Combined effect of low-power laser irradiation and anthraquinone anticancer drug aclarubicin on survival of immortalized cells: Comparison with mitoxantrone

Abstract

The photodynamic response of the anthraquinone anticancer drug aclarubicin (ACL) was investigated in vitro and compared with that of mitoxantrone (MTX). Cultured immortalized rodent B14 and NIH 3T3 cells were used in the experiments as a model for cells with neoplastic phenotype. Long-term cytotoxicity and inhibition of cell proliferation assayed by the clonal growth and MTT-tetrazolium methods were estimated to compare the efficacy of aclarubicin and mitoxantrone in photosensitizing cells and their death after non-thermal exposure to monochromatic laser light. Green He-Ne (543.5 nm) or red semiconductor (670 nm) low-power laser (LPL) irradiations were applied. Different dose-responses of both cell lines to aclarubicin and mitoxantrone were found so that the cytotoxicity of MTX was considerably greater than the cytotoxicity of ACL. Phototherapy response (P < 0.0001) was observed only for B14 cells after sensitisation with aclarubicin. Under the same conditions no significant effect of red light irradiation (semiconductor 670 nm laser) on survival of both cell lines treated with mitoxantrone was found.

Uredinorubellins and Caeruleoramularin, Photodynamically Active Anthraquinone Derivatives Produced by Two Species of the Genus Ramularia

“…Because of the well-known photodynamic activity of the rubellins ,, together with the data concerning the biological activity of anthraquinone derivatives, – we investigated the performance of these new compounds in several test systems, to ascertain whether their activity is caused by photodynamic effects.…”

Avaliação da citotoxicidade e fototoxicidade da riboflavina

“…A terapia fotodinâmica é caracterizada pelo acúmulo de uma substância fotossensibilizadora em um tecido especialmente iluminado e, subseqüentemente, destruído (Edwards et al, 1994) RF + H 2 O 2 1 RF* fotossensibilizadores induzem reação fotoquímica do tipo II, que levam à produção de agentes citotóxicos, especialmente oxigênio singlete. A reação fotoquímica do tipo I também pode ser induzida pelos fotossensibilizadores e as substâncias tóxicas geradas podem causar peroxidação lipídica, danos ao ácido desoxirribonucleico (DNA), à membrana plasmática, ao citoesqueleto e outras organelas subcelulares (Koceva-Chyla et al, 2006). A irradiação com luz UV é considerada importante estratégia terapêutica em dermatologia e tem melhorado dramaticamente o tratamento de doenças de pele.…”