Combined effect of retinoic acid and calcium on the <i>in vitro</i> differentiation of human adipose-derived stem cells to adipocytes

Goudarzi, Farjam; Sarveazad, Arash; Mahmoudi, Maryam; Mohammadalipour, Adel; Chahardoli, Reza; Malekshah, Obeid M.; Karimi, Shiva; Saboor-Yaraghi, Ali Akbar

doi:10.1080/13813455.2017.1367009

Cited by 9 publications

(6 citation statements)

References 43 publications

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“…In summary, the cells from passage four were trypsinized and transferred to a 15 ml tube and xed with 4% paraformaldehyde. Finally, surface CD markers were characterized using a ow cytometry device (Partec, Germany) [18].…”

Section: Hmscs Immunophenotyping and Multipotency Assaysmentioning

confidence: 99%

“…For chondrogenesis, the chondrogenic medium was used which contained the same complete medium and dexamethasone (100 nM), L-proline (40 µg/ml; Merck Millipore Corporation, Istanbul, Turkey), L-ascorbic acid 2-phosphate (50 µg/ml; Sigma-Aldrich, Germany), and TGF-β3 (10 ng/ml; BioLegend Ò , Istanbul-Turkey). For adipogenesis, the adipogenic medium contained dexamethasone (1 µM), indomethacin (100 µM), insulin (10 µg/mL), and 3-isobutyl-1-methylxanthine (IBMX; 0.5 mM) (All from Sigma-Aldrich Co. Ltd., Dorset, UK) [17,18].…”

Section: Cell Culturementioning

confidence: 99%

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WITHDRAWN: Apigenin neutralizes the inhibitory effect of inflammation on the osteogenic differentiation of human mesenchymal stem cells

Asadi

Goudarzi

Ghanadian

et al. 2021

Preprint

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Background: The stimulating effects of apigenin on mesenchymal stem cells (MSCs) osteogenesis, as well as the anti-inflammatory effect of this flavonoid, have been identified. In this study, osteogenic differentiation was investigated under inflammatory conditions and treatment with apigenin. Methods and Results: Along with osteogenic differentiation of MSCs, they became inflamed with LPS/PA, and treated simultaneously with apigenin. The degree of differentiation was assessed by alizarin red staining and alkaline phosphatase (ALP) activity. Also, gene expression of NLRP3 and RUNX2 was performed along with protein expression of IL-1β. Significant increase in NLRP3 and IL-1β were observed in MSCs when exposed to LPS/PA (p<0.01). Also, the osteogenesis was significantly decreased (p<0.01). Apigenin treatment induced significantly higher gene expression of RUNX2, the activity of ALP, and cell staining (p<0.01) which were also associated with reduced inflammation in these cells. Conclusions: The effectiveness of apigenin on osteogenesis under inflammatory conditions was cautiously observed.

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Section: Hmscs Immunophenotyping and Multipotency Assaysmentioning

confidence: 99%

Section: Cell Culturementioning

confidence: 99%

WITHDRAWN: Apigenin neutralizes the inhibitory effect of inflammation on the osteogenic differentiation of human mesenchymal stem cells

Asadi

Goudarzi

Ghanadian

et al. 2021

Preprint

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“…Some animal studies show that xenograft transplantation of mesenchymal stem cells can reduce the immunomodulation [48] and efficacy of these cells [49]. However, the use of hADSCs has been usual in preclinical studies [50–52] and its efficacy is proven. The findings of the present study also show that xenograft transplantation of hADSCs in the anal sphincterotomy model in rabbit improves sphincter function and tissue repair.…”

Section: Discussionmentioning

confidence: 99%

Combination of laser and human adipose-derived stem cells in repair of rabbit anal sphincter injury: a new therapeutic approach

