Combined assessment of cyto-, geno-, and embryotoxicity of silica nanoparticles in experiments in vitro

Protasova, G. A.; Popov, V. B.; Shabasheva, L. V.; Strekalovskii, I. V.; Panferova, Y. A.; Khlebnikova, Nadezhda; Radilov, A. A.

doi:10.1088/1742-6596/838/1/012029

Cited by 1 publication

(1 citation statement)

References 13 publications

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“…Our present results on BEAS-2B cells exposed to titania MNMs, did not show any micronuclei induction confirming previous data on other cellular models (Andreoli et al, 2018;Zijno et al, 2015). Also, the two silica MNMs did not induce any MN increase confirming other in vitro studies on different cell types (Guichard et al, 2016;Protasova et al, 2017). On the other hand, we found slight direct and oxidative DNA damage for both titania and NM203 by Fpg-Comet assay.…”

Section: Discussionsupporting

confidence: 91%

Use of a common European approach for nanomaterials’ testing to support regulation: a case study on titanium and silicon dioxide representative nanomaterials

Zijno

Cavallo

Felice

et al. 2020

J of Applied Toxicology

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The European Union (EU) continuously takes ensuring the safe use of manufactured nanomaterials (MNMs) in consumer products into consideration. The application of a common approach for testing MNMs, including the use of optimized protocols and methods' selection, becomes increasingly important to obtain reliable and comparable results supporting the regulatory framework. In the present study, we tested four representative MNMs, two titanium dioxides (NM100 and NM101) and two silicon dioxides (NM200 and NM203), using the EU FP7-NANoREG approach, starting from suspension and dispersion preparations, through to their characterization and final evaluation of biological effects. MNM dispersions were prepared following a refined NANOGENOTOX protocol and characterized by dynamic light scattering (DLS) in water/bovine serum albumin and in media used for in vitro testing. Potential genotoxic effects were evaluated on human bronchial BEAS-2B cells using micronucleus and Comet assays, and pro-inflammatory effects by cytokines release. Murine macrophages RAW 264.7 were used to detect potential innate immune responses using two functional endpoints (pro-inflammatory cytokines and nitric oxide [NO] production). The interaction of MNMs with RAW 264.7 cells was studied by electron microscopy. No chromosomal damage and slight DNA damage and an oxidative effect, depending on MNMs, were observed in bronchial cells. In murine macrophages, the four MNMs directly induced tumor necrosis factor α or interleukin 6 secretion, although at very low levels; lipopolysaccharide-induced NO production was significantly decreased by the titania and one silica MNM. The application of this approach for the evaluation of MNM biological effects could be useful for both regulators and industries.

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