Combined Determination of the Small Intestine Walls Luminescence Intensity to Assess Its Viability

Abstract

Resume. Aim: to study the informativeness of the photoluminescence intensity (PLI) indicators of the small intestine walls to assess its viability. Material and methods. 30 white rats with small bowel obstruction models in the initial (10 rats), middle (10 rats), terminal parts. In 6 h, the intestines were irradiated with a laser beam (λ=458 nm). After measuring PLI, intestinal tissues were taken for histological examination. The examination data of 10 intact rats were the control. Results. PLI is significantly decreases if viability is disrupted. Parameters of absolute indicators have significant local and individual variability. Using the ratio of PLI parameters at wavelengths λ=474 and λ=489 nm eliminates variability. The parameters of the ratio differ significantly, depending on the degree of viability disruption. Conclusions. 1. Viability disruption of the small intestine are associated with a significant decrease in the ratio parameters of the its walls luminescence intensity indicators at the wavelengths λ=474 and λ=489 nm. Ratio parameters > 20 un mark preserved viability, parameters within 17-13 un mark morphological disorders without necrosis, parameters < 12 un mark necrotic disorders.