Combined effects of different nitrogen sources and levels and light intensities on growth and fatty acid and lipid production of oleaginous eustigmatophycean microalga Eustigmatos cf. polyphem

Gao, Baoyan; Xia, Song; Lei, Xueqing; Zhang, Chengwu

doi:10.1007/s10811-017-1180-9

Cited by 17 publications

(8 citation statements)

References 51 publications

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“…In another report, there was increased biomass production of Eustigmatos cf. polyphem at increased light intensity from low light intensity (100 μmol photons m −2 s −1 ) to high light intensity (300 μmol photons m −2 s −1 ) with all the nitrogen sources tested (Gao et al 2017 ). However, Pozzobon et al ( 2020 ) recently reported that during cultivation of Desmodesmus sp.…”

Section: Resultsmentioning

confidence: 99%

Comparison of growth, protein and carotenoid contents of some freshwater microalgae and the effects of urea and cultivation in a photobioreactor with reflective broth circulation guide on Desmodesmus subspicatus LC172266

Eze

Ogbonna

Aoyagi

et al. 2021

Braz. J. Chem. Eng.

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This study assessed the potential of enhancing carotenoids and protein accumulation by Desmodesmus subspicatus LC172266 by urea supplementation and cultivation in a novel flat plate air-lift photobioreactor with broth circulation guides. Addition of 0.2 g L −1 urea to BG11 medium significantly increased the cell growth in both flask and photobioreactor cultures. Carotenoid accumulation by D. subspicatus was about 5.7 times (15.5 ± 0.1 mgg cell −1 ) higher in the photobioreactor with reflective broth circulation guide than in the flask culture (2.3 ± 0.1 mgg −1 cell) and about 46% higher than the carotenoid content obtained in the photobioreactor without broth circulation guide (control).The cell growth rate was about 2 times higher in the photobioreactor with reflective broth circulation guide (0.41 ± 0.005 day −1 ) than in flask culture (0.20 day −1 ), and in the control photobioreactor (0.28 day −1 ).Both the protein contents and biomass concentrations were significantly enhanced by cultivation in the novel photobioreactor. This research has demonstrated that the flat plate air-lift photobioreactor installed with reflective broth circulation guide substantially enhanced carotenoid production by D. subspicatus LC172266 .

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Section: Resultsmentioning

confidence: 99%

Comparison of growth, protein and carotenoid contents of some freshwater microalgae and the effects of urea and cultivation in a photobioreactor with reflective broth circulation guide on Desmodesmus subspicatus LC172266

Eze

Ogbonna

Aoyagi

et al. 2021

Braz. J. Chem. Eng.

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“…Our previous work found that E. cf. polyphem can accumulate large quantities of lipids and β-carotene under stress conditions [ 18 , 19 ], and because of its high lipid productivity production, its photosynthetic apparatus is worthy of closer examination. The eustigmatophycean microalgae lack chlorophylls b and c , and they mainly contain chlorophyll a , violaxanthin, vaucheriaxanthin ester, and β-carotene as photosynthetic pigments [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Optimum Production Conditions, Purification, Identification, and Antioxidant Activity of Violaxanthin from Microalga Eustigmatos cf. polyphem (Eustigmatophyceae)

et al. 2018

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Violaxanthin is a major xanthophyll pigment in the microalga Eustigmatos cf. polyphem, but the amount produced after propagation can vary depending upon culture conditions. In this study, the effects of cultivation time, nitrogen concentration, light intensity, and culture mode on violaxanthin production were investigated. The results showed that this microalga vigorously grew and maintained a high level of violaxanthin in the fed-batch culture, and the highest violaxanthin productivity of 1.10 ± 0.03 mg L−1 d−1 was obtained under low light illumination with 18 mM of initial nitrogen supply for ten days. Additionally, violaxanthin was purified from E. cf. polyphem by silica gel chromatography and preparative high-performance liquid chromatography (PHPLC), and identified with high-resolution mass spectrometry (HRMS). The antioxidant activity of the purified violaxanthin was evaluated by three tests in vitro: reducing power assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2-azobis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical assays. The strongest inhibition of purified violaxanthin occurred during the scavenging of ABTS+ radicals, with EC50 of 15.25 μg mL−1. In conclusion, this is the first report to investigate the effects of different culture conditions on violaxanthin accumulation in E. cf. polyphem and provide a novel source for the production of violaxanthin that can be used for food and pharmaceutical applications.

