Combined application of bacterial predation and carbon dioxide aerosols to effectively remove biofilms

Dwidar, Mohammed; Hong, Seongkyeol; Cha, Minju; Jang, Jaesung; Mitchell, Robert J.

doi:10.1080/08927014.2012.701286

Cited by 27 publications

(22 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The majority of gastrointestinal infections and other bacterial infections in general involve biofilm formation by the invading pathogen (Wood et al ., ). Furthermore, previous studies demonstrated that B. bacteriovorus can effectively attack bacterial biofilms and reduce their populations (Fratamico and Cooke, ; Kadouri and O'Toole, ; Dwidar et al ., ). Indole is known to affect E. coli biofilm formation (Lee et al ., ) and, at the same time, our results found that it blocks predation in liquid cultures.…”

Section: Discussionmentioning

confidence: 97%

Indole negatively impacts predation by Bdellovibrio bacteriovorus and its release from the bdelloplast

Dwidar

Nam

Mitchell

2014

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

Bdellovibrio bacteriovorus is a predatory bacterium that attacks a wide range of Gram-negative bacterial pathogens and is proposed to be a potential living antibiotic. In this study, we evaluated the effects of indole, a bacterial signalling molecule commonly produced within the gut, on the predatory ability of B. bacteriovorus HD100. Indole significantly delayed predation on Escherichia coli MG1655 and Salmonella enterica KACC 11595 at physiological concentrations (0.25 to 1 mM) and completely inhibited predation when present at 2 mM. Microscopic analysis revealed that indole blocked the predator from attacking the prey. Furthermore, indole was not toxic to the predator but slowed down its motility. Microarray and reverse transcription quantitative polymerase chain reaction (RT-qPCR) analyses confirmed that as the gene group showing the greatest downregulation in the presence of indole was flagellar assembly genes. Indole also caused a wide spectrum changes in gene expression including general downregulation of genes involved in ribosome assembly. Furthermore, indole addition to the predatory culture after the entrance of B. bacteriovorus into the prey periplasm slowed down bdelloplast lysis. In conclusion, indole can have significant impacts on the predation efficiency, which should be taken into consideration especially if B. bacteriovorus is to be applied as a probiotic or living antibiotic.

show abstract

Section: Discussionmentioning

confidence: 97%

Indole negatively impacts predation by Bdellovibrio bacteriovorus and its release from the bdelloplast

Dwidar

Nam

Mitchell

2014

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Confocal microscopy was performed using an LSM700 confocal microscope (Carl Zeiss) operated by ZEN 2009 software. Scanning electron microscopy imaging of these biofilms was performed as described previously10. The images shown are representatives for the results obtained from three different chips that were prepared and analysed independently for each case.…”

Section: Methodsmentioning

confidence: 99%

“…B. bacteriovorus was also shown to be a very promising tool for combating biofilms101112. This was attributed to the ability of this bacterium to penetrate deeply inside prey biofilms and effectively destroy them; a characteristic which distinguishes them from other biological tools such as bacteriophages and protists12.…”

mentioning

confidence: 99%

“…This was attributed to the ability of this bacterium to penetrate deeply inside prey biofilms and effectively destroy them; a characteristic which distinguishes them from other biological tools such as bacteriophages and protists12. A major limitation of BALOs, however, is their inability to attack or predate upon Gram-positive strains81013, a category that comprises numerous human pathogens1415, including Staphylococcus aureus , one of the most frequent nosocomial infection-associated multidrug resistant pathogens isolated from patients1617.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Bdellovibrio bacteriovorus Inhibits Staphylococcus aureus Biofilm Formation and Invasion into Human Epithelial Cells

Monnappa

Dwidar

Seo

et al. 2014

Sci Rep

Self Cite

View full text Add to dashboard Cite

Bdellovibrio bacteriovorus HD100 is a predatory bacterium that attacks many Gram-negative human pathogens. A serious drawback of this strain, however, is its ineffectiveness against Gram-positive strains, such as the human pathogen Staphylococcus aureus. Here we demonstrate that the extracellular proteases produced by a host-independent B. bacteriovorus (HIB) effectively degrade/inhibit the formation of S. aureus biofilms and reduce its virulence. A 10% addition of HIB supernatant caused a 75% or greater reduction in S. aureus biofilm formation as well as 75% dispersal of pre-formed biofilms. LC-MS-MS analyses identified various B. bacteriovorus proteases within the supernatant, including the serine proteases Bd2269 and Bd2321. Tests with AEBSF confirmed that serine proteases were active in the supernatant and that they impacted S. aureus biofilm formation. The supernatant also possessed a slight DNAse activity. Furthermore, treatment of planktonic S. aureus with the supernatant diminished its ability to invade MCF-10a epithelial cells by 5-fold but did not affect the MCF-10a viability. In conclusion, this study illustrates the hitherto unknown ability of B. bacteriovorus to disperse Gram-positive pathogenic biofilms and mitigate their virulence.

show abstract

“…They typically have a broad prey range but are unable to attack Gram‐positive or eukaryotic cells in co‐cultures, making them and/or their lytic enzymes candidates for use as biological antimicrobials against Gram‐negative bacterial pathogens (Sockett and Lambert, ; Markelova, ; Dashiff and Kadouri, ; Kadouri et al ., ). The ability of Bdellovibrio bacteriovorus to disrupt biofilms formed by Gram‐negative bacteria (Kadouri and O'Toole, ; Van Essche et al ., ; Dwidar et al ., ; Kadouri and Tran, ; Chanyi and Koval, ) adds to the potential therapeutic use of BALOs. Interestingly, a recent study by Monnappa et al .…”

Section: Introductionmentioning

confidence: 99%

Stenotrophomonas maltophilia biofilm reduction by Bdellovibrio exovorus

Chanyi

Koval

Brooke

2016

Environ Microbiol Rep

View full text Add to dashboard Cite

Stenotrophomonas maltophilia, a bacterium ubiquitous in the environment, is also an opportunistic, multidrug-resistant human pathogen that colonizes tissues and medical devices via biofilm formation. We investigated the ability of an isolate from sewage of the bacterial predator Bdellovibrio exovorus to disrupt preformed biofilms of 18 strains of S. maltophilia isolated from patients, hospital sink drains and water fountain drains. B. exovorus FFRS-5 preyed on all S. maltophilia strains in liquid co-cultures and was able to significantly disrupt the biofilms of 15 of the S. maltophilia strains tested, decreasing as much as 76.7% of the biofilm mass. The addition of ciprofloxacin and kanamycin in general reduced S. maltophilia biofilms but less than that of B. exovorus alone. Furthermore, when antibiotics and B. exovorus were used together, B. exovorus was still effective in the presence of ciprofloxacin whereas the addition of kanamycin reduced the effectiveness of B. exovorus. Overall, B. exovorus was able to decrease the mass of preformed biofilms of S. maltophilia in the presence of clinically relevant antibiotics demonstrating that the predator may prove to be a beneficial tool to reduce S. maltophilia environmental or clinically associated biofilms.

show abstract

Combined application of bacterial predation and carbon dioxide aerosols to effectively remove biofilms

Cited by 27 publications

References 38 publications

Indole negatively impacts predation by Bdellovibrio bacteriovorus and its release from the bdelloplast

Indole negatively impacts predation by Bdellovibrio bacteriovorus and its release from the bdelloplast

Bdellovibrio bacteriovorus Inhibits Staphylococcus aureus Biofilm Formation and Invasion into Human Epithelial Cells

Stenotrophomonas maltophilia biofilm reduction by Bdellovibrio exovorus

Contact Info

Product

Resources

About