Combined effect of titanium particles and TNF-? on the production of IL-6 by osteoblast-like cells

Abstract

To clarify the role of tumor necrosis factor (TNF)-alpha on osteoblast functions in the presence of metal particles, two human osteoblast-like cell lines (MG-63 and SaOS-2) were cultured with TNF-alpha in the presence or absence of titanium particles in vitro. A combination of TNF-alpha and titanium particles showed additive effects on inhibition of cell proliferation and alkaline phosphatase production. On the other hand, production of interleukin-6, which is well known to induce osteoclastogenesis and to directly stimulate bone resorption, was additively stimulated by the combination of TNF-alpha and titanium particles. These results suggest that the association of TNF-alpha and titanium particles may play an important role in the pathogenesis of periprosthetic osteolysis through two different pathways: a reduced periprosthetic bone formation due to inhibition of osteoblast proliferation and alkaline phosphatase production, and osteoblast-mediated activation of osteoclastic bone resorption as suggested by the enhancement of interleukin-6 production.

The metal nanoparticle-induced inflammatory response is regulated by SIRT1 through NF-κB deacetylation in aseptic loosening

“…Based on previous studies concerning inflammatory cytokines and bone metabolism and the observation that both TiPs and CoPs were able to stimulate the production of TNF-α and IL-1β in this study, it was reasonable to speculate that TNF-α and IL-1β, produced by macrophages after the stimulation of metal nanoparticles, mediated the balance between bone resorption and formation in the pathological process of aseptic loosening ( Figure S2). 4,[34][35][36][37][38][39] SIRT1 is an important HDAC due to its proposed role in the association between calorie restriction and lifespan extension. SIRT1 is also a major regulator of bone mass.…”

Drp1-dependent mitochondrial fission mediates osteogenic dysfunction in inflammation through elevated production of reactive oxygen species

“…In order to choose an appropriate working concentration of TNF-α, a range of concentrations (0, 5, 10, 100 ng/ml) was used in preliminary experiments based on previous reports [7, 8, 10, 13]. Based on the preliminary results, 10 ng/ml TNF-α was used as a stimulator of osteoblast inflammatory model (data listed below).…”

“…Tumor necrosis factor-α (TNF-α), is a well-known bone-acting cytokine that is involved in the progression and severity of inflammation-induced bone destruction [7–10]. Stimulation of RANKL/OPG ratio and/or activation of NF-κB in osteoblasts are recognized as the signals responsible for TNF-α-triggered osteolysis [11–13]. However, the TNF-α regulatory mechanisms associated with impaired osteogenic function remain largely unknown.…”

The influence of wear particles in the expression of osteoclastogenesis factors by osteoblasts

“…We showed that particles had a cytotoxic effect [10], modulated the fibronectin gene expression [11], and decreased the adhesion strength [12] of osteoblasts. Moreover, combination of particles and cytokines had a synergic effect on the production of inflammatory factors [13]. Using microarray techniques, it has been shown that particles had a profound impact on genes coding for inflammatory cytokines and genes controlling the nuclear architecture [14].…”