COMBINED CHIP APPLICATION'S EFFECT OF CLINICAL PARAMETERS AND TNF-α EXPRESSIONS

Abstract

Objective: Scaling root planing is the main mechanical treatment to remove local deposits. Supportive local and systemic antimicrobial therapy is used to eliminate or reduce the number of pathogenic bacteria. The chip application of the combination of snakehead fish extract and betel leaf can change the clinical parameters for the better and increase the expression of TNF-α as a pro-inflammatory. To see the effect of the application of a chip combination of snakehead fish extract and betel leaf in male Wistar rats induced by periodontitis as a support for initial therapy on clinical parameters and expression of TNF-α. Methods: Laboratory experimental study with a posttest control group design in which 24 male Wistar rats induced periodontitis were in four treatment groups (2.5%, 5%, 10% snakehead fish extract and placebo). Examination of clinical parameters and expression of TNF-α was carried out on day 0, day 3 and day 7. Data were analyzed by the Mann-Whitney test. Results: The results showed that the clinical parameters; gingival index, pocket depth and attachment level looked better on the 7th day after treatment with statistically significant results (p≤0.05) and the highest anti-inflammatory TNF-α expression occurred at a concentration of 5%. Conclusion: The application of a chip combination of snakehead fish extract and betel leaf as a support for initial therapy is effective in reducing the gingival index pocket depth and increasing the level of attachment, which is statistically significant, but there is no significant difference in TNF-α expression.