Combined effect of exposure to lead and chlordane on the testicular tissues of Swiss mice1The work is part of M.Sc. Thesis of the second author.1

Al‐Omar, Muthanna A.; Abbas, Aamal K; Al-Obaidy, Sabah A

doi:10.1016/s0378-4274(99)00266-0

Cited by 13 publications

(5 citation statements)

References 18 publications

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“…Similarly, reports have been illustrated that the chemicals exposure affects the sperm production in mammals. Al-Omar et al (2000) reported that lead causes decrease in seminiferous tubules diameter in adult rats. Corpas et al (2002) showed that lead acetate causes decrease in the diameter and epithelial thickness of rat seminiferous tubules.…”

Section: Resultsmentioning

confidence: 99%

Autofluorescence in BrdU-Positive Cells and Augmentation of Regeneration Kinetics by Riboflavin

Samuel

Raja

Yesudhason

et al. 2012

Stem Cells and Development

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The earthworm, Eudrilus eugeniae, has a prodigious ability to regenerate lost segments. The skin of the worm has an outermost epidermal layer followed by a thick circular muscle layer and an innermost thin longitudinal cell layer. During the process of regeneration, the circular muscle layer decreased in thickness, and longitudinal cell layer increased. The histological analysis of the regenerated worm shows that the longitudinal cell layer forms the regeneration blastema. BrdU-labeling retention assay confirmed that the circular muscle and longitudinal cell layers have BrdU-positive cells, which migrate from the adjacent segments to the regeneration blastema. In addition, it was noted that the cells of the earthworm, E. eugeniae, have the property of autofluorescence. Autofluorescence was found in the cytoplasm, but not in the nucleus. It has been also found that the major source for autofluorescence is riboflavin. Further, it was also demonstrated that supplementation with riboflavin increases the rate of regeneration, while regeneration was hampered by reduced levels of riboflavin. The importance of riboflavin in regeneration was also confirmed by rescue assay. In addition, it was also identified that BrdU-positive cells are highly fluorescent compared to the surrounding cells.

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Section: Resultsmentioning

confidence: 99%

Autofluorescence in BrdU-Positive Cells and Augmentation of Regeneration Kinetics by Riboflavin

Samuel

Raja

Yesudhason

et al. 2012

Stem Cells and Development

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“…The biologic basis for a chlordane effect on TGCT development is unclear. High doses of chlordane have been reported to have deleterious effects on testicular tissues, with reductions observed in testis weight, seminiferous tubule diameter, and spermatogenesis (Al-Omar et al 2000; Balash et al 1987). Findings from in vitro studies suggest that the influence of chlordane on sex hormone signaling may be complex.…”

Section: Discussionmentioning

confidence: 99%

Prediagnostic Serum Concentrations of Organochlorine Compounds and Risk of Testicular Germ Cell Tumors

Purdue

Engel

Langseth

et al. 2009

Environ Health Perspect

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BackgroundRecent findings suggest that exposure to organochlorine (OC) compounds, chlordanes and p,p′-dichlorodiphenyldichloroethylene (p,p′-DDE) in particular, may increase the risk of developing testicular germ cell tumors (TGCTs).ObjectiveTo further investigate this question, we conducted a nested case–control study of TGCTs within the Norwegian Janus Serum Bank cohort.MethodsThe study was conducted among individuals with serum collected between 1972 and 1978. TGCT cases diagnosed through 1999 (n = 49; 27–62 years of age at diagnosis) were identified through linkage to the Norwegian Cancer Registry. Controls (n =51) were matched to cases on region, blood draw year, and age at blood draw. Measurements of 11 OC insecticide compounds and 34 polychlorinated biphenyl (PCB) congeners were performed using gas chromatography/high-resolution mass spectrometry. Case–control comparisons of lipid-adjusted analyte concentrations were performed using the Wilcoxon signed-rank test. Odds ratios (ORs) and 95% confidence intervals (CIs) for tertiles of analyte concentration were calculated using conditional logistic regression.ResultsTGCT cases had elevated concentrations of p,p′-DDE (tertile 3 vs. tertile 1 OR (ORT3) 2.2; 95% CI, 0.7–6.5; pWilcoxon = 0.07), oxychlordane (ORT3 3.2; 95% CI, 0.6–16.8; pWilcoxon = 0.05), trans-nonachlor (ORT3 2.6; 95% CI, 0.7–8.9; pWilcoxon = 0.07), and total chlordanes (ORT3 2.0; 95% CI, 0.6–7.2; pWilcoxon = 0.048) compared with controls, although no ORs were statistically significant. Seminoma cases had significantly lower concentrations of PCB congeners 44, 49, and 52 and significantly higher concentrations of PCBs 99, 138, 153, 167, 183, and 195.ConclusionsOur study provides additional but qualified evidence supporting an association between exposures to p,p′-DDE and chlordane compounds, and possibly some PCB congeners, and TGCT risk.

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“…Tubular contraction occurs as a result of reduction of fluid secretion and consequently reduction in the overall diameter of the seminiferous tubule. Chlordane is one of the substances that induce a reduction in the diameter of seminiferous tubules [1]. Tubular dilation, with the dilation of tubular lumen may also occur as a result of the increase of the fluid volume in the lumen, or as a consequence of obstruction of fluid flow.…”

Section: Introductionmentioning

confidence: 99%

Segmentation and Morphometry of Histological Sections Using Deformable Models: A New Tool for Evaluating Testicular Histopathology

Guevara

Silva

Oliveira

et al. 2003

Lecture Notes in Computer Science

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Abstract. This paper presents a tool that uses image segmentation and morphometric methods to evaluate testicular toxicity through the analysis of histological sections of mice testis. The tool is based on deformable models (Snakes) and includes several adaptations to solve important difficulties of histological sections imaging, mainly the low contrast edges between the boundary tissue of seminiferous tubules and the interstitial tissue. The method is designed to produce accurate segmentation and to keep track of tubular identities on images under study. The extracted data can be used straightforwardly to compute quantitative parameters characterizing tubular morphology. The method was validated on a realistic data set and the results were compared with those obtained with traditional techniques. The application of this new technique facilitates measurements allowing assessing a higher number of tubules in a fastest and accurate way.

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Combined effect of exposure to lead and chlordane on the testicular tissues of Swiss mice1The work is part of M.Sc. Thesis of the second author.1

Cited by 13 publications

References 18 publications

Autofluorescence in BrdU-Positive Cells and Augmentation of Regeneration Kinetics by Riboflavin

Autofluorescence in BrdU-Positive Cells and Augmentation of Regeneration Kinetics by Riboflavin

Prediagnostic Serum Concentrations of Organochlorine Compounds and Risk of Testicular Germ Cell Tumors

Segmentation and Morphometry of Histological Sections Using Deformable Models: A New Tool for Evaluating Testicular Histopathology

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