Combined effect of cadmium, benzo(a)pyrene and pyrene on the Wistar male rats reproductive system

Paltanaviciene, A.; Zabulytė, D.; Kalibatas, Julius

doi:10.5414/tep23251

“…After a 7-day acclimation period, the rats were allocated into equal experimental groups. They were administered either a dose of 50 mg/kg/BW (equivalent to 1/20 of LD50, as per Paltanaviciene et al (2007) of B[a]P dissolved in 10 ml/kg/BW of corn oil through oral gavage twice weekly or a vehicle consisting of an equivalent volume of corn oil, as outlined by Dhatwalia et al (2019).…”

Section: Induction Of B[a]p-intoxicationmentioning

confidence: 99%

The Gap and Food Security of Poultry Meat in the Kingdom of Saudi Arabia

M.F. Al Harajin,

S. Shehata,

M.A. Abdel Moneim

2024

Alexandria Science Exchange Journal

0

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Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, poses a significant threat to human health as an increasingly prevalent environmental and foodborne pollutant. Our previous study revealed that wild Silybum marianum seeds ethanolic extract (SMSE) attenuated B[a]P-induced oxidative stress and cell apoptosis in albino rats. In this work, we extended our hypothesis to investigate the potential protective effects of SMSE against hepatic and nephritic injuries induced by B[a]P. Thirty-six male albino rats were assigned into six groups: Group I, the control (-Ve), received 10 ml/kg/BW corn oil orally twice weekly; Group II, the control (+Ve), was given 50 mg/kg/BW of B[a]P in corn oil twice weekly; and Groups III, V, IV, and VI were orally administered B[a]P + SMSE at 200, 400, 600, and 800 mg/kg BW/day, respectively, for four weeks. B[a]P exposure significantly (p≤0.05) increased the relative weights of the liver (33.33%) and kidney (39.68%); cytochrome P450 (CYP450) activity, a biotransformation marker, (68.25%); tumor necrosis factor-alpha (TNF-α) levels, a pro-inflammatory mediator, (95.95%); liver enzymes activities (AST, 124.09%, ALT, 124.98%, and ALP, 128.77%); and renal function indicators (serum urea, 60.18%, and creatinine, 68.35%), accompanied by severe histopathologic changes in liver and kidney tissues compared to control (-Ve). Conversely, SMSE interventions significantly (p≤0.05) dosedependently reduced elevated rates of these indices, while improving liver and kidney histoarchitecture. The SMSE's protective effects may result from its bioactive compounds content that regulate B[a]P metabolic enzymes, inhibit CYP450 activity, suppress TNF-α, and scavenge other reactive intermediates. Our results suggest SMSE as a potential natural approach to alleviating environmental pollutants' detrimental effects and deserves further research.

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“…After a 7-day acclimation period, the rats were allocated into equal experimental groups. They were administered either a dose of 50 mg/kg/BW (equivalent to 1/20 of LD50, as per Paltanaviciene et al (2007) of B[a]P dissolved in 10 ml/kg/BW of corn oil through oral gavage twice weekly or a vehicle consisting of an equivalent volume of corn oil, as outlined by Dhatwalia et al (2019).…”

Section: Induction Of B[a]p-intoxicationmentioning

confidence: 99%

Potential Protective Effects of Milk Thistle (Silybum Marianum L.) Seeds Against Benzo[a]Pyrene-Induced Hepatic and Nephritic Injuries in Rats: Biochemical and Histopathological Studies.

Elhassaneen,

Mahran

2024

Alexandria Science Exchange Journal

0

View full text Add to dashboard Cite

Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, poses a significant threat to human health as an increasingly prevalent environmental and foodborne pollutant. Our previous study revealed that wild Silybum marianum seeds ethanolic extract (SMSE) attenuated B[a]P-induced oxidative stress and cell apoptosis in albino rats. In this work, we extended our hypothesis to investigate the potential protective effects of SMSE against hepatic and nephritic injuries induced by B[a]P. Thirty-six male albino rats were assigned into six groups: Group I, the control (-Ve), received 10 ml/kg/BW corn oil orally twice weekly; Group II, the control (+Ve), was given 50 mg/kg/BW of B[a]P in corn oil twice weekly; and Groups III, V, IV, and VI were orally administered B[a]P + SMSE at 200, 400, 600, and 800 mg/kg BW/day, respectively, for four weeks. B[a]P exposure significantly (p≤0.05) increased the relative weights of the liver (33.33%) and kidney (39.68%); cytochrome P450 (CYP450) activity, a biotransformation marker, (68.25%); tumor necrosis factor-alpha (TNF-α) levels, a pro-inflammatory mediator, (95.95%); liver enzymes activities (AST, 124.09%, ALT, 124.98%, and ALP, 128.77%); and renal function indicators (serum urea, 60.18%, and creatinine, 68.35%), accompanied by severe histopathologic changes in liver and kidney tissues compared to control (-Ve). Conversely, SMSE interventions significantly (p≤0.05) dosedependently reduced elevated rates of these indices, while improving liver and kidney histoarchitecture. The SMSE's protective effects may result from its bioactive compounds content that regulate B[a]P metabolic enzymes, inhibit CYP450 activity, suppress TNF-α, and scavenge other reactive intermediates. Our results suggest SMSE as a potential natural approach to alleviating environmental pollutants' detrimental effects and deserves further research.

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