“…Up to now, different agents such as acid, alkali, reducing agents, and enzymes were used for pectin extraction under the condition of traditional hot solution, microwave-assisted, ultrasonic-assisted heating, high hydrostatic pressure treatment, subcritical water and other methods (Ma et al, 2013;Guo et al, 2014;Methacanon et al, 2014;Seixas et al, 2014;Wang et al, 2014). All the variables obviously affected the structural features and functional properties of pectin (Li et al, 2015). Among them, subcritical water was proved to be more effective for extraction of pectin (Wang et al, 2014) and nonpolar compounds such as protein (Xia et al, 2012) from plant materials, as well as pectic substances which are complexed with Ca 2+ (and Mg 2+ ) in cell walls and therefore require the action of a chelating agent, such as sodium hexametaphosphate (Yamaguchi et al, 1996).…”