Combined Effects of Probucol and Bezafibrate on Lipoprotein Metabolism and Liver Cholesteryl Ester Transfer Protein mRNA in Cholesterol-Fed Rabbits

Ou, Jiafu; Saku, Keijiro; Jimi, Shiro; Liao, Yuan-Lan; Ohta, Takao; Zhang, Bo; Arakawa, Kikuo

doi:10.1253/jcj.63.471

Cited by 3 publications

(3 citation statements)

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“…In the JW rabbits of the present study, LDL, IDL, VLDL and chylomicron cholesterol concentrations were increased 22‐, 77‐, 59‐ and 41‐fold, respectively, after 16 weeks on the high‐fat diet. In the New Zealand or JW rabbit, lipoprotein cholesterol concentrations increased to similar extents (Daley et al ., ; Ou et al ., ). Yoshida et al .…”

Section: Discussionmentioning

confidence: 97%

“…New Zealand rabbits are popular and may be induced to develop hyperlipidemia by administering a high‐cholesterol diet (Van Heek and Zilversmit, ; Daley et al ., ; Rajamannan et al ., ). Similarly, Japanese White (JW) rabbits have been exploited in studies of fat tolerance (Ou et al ., ) and have been compared with WHHL rabbits (Suzuki et al ., ). WHHL rabbits have been further used to examine the protective effects of lipid‐lowering agents on atherosclerosis (Ou et al ., ; Suzuki et al ., ; Rajamannan et al ., ; Yin et al ., ).…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, Japanese White (JW) rabbits have been exploited in studies of fat tolerance (Ou et al ., ) and have been compared with WHHL rabbits (Suzuki et al ., ). WHHL rabbits have been further used to examine the protective effects of lipid‐lowering agents on atherosclerosis (Ou et al ., ; Suzuki et al ., ; Rajamannan et al ., ; Yin et al ., ). To measure plasma concentrations of lipoproteins, researchers often use ultracentrifugation, which is a cumbersome and time‐consuming procedure requiring relatively large sample volumes (0.5–2.0 mL; Shames et al ., ; Van Heek and Zilversmit, ; Fan et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Anion‐exchange HPLC separation of five major rabbit lipoproteins using a nonporous diethylaminoethyl‐ligated gel with a perchlorate‐containing eluent

Hirowatari

Kon

Shimura

et al. 2011

Biomedical Chromatography

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Rabbits are often used as experimental animals in studies of atherosclerosis and hyperlipidemia. Although rabbit lipoproteins can be quantitated by sequential ultracentrifugation, a simpler and more reproducible method is desirable for detailed analyses. The current study describes a method to analyze rabbit lipoproteins in plasma by anion-exchange high-performance liquid chromatography using a column filled with nonporous, diethylaminoethyl-ligated polymers. A solution of NaClO₄ was used to adjust the ionic strength of the eluent. The method required only 15 μL of plasma and analysis was completed in 23 min. Five lipoprotein fractions (high-density lipoprotein, low-density lipoprotein, intermediate-density lipoprotein, very-low-density lipoprotein and chylomicrons) were eluted with step-wise increases in a concentration of NaClO₄. The post-column eluate was reacted with an enzymatic reagent to determine total cholesterol, and the lipoprotein-cholesterol fraction was calculated according to relative peak areas in the chromatogram. The within-day and between-day assay coefficients of variation for lipoprotein cholesterol levels ranged between 0.436 and 7.143% and between 2.905 and 10.526%, respectively. Administering a high-fat diet increased lipoprotein-cholesterol concentrations by 6- to 77-fold. The method described here was nevertheless able to quantitate levels of lipoprotein-cholesterol in plasma samples from these rabbits. These results indicate that this method may be applied to lipoprotein studies using hyperlipidemic rabbit models.

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Section: Discussionmentioning

confidence: 97%