Combined effect of salinomycin and feeding on whole body glucose kinetics in sheep fed a high-concentrate diet

Abstract

-The aim of this study was to investigate the effects of salinomycin (SL) and feeding on whole body glucose kinetics in sheep fed a high-concentrate diet (25% orchardgrass hay and 75% commercial concentrate). Four adult sheep were fed the diet with or without 20 mg·kg −1 diet of SL once daily for each 3 wk. The rates of glucose entry and utilization were determined before and during 3 h after feeding using a [ 13 C 6 ]glucose dilution approach. Ruminal characteristics and concentrations of blood volatile fatty acids (VFA) and plasma glucose and insulin were also measured. Metabolizable energy intake was unaffected (P = 0.22) with SL. Salinomycin decreased (P = 0.06) the ratio of acetate to propionate in rumen fluid. Salinomycin increased (P = 0.01) both rates of entry and utilization of glucose, but did not affect (P > 0.10) concentrations of blood VFA or plasma glucose or insulin. Feeding caused gradual increases in concentrations of blood acetate (P < 0.01) and propionate (P = 0.01), a transient increase in plasma insulin concentration (P = 0.05), a transient decrease in plasma glucose concentration (P < 0.01), and persistent increases in both rates of glucose entry (P < 0.01) and utilization (P < 0.01). No SL × feeding interaction was observed (P > 0.10) on any measurements. We conclude that SL and feeding would have an additive effect on both rates of glucose entry and utilization without modifications with SL to feeding responses of peripheral concentrations of blood VFA, plasma glucose and insulin.feeding / glucose metabolism / insulin / ionophore / propionate / sheep

Effect of Dietary Protein Intake on Turnover and Oxidation Rates of Plasma Glucose in Adult Sheep

“…Plasma glucose turnover rate was almost comparable t o those obtained previously in sheep using similar experimental techniques (Fujita et al, 2006). Barry and Manley (1985) reported t h a t casein infusion into the abomasum increased glucose irreversible loss rate in late-pregnant ewes, even metabolizable energy intake was constant.…”

“…Glucose was isolated with ion exchange resin (Dowex 50W-X8, Dowex 1x8, Bio-Rad Lab., USA) and was derivatized to glucose aldonitrile pentaacetate according to the procedure of Tserng and Kalhan (1983) with slight modifications (Fujita et al, 2006). The isotopic abundances of plasma [U-13C]-glucose derivatives and 13C0, were determined by the gas chromatographycombustion-isotope ratio mass spectrometry (DELTAplus, Thermo Electron Corp., USA).…”

“…Turnover and oxidation rates of plasma glucose were calculated using the equations described by Fujita et al (2006). Total heat production was calculated from the Brouwer's equation (Brouwer, 1965) using 0, consumption and CO, production a s described by Young et al (1975).…”

Effects of Chinese herbal medicine on plasma glucose, protein and energy metabolism in sheep

“…[12]. Enrichments of plasma [U- 13 C]glucose were measured by gas chromatography mass spectrometry (QP-2010, Shimadzu, Japan).…”

Effects of Chinese herbal medicine and cold exposure on plasma glucose, leucine and energy metabolism in sheep

“…Plasma glucose was derivatized to glucose aldonitrile pentaacetate according to the procedure of Tserng and Kalhan (1983) with slight modifications by Fujita, Majima, Itoh, and Sano (2006b). Enrichment of plasma [U-13 C]glucose was measured by gas chromatography-mass spectrometry (QP-2010, Shimadzu, Japan).…”