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“…Lipids are the most acknowledged reserve material found in eustigmatophytes and are of biotechnological importance, especially for biofuel and food purposes (Gao et al. ). The accumulation of lipid droplets in the cytoplasm often has been reported (Schnepf et al.…”

Section: Discussionmentioning

confidence: 99%

“…The need to clarify the diversity and phylogeny of the Eustigmatophyceae and to provide proper identifications of strains held in culture collections is now more urgent than ever before, given the rapid growth of interest in eustigmatophytes other than Nannochloropsis and Microchloropsis that have been stimulated by the fact that all studied Eustigmatophyceae produce valuable compounds such as lipids (Pal et al. , Gao et al , ), carotenoids (Lubián et al. , Li et al.…”

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confidence: 99%

Toward Modern Classification of Eustigmatophytes, Including the Description of Neomonodaceae Fam. Nov. and Three New Genera¹

Amaral

Fawley

Němcová

et al. 2020

Journal of Phycology

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The class Eustigmatophyceae includes mostly coccoid, freshwater algae, although some genera are common in terrestrial habitats and two are primarily marine. The formal classification of the class, developed decades ago, does not fit the diversity and phylogeny of the group as presently known and is in urgent need of revision. This study concerns a clade informally known as the Pseudellipsoidion group of the order Eustigmatales, which was initially known to comprise seven strains with oval to ellipsoidal cells, some bearing a stipe. We examined those strains as well as 10 new ones and obtained 18S rDNA and rbcL gene sequences. The results from phylogenetic analyses of the sequence data were integrated with morphological data of vegetative and motile cells. Monophyly of the Pseudellipsoidion group is supported in both 18S rDNA and rbcL trees. The group is formalized as the new family Neomonodaceae comprising, in addition to Pseudellipsoidion, three newly erected genera. By establishing Neomonodus gen. nov. (with type species Neomonodus ovalis comb. nov.), we finally resolve the intricate taxonomic history of a species originally described as Monodus ovalis and later moved to the genera Characiopsis and Pseudocharaciopsis. Characiopsiella gen. nov. (with the type species Characiopsiella minima comb. nov.) and Munda gen. nov. (with the type species Munda aquilonaris) are established to accommodate additional representatives of the polyphyletic genus Characiopsis. A morphological feature common to all examined Neomonodaceae is the absence of a pyrenoid in the chloroplasts, which discriminates them from other morphologically similar yet unrelated eustigmatophytes (including other Characiopsis‐like species).

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Combined effects of different nitrogen sources and levels and light intensities on growth and fatty acid and lipid production of oleaginous eustigmatophycean microalga Eustigmatos cf. polyphem

Cited by 17 publications

References 51 publications

Comparison of growth, protein and carotenoid contents of some freshwater microalgae and the effects of urea and cultivation in a photobioreactor with reflective broth circulation guide on Desmodesmus subspicatus LC172266

Comparison of growth, protein and carotenoid contents of some freshwater microalgae and the effects of urea and cultivation in a photobioreactor with reflective broth circulation guide on Desmodesmus subspicatus LC172266

Optimum Production Conditions, Purification, Identification, and Antioxidant Activity of Violaxanthin from Microalga Eustigmatos cf. polyphem (Eustigmatophyceae)

Toward Modern Classification of Eustigmatophytes, Including the Description of Neomonodaceae Fam. Nov. and Three New Genera¹

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Combined effects of different nitrogen sources and levels and light intensities on growth and fatty acid and lipid production of oleaginous eustigmatophycean microalga Eustigmatos cf. polyphem

Cited by 17 publications

References 51 publications

Comparison of growth, protein and carotenoid contents of some freshwater microalgae and the effects of urea and cultivation in a photobioreactor with reflective broth circulation guide on Desmodesmus subspicatus LC172266

Comparison of growth, protein and carotenoid contents of some freshwater microalgae and the effects of urea and cultivation in a photobioreactor with reflective broth circulation guide on Desmodesmus subspicatus LC172266

Optimum Production Conditions, Purification, Identification, and Antioxidant Activity of Violaxanthin from Microalga Eustigmatos cf. polyphem (Eustigmatophyceae)

Toward Modern Classification of Eustigmatophytes, Including the Description of Neomonodaceae Fam. Nov. and Three New Genera1

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Toward Modern Classification of Eustigmatophytes, Including the Description of Neomonodaceae Fam. Nov. and Three New Genera¹