et al. 2019

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BackgroundAnal sphincter injury leads to fecal incontinence. Based on the regenerative capability of laser and human adipose-derived stem cells (hADSCs), this study was designed to assess the effects of co-application of these therapies on anal sphincter recovery after injury.DesignMale rabbits were assigned to equal groups (n = 7) including control, sphincterotomy, sphincterotomy treated with laser (660 nm, 90 s, immediately after sphincterotomy, daily, 14 days), hADSCs (2 × 106 hADSCs injected into injured area of the sphincter immediately after sphincterotomy), and laser + hADSCs. Ninety days after sphincterotomy, manometry and electromyography were performed, sphincter collagen content was evaluated, and Ki67, myosin heavy chain (MHC), skeletal muscle alpha-actin (ACTA1), vascular endothelial growth factor A (VEGFA), and vimentin mRNA gene expression were assessed.ResultsThe laser + hADSCs group had a higher resting pressure compared with the sphincterotomy (p < 0.0001), laser (p < 0.0001), and hADSCs (p = 0.04) groups. Maximum squeeze pressure was improved in all treated animals compared with the sphincterotomized animals (p < 0.0001), without a significant difference between treatments (p > 0.05). In the laser + hADSCs group, motor unit numbers were higher than those in the laser group (p < 0.0001) but did not differ from the hADSCs group (p = 0.075). Sphincterotomy increased collagen content, but the muscle content (p = 0.36) and collagen content (p = 0.37) were not significantly different between the laser + hADSCs and control groups. Laser + hADSCs increased ACTA1 (p = 0.001) and MHC (p < 0.0001) gene expression compared with laser or hADSCs alone and was associated with increased VEGFA (p = 0.009) and Ki67 mRNA expression (p = 0.01) and decreased vimentin mRNA expression (p < 0.0001) compared with laser.ConclusionThe combination of laser and hADSCs appears more effective than either treatment alone for promoting myogenesis, angiogenesis, and functional recovery after anal sphincterotomy.

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“…The sterile samples were kept in a tube including DMEM/F12, FBS 10%, and penicillin/streptomycin (P/S) 5%. Like our previous works (18,19), we used a protocol described by Dubois et al for the isolation of stem cells (20). Before extracting, the fat samples were heated to 37°C in a water bath.…”

Section: Isolation and Culture Of Hadscsmentioning

confidence: 99%

Histological and Behavioral Alterations Following hADSCs Intravenous Administration in Alzheimer’s Rat Model

Kazemiha

Sarveazad

Moradi

et al. 2020

Thrita

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Background: Alzheimer's disease (AD) is a progressive cognitive disorder that is generally age-related. Although there has been great research focusing on this disease, there is still a lack of reliable therapeutic methods. Amyloid-β (Aβ) peptide has a critical function in neuropathology of AD. Stem cell therapy provides treatment by improving the neuronal system in neurodegenerative disorders. Human adipose-derived stem cells (hADSCs) are the most appropriate sources of stem cells due to their safety, high proliferative potential, and easy isolation. Objectives: The present study was designed to evaluate the histological and behavioral alterations after intravenous administration of hADSCs in the AD rat model. Methods: In this study, 32 male rats were used in four groups, as follows: control, sham, AD rat model, and hADSCs-treated group. We used Morris Water Maze (MWM) for evaluating behavioral changes and Nissl staining for determining the histological studies. Results: In this study, the AD model was confirmed by behavioral and histological analysis. Behavioral results showed that the spatial memory improved after hADSCs injection in the AD rat model while the time spent in the target quadrant was significantly higher in the hADSCs-treated group than in the AD rat model group. On the other hand, the number of dead cells significantly decreased in the hADSCs-treated group as analyzed by Nissl staining. Conclusions: Our findings revealed that hADSCs could transfer into the brain and improve memory and neuronal damage in the AD rat model.

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Combined effect of retinoic acid and calcium on the in vitro differentiation of human adipose-derived stem cells to adipocytes

Abstract: These results suggest that ATRA and calcium together have significant inhibitory effect on adipogenesis that can be helpful for finding new mechanisms to prevent or control the adipogenesis.

Cited by 9 publications

References 43 publications

WITHDRAWN: Apigenin neutralizes the inhibitory effect of inflammation on the osteogenic differentiation of human mesenchymal stem cells

WITHDRAWN: Apigenin neutralizes the inhibitory effect of inflammation on the osteogenic differentiation of human mesenchymal stem cells

Combination of laser and human adipose-derived stem cells in repair of rabbit anal sphincter injury: a new therapeutic approach

Histological and Behavioral Alterations Following hADSCs Intravenous Administration in Alzheimer’s Rat Model

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