Combined chemoenzymatic strategy for sustainable continuous synthesis of the natural product hordenine

Gianolio, Stefania; Padrosa, David Roura; Paradisi, Francesca

doi:10.1039/d2gc02767d

Cited by 12 publications

(8 citation statements)

References 49 publications

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“…Harmol hydrochloride was found in NTJ might be due to the reason that hydrochloride form of harmol increased its solubility, thus it existed in NTJ. It has been reported that under the catalysis of tyrosine decarboxylase from Lactobacillus brevis, tyrosine can be converted to hordenine [26]. Tyrosine was detected in NJF prior to fermentation, and vanished after fermenation, (Table 2), and lactic acid bacteria were employed in this fermentation process, therefore, it is very likely that during the fermentation tyrosine was converted to hordenine.…”

Section: Fermentation Modified Bioactive Compounds In Ntf and Altered...mentioning

confidence: 96%

Combined Microbial Fermentation Converts Bioactive Compounds in Nitraria Tangutorum Bobrov Fruit and Displays its Antidiabetic Potential

Yang,

Liub

2024

JSM Biotechnol Bioeng

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Nitraria tangutorum Bobrov is a berry shrub with white flowers and red fruits, which grows in the deserts in the Tibetan Plateau, Mongolia, and western China. Its fruit N. tangutorum Fruit (NTF) contains various bioactive compounds, with anti-fatigue, anti-inflammatory, and neuroprotective functions. However, the high saccharide content of NTF makes it unsuitable for diabetic patients. In this study, we fermented NTF to obtain N. tangutorum Fermented Juice (NTJ), and N. tangutorum Fermented Residue (NTR), which are suitable for diabetics to consume. We characterized the bioactive compounds in NTF, NTR, and NTJ, and found that fermentation increased the diversity of bioactive compounds, and greatly reduced sucrose, glucose, and fructose content while generating trehalose, which has the potential to manage blood glucose levels. Further, NTJ displays anti-diabetic potential due to various compounds anti-diabetic properties. This study provides a basis for further clinical research on NTJ’s anti-diabetic function in humans.

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Section: Fermentation Modified Bioactive Compounds In Ntf and Altered...mentioning

confidence: 96%

Combined Microbial Fermentation Converts Bioactive Compounds in Nitraria Tangutorum Bobrov Fruit and Displays its Antidiabetic Potential

Yang,

Liub

2024

JSM Biotechnol Bioeng

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show abstract

“…It has been reported that under the catalysis of tyrosine decarboxylase from Lactobacillus brevis, tyrosine can be converted to hordenine. [26] Tyrosine was detected in NJF prior to fermentation, and vanished after fermenation, (see Table 2), and lactic acid bacteria were employed in this fermentation process, therefore, it is very likely that during the fermentation tyrosine was converted to hordenine.…”

Section: Fermentation Modified Bioactive Compounds In Ntf and Altered...mentioning

confidence: 99%

Combined Microbial Fermentation Converts Bioactive Compounds in Nitraria tangutorum Bobrov Fruit and Displays Its Antidiabetic Potential

Yang,

Liu

2024

Preprint

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Nitraria tangutorum Bobrov is a berry shrub with white flowers and red fruits, which grows in the deserts in the Tibetan Plateau, Mongolia, and western China. Its fruit N. tangutorum fruit (NTF) contains various bioactive compounds, with anti-fatigue, anti-inflammatory, and neuroprotective functions. However, the high saccharide content of NTF makes it unsuitable for diabetic patients. In this study, we fermented NTF to obtain N. tangutorum fermented juice (NTJ), and N. tangutorum fermented residue (NTR), which are suitable for diabetics to consume. We characterized the bioactive compounds in NTF, NTR, and NTJ, and found that fermentation increased the diversity of bioactive compounds, and greatly reduced sucrose, glucose, and fructose content while generating trehalose, which has the potential to manage blood glucose levels. Further, NTJ displays anti-diabetic potential due to various compounds anti-diabetic properties. This study provides a basis for further clinical research on NTJ's anti-diabetic function in humans.

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“…Enzyme-catalyzed reactions offer highly specific and efficient routes to the synthesis of a wide range of valuable chemicals. − Greater use of enzymatic biocatalysis in industry is, however, limited by several factors such as feedback inhibition and limited processing conditions including temperatures and solvents and long-term instability of the biocatalysts . While the reaction rate can be enhanced through the use of cofactors, in many cases, these are consumed in the reaction and are fugitive and expensive.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Model for the Heterogeneous Biocatalytic Reactions Using Tethered Cofactors

McDonough,

Williams,

Hartley

et al. 2024

Langmuir

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Understanding the mechanism of interfacial enzyme kinetics is critical to the development of synthetic biological systems for the production of value-added chemicals. Here, the interfacial kinetics of the catalysis of β-nicotinamide adenine dinucleotide (NAD + )-dependent enzymes acting on NAD + tethered to the surface of silica nanoparticles (SiNPs) has been investigated using two complementary and supporting kinetic approaches: enzyme excess and reactant (NAD + ) excess. Kinetic models developed for these two approaches characterize several critical reaction steps including reversible enzyme adsorption, complexation, decomplexation, and catalysis of the surface-bound enzyme/NAD + complex. The analysis reveals a concentrating effect resulting in a very high local concentration of enzyme and cofactor on the particle surface, in which the enzyme is saturated by surface-bound NAD, facilitating a rate enhancement of enzyme/ NAD + complexation and catalysis. This resulted in high enzyme efficiency within the tethered NAD + system compared to that of the free enzyme/NAD + system, which increases with decreasing enzyme concentration. The role of enzyme adsorption onto solid substrates with a tethered catalyst (such as NAD + ) has potential for creating highly efficient flow biocatalytic systems.

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Combined chemoenzymatic strategy for sustainable continuous synthesis of the natural product hordenine

Abstract: To improve sustainability, safety and cost-efficiency of synthetic methodologies, biocatalysis can be a helpful ally. In this work, a novel chemoenzymatic stategy ensures the rapid synthesis of hordenine, a valuable...

Cited by 12 publications

References 49 publications

Combined Microbial Fermentation Converts Bioactive Compounds in Nitraria Tangutorum Bobrov Fruit and Displays its Antidiabetic Potential

Combined Microbial Fermentation Converts Bioactive Compounds in Nitraria Tangutorum Bobrov Fruit and Displays its Antidiabetic Potential

Combined Microbial Fermentation Converts Bioactive Compounds in Nitraria tangutorum Bobrov Fruit and Displays Its Antidiabetic Potential

Kinetic Model for the Heterogeneous Biocatalytic Reactions Using Tethered Cofactors

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