Combined delivery of FGF‐2, TGF‐β1, and adipose‐derived stem cells from an engineered periosteum to a critical‐sized mouse femur defect

Romero, Raimundo; Travers, John K.; Asbury, Emilie; Pennybaker, Attie; Chubb, Laura S.; Rose, R.; Ehrhart, Nicole; Kipper, Matt J.

doi:10.1002/jbm.a.35965

Cited by 35 publications

(30 citation statements)

References 91 publications

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“…Nevertheless, their application is still limited by the amount of available healthy periosteum . Many studies that used biotic and abiotic materials that mimic periosteum have achieved progress …”

Section: Introductionmentioning

confidence: 99%

Decellularized Periosteum‐Covered Chitosan Globule Composite for Bone Regeneration in Rabbit Femur Condyle Bone Defects

Pang

Zeng

et al. 2018

Macromolecular Bioscience

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Critical-sized bone defects are incapable of self-healing and are commonly seen in clinical practice. The authors explore a new treatment for this, decellularized periosteum is applied to chitosan globules (chitosan-DP globules) as a hybrid material. The efficacy of chitosan-DP globules on rabbit femoral condyle bone defects is assessed with biocompatibility, biomechanics, and osteogenic efficiency measurements, and compared with the results of chitosan globules and empty control. No difference in cytotoxicity is observed among chitosan-DP globules, chitosan globules, and the empty control. Chitosan-DP globules possesse a better surface for cell adhesion than did chitosan globules. Chitosan-DP globules demonstrate superior efficiency for osteogenesis in the defect area compared to chitosan globules as per microcomputed tomography examination and push-out testing, with relatively minor histological differences. Both chitosan globule groups show more satisfactory results than those for the empty control. The results implicate chitosan-DP globules as a promising solution for bone defects.

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Section: Introductionmentioning

confidence: 99%

Decellularized Periosteum‐Covered Chitosan Globule Composite for Bone Regeneration in Rabbit Femur Condyle Bone Defects

Pang

Zeng

et al. 2018

Macromolecular Bioscience

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“…Granero-Molto et al and Undale et al reported that transplanted MSCs enhanced callus volume, increased new bone volume, and improved biomechanical properties and could induce fracture healing by increasing biomechanical properties in mice and non-union nude rat model (52). Romero et al also described the potential of growth factor and MSCs to stimulate the healing process and new bone formation in a critical-sized mouse femur defect without inflammatory response to the graft (53). The outcome of our study is in agreement with the above-mentioned researches that declare PSC seeded in bone allograft enriched by human growth factors, which could accelerate the formation of bone callus at the fracture gap in rabbit radial CSD.…”

Section: Discussionmentioning

confidence: 99%

Allogenic Bone Graft Enriched by Periosteal Stem Cell and Growth Factors for Osteogenesis in Critical Size Bone Defect in Rabbit Model: Histopathological and Radiological Evaluation

et al. 2020

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Scan to discover online Background & Objective: This study aimed to investigate the effect of decellularized allogeneic bone graft enriched by periosteal stem cells (PSCs) and growth factors on the bone repair process in a rabbit model, which could be used in many orthopedic procedures. Methods: In this experimental study, a critical size defect (CSD) (10 mm) was created in the radial diaphysis of 40 rabbits. In group A, the defect was left intact with no medical intervention. In group B, the defect was filled by a decellularized bone graft. In group C, the defect was implanted by a decellularized bone graft enriched with platelet growth factors. In group D, the defect was treated by a decellularized bone graft seeded by periosteal mesenchymal stem cells (MSCs). Also, in group E, the defect was filled by a decellularized bone graft enriched with platelet growth factors and periosteal MSCs. Radiological evaluation was done on the first day and then in the second, fourth, and eighth weeks after the operation. The specimens were harvested on the 28th and 56th postoperative days and evaluated for histopathological criteria. Results: The radiologic and microscopic analysis of the healing process in bone defects of the treated groups (C, D, and E) revealed more advanced repair criteria than those of groups A and B significantly (P<0.05). Conclusion: Based on this study, it appears that implantation of concentrated PSCs in combination with growth factors and allogeneic cortical bone graft is an effective therapy for the repair of large bone defects.

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“…Polysaccharide based tissue engineered periosteum composed of heparin-coated chitosan nanofibers was studied in terms of delivering growth factors, support adiposederived mesenchymal stem cells (ASCs) delivery and improvement of the incorporation of the allograft in a critical-sized mouse femoral defect [144]. Female mouse received untreated allografts, allografts seeded with ASCs, allografts modified with nanofibers, or allografts modified with nanofibers and ASCs.…”

Section: Polymer Biomaterialsmentioning

confidence: 99%

Scaffolds and coatings for bone regeneration

Pereira

Cengiz

Silva

et al. 2020

J Mater Sci: Mater Med

109

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Bone tissue has an astonishing self-healing capacity yet only for non-critical size defects (<6 mm) and clinical intervention is needed for critical-size defects and beyond that along with non-union bone fractures and bone defects larger than critical size represent a major healthcare problem. Autografts are, still, being used as preferred to treat large bone defects. Mostly, due to the presence of living differentiated and progenitor cells, its osteogenic, osteoinductive and osteoconductive properties that allow osteogenesis, vascularization, and provide structural support. Bone tissue engineering strategies have been proposed to overcome the limited supply of grafts. Complete and successful bone regeneration can be influenced by several factors namely: the age of the patient, health, gender and is expected that the ideal scaffold for bone regeneration combines factors such as bioactivity and osteoinductivity. The commercially available products have as their main function the replacement of bone. Moreover, scaffolds still present limitations including poor osteointegration and limited vascularization. The introduction of pores in scaffolds are being used to promote the osteointegration as it allows cell and vessel infiltration. Moreover, combinations with growth factors or coatings have been explored as they can improve the osteoconductive and osteoinductive properties of the scaffold. This review focuses on the bone defects treatments and on the research of scaffolds for bone regeneration. Moreover, it summarizes the latest progress in the development of coatings used in bone tissue engineering. Despite the interesting advances which include the development of hybrid scaffolds, there are still important challenges that need to be addressed in order to fasten translation of scaffolds into the clinical scenario. Finally, we must reflect on the main challenges for bone tissue regeneration. There is a need to achieve a proper mechanical properties to bear the load of movements; have a scaffolds with a structure that fit the bone anatomy. Graphical Abstract* Helena Filipa Pereira

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Combined delivery of FGF‐2, TGF‐β1, and adipose‐derived stem cells from an engineered periosteum to a critical‐sized mouse femur defect

Cited by 35 publications

References 91 publications

Decellularized Periosteum‐Covered Chitosan Globule Composite for Bone Regeneration in Rabbit Femur Condyle Bone Defects

Decellularized Periosteum‐Covered Chitosan Globule Composite for Bone Regeneration in Rabbit Femur Condyle Bone Defects

Allogenic Bone Graft Enriched by Periosteal Stem Cell and Growth Factors for Osteogenesis in Critical Size Bone Defect in Rabbit Model: Histopathological and Radiological Evaluation

Scaffolds and coatings for bone regeneration

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