primarily by inputs of new OC. These results indicate that physical protection may play a strong role in BC retention, and that BC cycling in soils may be more similar to OC cycling than is generally thought.

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“…Structural changes in bacteria biofilms have been well characterized experimentally under several growth conditions, and it has been shown that the architecture is strongly species and strain-dependent [10,11,12,13]. From a compositional point of view, it has been shown that changes of structural parameters in bacterial biofilms are strongly correlated to the biochemical composition of exopolymers [14,15,16,17]. Phototrophic biofilms, especially microalgae biofilms, have been far less studied [8,18,19,20,21,22].…”

Section: Introductionmentioning

confidence: 99%

The Architecture of Monospecific Microalgae Biofilms

et al. 2019

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Microalgae biofilms have been proposed as an alternative to suspended cultures in commercial and biotechnological fields. However, little is known about their architecture that may strongly impact biofilm behavior, bioprocess stability, and productivity. In order to unravel the architecture of microalgae biofilms, four species of commercial interest were cultivated in microplates and characterized using a combination of confocal laser scanning microscopy and FTIR spectroscopy. In all the species, the biofilm biovolume and thickness increased over time and reached a plateau after seven days; however, the final biomass reached was very different. The roughness decreased during maturation, reflecting cell division and voids filling. The extracellular polymeric substances content of the matrix remained constant in some species, and increased over time in some others. Vertical profiles showed that young biofilms presented a maximum cell density at 20 μm above the substratum co-localized with matrix components. In mature biofilms, the maximum density of cells moved at a greater distance from the substratum (30–40 μm), whereas the maximum coverage of matrix components remained in a deeper layer. Carbohydrates and lipids were the main macromolecules changing during biofilm maturation. Our results revealed that the architecture of microalgae biofilms is species-specific. However, time similarly affects the structural and biochemical parameters.

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Combined application of 13C NMR spectroscopy and confocal laser scanning microscopy—Investigation on biofilm structure and physico-chemical properties

Cited by 20 publications

References 41 publications

Biofilm form and function: carbon availability affects biofilm architecture, metabolic activity and planktonic cell yield

Biofilm form and function: carbon availability affects biofilm architecture, metabolic activity and planktonic cell yield

Mineralogical controls on soil black carbon preservation

The Architecture of Monospecific Microalgae Biofilms

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