Combined Effects of Electric Stimulation and Microgrooves in Cardiac Tissue‐on‐a‐Chip for Drug Screening

Ren, Li; Zhou, Xingwu; Nasiri, Rohollah; Fang, Jun; Jiang, Xing; Wang, Canran; Qu, Moyuan; Ling, Haonan; Chen, Yihang; Xue, Yumeng; Hartel, Martin C.; Tebon, Peyton; Zhang, Shiming; Kim, Hanjun; Yuan, Xiaocong; Shamloo, Amir; Dokmeci, Mehmet R.; Song, Li; Khademhosseini, Ali; Ahadian, Samad; Sun, Wujin

doi:10.1002/smtd.202000438

Cited by 24 publications

(20 citation statements)

References 49 publications

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“…We chose clinically approved DOX and DEX as model drugs to examine dose‐dependent cardiotoxicity. [ 46 ] DOX is a commonly used chemotherapy drug with myocardial toxicity. [ 47 ] One common side effect of DOX is increased heart rate, and long‐term use can induce severe heart damage.…”

Section: Resultsmentioning

confidence: 99%

Multiscale Anisotropic Scaffold Integrating 3D Printing and Electrospinning Techniques as a Heart‐on‐a‐Chip Platform for Evaluating Drug‐Induced Cardiotoxicity

Liu,

Wang,

Chen

et al. 2023

Adv Healthcare Materials

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Cardiac safety assessments are significant in drug discovery, as drug‐induced cardiotoxicity (DIC) is the primary cause of drug attrition. Despite heart‐on‐a‐chip (HoC) technology becoming an increasingly popular tool for evaluating DIC, its development remains a challenge owing to the anisotropic cardiac structure of the native myocardium. Herein, an anisotropic multiscale cardiac scaffold is presented via a hybrid biofabrication method by combining 3D printing with electrospinning technology, where the 3D‐printed micrometer‐scale scaffold frames enable mimicking the interwoven myocardium anatomical structure and the branched‐aligned electrospun nanofibers network is able to directionally guide cellular arrangements. The in vitro 3D bioengineered cardiac tissues are then fabricated by encapsulating three‐layer multiscale scaffolds within a photocurable methacrylated gelatin hydrogel shell. It is demonstrated that such an anisotropic multiscale structure could contribute to enhancing cardiomyocyte maturation and synchronous beating behavior. More attractively, with the integration of 3D bioengineered cardiac tissues and a self‐designed microfluidic perfusion system, a 3D anisotropic HoC platform is established for evaluating DIC and cardioprotective efficacy. Collectively, these results indicate that the HoC model developed by integrating the 3D bioengineered cardiac tissues could effectively recapitulate the clinical manifestations, thereby highlighting their efficacy as a valuable preclinical platform for testing drug efficacy and cardiotoxicity.

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Section: Resultsmentioning

confidence: 99%

Multiscale Anisotropic Scaffold Integrating 3D Printing and Electrospinning Techniques as a Heart‐on‐a‐Chip Platform for Evaluating Drug‐Induced Cardiotoxicity

Liu,

Wang,

Chen

et al. 2023

Adv Healthcare Materials

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“…Ren et al . fabricated a heart-on-a-chip for high-throughput drug screening[ 92 ]. They chose clinically approved doxorubicin and cyclophosphamide as model drugs to examine dose-dependent cardiotoxicity, and ivabradine and carbachol as candidates for ameliorating cardiotoxicity.…”

Section: Biomedical Applications Of Heart-on-a-chipmentioning

confidence: 99%

Fabrication and Biomedical Applications of Heart-on-a-chip

Yang

Xiao

et al. 2024

IJB

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Heart diseases have become the main killer threatening human health, and various methods have been developed to study heart disease. Among them, heart-on-a-chip has emerged in recent years as a method for constructing disease (or normal) models in vitro and is considered as a promising tool to study heart diseases. Compared with other methods, the advantages of heart-on-a-chip include the high portability, high throughput, and the capability to mimic microenvironments in vivo. It has shown a great potential in disease mechanism study and drug screening. In this paper, we review the recent advances in heart on-a-chip, including the fabrication methods (e.g., 3D bioprinting) and biomedical applications. By analyzing the structure of the existing heart-on-a-chip, we proposed that a highly integrated heart-on-a-chip includes four elements: Microfluidic chips, cells/microtissues, microactuators to construct the microenvironment, and microsensors for results readout. Finally, the current challenges and future directions of heart-on-a-chip are discussed.

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“…Ren et al. 153 reported a “heart-on-a-chip” platform that combines microgrooves and electrical pulse stimulation to recapitulate the well-aligned structure and synchronous beating of cardiomyocytes, which could provide a high-throughput drug screening platform for preclinical drug development. A bioartificial liver consisting of a surface-engineered microfluidic silicon chip with microtrenches mimicking hepatic sinusoids has also been reported 154 .…”

Section: Application Of Biotechnology In Pharmaceutical Products Anal...mentioning

confidence: 99%

Emerging biotechnology applications in natural product and synthetic pharmaceutical analyses

Chen

Zhang

et al. 2022

Acta Pharmaceutica Sinica B

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Combined Effects of Electric Stimulation and Microgrooves in Cardiac Tissue‐on‐a‐Chip for Drug Screening

Cited by 24 publications

References 49 publications

Multiscale Anisotropic Scaffold Integrating 3D Printing and Electrospinning Techniques as a Heart‐on‐a‐Chip Platform for Evaluating Drug‐Induced Cardiotoxicity

Multiscale Anisotropic Scaffold Integrating 3D Printing and Electrospinning Techniques as a Heart‐on‐a‐Chip Platform for Evaluating Drug‐Induced Cardiotoxicity

Fabrication and Biomedical Applications of Heart-on-a-chip

Emerging biotechnology applications in natural product and synthetic pharmaceutical analyses

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