Combined effect of Laponite and polymer molecular weight on the cell-interactive properties of synthetic PEO-based hydrogels

Mignon, Arn; Pezzoli, Daniele; Prouvé, Emilie; Lévesque, Lucie; Arslan, Aysu; Pien, Nele; Schaubroeck, David; Hoorick, Jasper Van; Vlierberghe, Sandra Van; Dubruel, Peter

doi:10.1016/j.reactfunctpolym.2018.12.017

Cited by 25 publications

(28 citation statements)

References 73 publications

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“…Furthermore, the introduction of methacrylamides to gelatin (gel-MA) involves a straightforward single step reaction resulting in a plethora of applications. (Figure 1: A) [8,9,25,28,33,[68][69][70][71][72][73][74][75]. Besides this success, other derivatives have also been reported to further tune/improve the material properties.…”

Section: Crosslinking Via Chain-growth Polymerizationmentioning

confidence: 99%

(Photo-)crosslinkable gelatin derivatives for biofabrication applications

et al. 2019

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Over the recent decades gelatin has proven to be very suitable as an extracellular matrix mimic for biofabrication and tissue engineering applications. However, gelatin is prone to dissolution at typical cell culture conditions and is therefore often chemically modified to introduce (photo-)crosslinkable functionalities. These modifications allow to tune the material properties of gelatin, making it suitable for a wide range of biofabrication techniques both as a bioink and as a biomaterial ink (component).The present review provides a non-exhaustive overview of the different reported gelatin modification strategies to yield crosslinkable materials that can be used to form hydrogels suitable for 2 biofabrication applications. The different crosslinking chemistries are discussed and classified according to their crosslinking mechanism including chain-growth and step-growth polymerization.The step-growth polymerization mechanisms are further classified based on the specific chemistry including different (photo-)click chemistries and reversible systems. The benefits and drawbacks of each chemistry are also briefly discussed. Furthermore, focus is placed on different biofabrication strategies applying inkjet, deposition and light-based additive manufacturing techniques, and the applications of the obtained 3D constructs.

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Section: Crosslinking Via Chain-growth Polymerizationmentioning

confidence: 99%

(Photo-)crosslinkable gelatin derivatives for biofabrication applications

et al. 2019

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“…Clay minerals are a group within the phyllosilicates and present in the form of disk-shaped platelets. They are cytocompatible and commonly used as additives to improve mechanical strength and biological effects of soft composites [27,[43][44][45][46][47][48]. In addition, it can improve printability, as recently pioneered by the groups of Gelinsky [49] and Huang [50], which is of particular interest for the present purpose.…”

Section: Graphic Abstract Introductionmentioning

confidence: 99%

Improving printability of a thermoresponsive hydrogel biomaterial ink by nanoclay addition

Hahn

Yang

et al. 2020

J Mater Sci

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As a promising biofabrication technology, extrusion-based bioprinting has gained significant attention in the last decade and major advances have been made in the development of bioinks. However, suitable synthetic and stimuli-responsive bioinks are underrepresented in this context. In this work, we described a hybrid system of nanoclay Laponite XLG and thermoresponsive block copolymer poly(2-methyl-2-oxazoline)-b-poly(2-n-propyl-2-oxazine) (PMeOx-b-PnPrOzi) as a novel biomaterial ink and discussed its critical properties relevant for extrusion-based bioprinting, including viscoelastic properties and printability. The hybrid hydrogel retains the thermogelling properties but is strengthened by the added clay (over 5 kPa of storage modulus and 240 Pa of yield stress). Importantly, the shear-thinning character is further enhanced, which, in combination with very rapid viscosity recovery (~ 1 s) and structure recovery (~ 10 s), is highly beneficial for extrusion-based 3D printing. Accordingly, various 3D patterns could be printed with markedly enhanced resolution and shape fidelity compared to the biomaterial ink without added clay. Graphic abstract

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“…Several studies have focused on measuring and tuning hydrogel stiffness, which characterizes their elasticity, by varying their formulation, [ 14–16 ] by using different copolymers, [ 17,18 ] and interpenetrating networks, [ 19–21 ] or by adding reinforcements. [ 22–24 ] The possibility of controlling these mechanical properties opened the door to several studies on the impact of hydrogel stiffness on cell behavior (adhesion, proliferation, cell differentiation) for regenerative medicine purposes.…”

Section: Introductionmentioning

confidence: 99%

“…[ 22–24 ] The possibility of controlling these mechanical properties opened the door to several studies on the impact of hydrogel stiffness on cell behavior (adhesion, proliferation, cell differentiation) for regenerative medicine purposes. [ 16,18–20,25–28 ] In particular, polyacrylamide hydrogels have been extensively investigated for such studies, [ 29–33 ] as they offer the possibility to simply modulate hydrogel stiffness and adhesive ligand presentation which lead to more complete understanding of cell responses to these stimuli. [ 34 ] For example, Engler and coworkers showed that hMSCs differentiation depends on hydrogel matrix stiffness, with neurogenic differentiation occurring on soft gels mimicking brain mechanical behavior (0.1–1 kPa), myogenic differentiation dominating on gels with mechanical properties close to that of muscle tissues (8–17 kPa), and osteogenic differentiation being favored on stiffer matrices (25–40 kPa).…”

Section: Introductionmentioning

confidence: 99%

Evaluating Poly(Acrylamide‐co‐Acrylic Acid) Hydrogels Stress Relaxation to Direct the Osteogenic Differentiation of Mesenchymal Stem Cells

Prouvé

Drouin

Chevallier

et al. 2021

Macromolecular Bioscience

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The aim of this study is to investigate polyacrylamide‐based hydrogels stress relaxation and the subsequent impact on the osteogenic differentiation of human mesenchymal stem cells (hMSCs). Different hydrogels are synthesized by varying the amount of cross‐linker and the ratio between the monomers (acrylamide and acrylic acid), and characterized by compression tests. It has been found that hydrogels containing 18% of acrylic acid exhibit an average relaxation of 70%, while pure polyacrylamide gels show an average relaxation of 15%. Subsequently, hMSCs are cultured on two different hydrogels functionalized with a mimetic peptide of the bone morphogenetic protein‐2 to enable cell adhesion and favor their osteogenic differentiation. Phalloidin staining shows that for a constant stiffness of 55 kPa, a hydrogel with a low relaxation (15%) leads to star‐shaped cells, which is typical of osteocytes, while a hydrogel with a high relaxation (70%) presents cells with a polygonal shape characteristic of osteoblasts. Immunofluorescence labeling of E11, strongly expressed in early osteocytes, also shows a dramatically higher expression for cells cultured on the hydrogel with low relaxation (15%). These results clearly demonstrate that, by fine‐tuning hydrogels stress relaxation, hMSCs differentiation can be directed toward osteoblasts, and even osteocytes, which is particularly rare in vitro.

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Combined effect of Laponite and polymer molecular weight on the cell-interactive properties of synthetic PEO-based hydrogels

Cited by 25 publications

References 73 publications

(Photo-)crosslinkable gelatin derivatives for biofabrication applications

(Photo-)crosslinkable gelatin derivatives for biofabrication applications

Improving printability of a thermoresponsive hydrogel biomaterial ink by nanoclay addition

Evaluating Poly(Acrylamide‐co‐Acrylic Acid) Hydrogels Stress Relaxation to Direct the Osteogenic Differentiation of Mesenchymal Stem Cells

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