Combined effect of permeant and non-permeant cryoprotectants on the quality of frozen/thawed chicken sperm

Mosca, Fabio; Madeddu, Manuela; Sayed, Ahmad Abdel; Zaniboni, L.; Iaffaldano, Nicolaia; Cerolini, S.

doi:10.1016/j.cryobiol.2016.10.001

Cited by 48 publications

(47 citation statements)

References 39 publications

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“…However, some sperm motility parameters were shown to be positively affected by non penetrating cryoprotectants, trehalose alone or in combination with sucrose. Our results are similar to the report by Mosca et al [17] where sucrose combined with DMA had no influence on post thaw sperm parameters. Species difference in effect of sucrose and trehalose was observed where these osmoprotectants improved post thaw sperm motility in turkey but not in crane [16].…”

Section: Discussionsupporting

confidence: 93%

“…[15] reported no effect of trehalose on post thaw sperm parameters when added along with penetrating cryoprotectant in rooster and partridge semen. Combining trehalose and sucrose with DMA and straw packing did not affect post thaw chicken sperm viability and progressive motility [17]. However, some sperm motility parameters were shown to be positively affected by non penetrating cryoprotectants, trehalose alone or in combination with sucrose.…”

Section: Discussionmentioning

confidence: 83%

“…The non permeable cryoprotectants trehalose and sucrose were studied in chicken semen cryopreservation [14,15,17]. Preliminary studies in our laboratory to vitrify chicken semen using sucrose (up to 300 mM) alone as cryoprotectant did not produce any post thaw viable sperm.…”

Section: Discussionmentioning

confidence: 99%

“…A combination of penetrating and non penetrating cryoprotectants was tested during semen cryopreservation in rooster and other species [14][15][16][17]. The penetrating cryoprotectants protect cells from cryoinjury by increasing membrane fluidity and reducing the intracellular ice crystal formation [18].…”

Section: Introductionmentioning

confidence: 99%

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Pellet Method of Semen Cryopreservation: Effect of Cryoprotectants, Semen Diluents and Chicken Lines

Murugesan

Mahapatra

2019

Braz. arch. biol. technol.

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The present study evaluated the effect of cryoprotectants, semen diluents and chicken lines during pellet method of semen cryopreservation. Three different experiments were conducted; Experiment 1 -semen was cryopreserved using dimethylformamide (DMF) at 6% and 9% concentrations in two semen diluents (Lake and Ravie diluent and TES/NaCl diluent), Experiment 2 -semen was cryopreserved using dimethylacetamide (DMA) at 6% and 9% with or without sucrose (100mM), Experiment 3-semen from two chicken lines (PD1 and PD6) was cryopreserved using DMA (6% and 9%). Semen was evaluated pre and post cryopreservation for progressive motility, live and abnormal sperm. Semen pellets were stored in cryovials for at least seven days before examination and insemination. Thawed semen was inseminated intravaginaly to study fertility. All the parameters studied were significantly lower (p<0.05) in cryopreserved semen. DMF in Lake and Ravie diluent gave very low fertility and TES/NaCl diluent no fertile eggs. DMA as cryoprotectant gave fertility up to 9.22 %. Addition of sucrose along with DMA produced fertility similar to other cryopreservation treatment groups. No difference in in vitro semen parameters between chicken lines was observed. There is difference in cryopreservation outcome due to semen diluent and type of cryoprotectant. HIGHLIGHTS Cryoprotectants, diluents and chicken lines effect in semen vitrification evaluated  Dimethylformamide gave low fertility in Lake and Ravie diluent and no fertility in TES diluent  Dimethylacetmide gave fertility up to 9.22 % and along with sucrose produced similar fertility  Post thaw in vitro semen parameters similar between chicken lines Shanmugam, M; Mahapatra ,R.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pellet Method of Semen Cryopreservation: Effect of Cryoprotectants, Semen Diluents and Chicken Lines

Murugesan

Mahapatra

2019

Braz. arch. biol. technol.

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“…Taken together, our data strongly recommend the use of trehalose as an additive to the conventional freezing medium to perpetuate the functions of DCs through cryopreservation. The future utility of this study will be trying a combination of trehalose and other CPAs that have been already reported for other cell systems for freezing of DCs. It is possible that these combinations may further improve the efficacy of cryopreservation of DCs.…”

Section: Discussionmentioning

confidence: 99%

Freezing of dendritic cells with trehalose as an additive in the conventional freezing medium results in improved recovery after cryopreservation

et al. 2018

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BACKGROUND: Dendritic cell (DC) vaccination involves administration of multiple doses. Cryopreservation of tumor antigen-pulsed DCs can provide a ready to use vaccine source and eliminate the need of frequent withdrawal of the patient's blood for vaccine preparation. The aim of this study was to assess the effect of addition of trehalose in the freezing medium on the recovery of DCs after cryopreservation.STUDY DESIGN AND METHODS: DCs were generated from mononuclear cells from apheresis samples of healthy donors. For long-term storage of 6 months, cells were frozen with a rate-controlled programmable freezer and stored in liquid nitrogen. For short-term storage of 1 month, cells were frozen and stored at −80 C. DCs frozen with Iscove's Modified Dulbecco's Medium + 10% dimethyl sulfoxide + 20% fetal bovine serum served as the control group, while the test group was additionally supplemented with 50 μg/mL of trehalose. After revival of control and test DCs, they were assessed for viability, morphology, phenotype, and functions. RESULTS:The addition of trehalose to the conventional freezing medium helped to preserve the viability and functionality of DCs better than dimethyl sulfoxide alone in both long-and short-term cryopreservation. Trehalose also protected the mitochondrial membrane potential and cytoskeleton integrity of DCs, which are necessary for their functionality. Mediators of the intrinsic apoptotic pathway like Caspase-9 and Bim-1 were found to be low in the test. CONCLUSION: Supplementation of conventional freezing medium with trehalose results in better quality of DCs revived after cryopreservation. This finding could help improve DC vaccine preparation for cancer immunotherapy. ABBREVIATIONS: CFSE = carboxyfluorescein succinimidyl ester; CPAs = cryoprotective agents; CTL = cytotoxic T-lympocyte; DC = dendritic cell; DMSO = dimethyl sulfoxide; FBS = fetal bovine serum; HLA = human leukocyte antigen; IMDM = Iscove's Modified Dulbecco's Medium; NCCS = National Centre for Cell Science; PBS = phosphate-buffered saline; PCR = polymerase chain reaction; TMRE = tetramethylrhodamine, ethyl ester. From the

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Reproductive technologies in avian species

Barna¹,

Végi²,

Liptói³

et al. 2020

Reproductive Technologies in Animals

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Combined effect of permeant and non-permeant cryoprotectants on the quality of frozen/thawed chicken sperm

Cited by 48 publications

References 39 publications

Pellet Method of Semen Cryopreservation: Effect of Cryoprotectants, Semen Diluents and Chicken Lines

Pellet Method of Semen Cryopreservation: Effect of Cryoprotectants, Semen Diluents and Chicken Lines

Freezing of dendritic cells with trehalose as an additive in the conventional freezing medium results in improved recovery after cryopreservation

Reproductive technologies in avian species

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