Combined effects of ration levels and temperature on immune responses of the triangle sail mussel Hyriopsis cumingii

Abstract

Nowadays, the cultivation mode of Hyriopsis cumingii urgently needs transformation and upgrading, and realizing sustainable aquaculture lacks the selection of suitable algae and the exploration of culture conditions. This experiment is to evaluate combined effects of ration levels (low ration, medium ration and high ration) and temperature (13.0 ± 0.2°C, 23.0 ± 0.2°C, and 33.0 ± 0.2°C) on immune responses of H. cumingii. Activities of immune enzymes (alkaline phosphatase, superoxide dismutase, catalase and lysozyme) and expression of immune-related genes (interleukin-17 and inhibitor of apoptosis protein) in mussels were analysed during 21 days exposure period. Results suggested that the diatom Navicula pelliculosa was suitable algae for H. cumingii, the optimal ration level and temperature of H. cumingii were the medium ration and 23℃. Semi-starvation, excessive dietary, low-or high-temperature stimulated immune activities, and when beyond the immune regulation range, most immune enzyme activities decreased. Acute stress also stimulated expression of HcIL-17 and IAP, and long-term stress of discomfort temperature and ration levels led to immune dysfunction and affected expression of these genes. The study has practical significance in realizing the sustainable aquaculture of H. cumingii and provides a broader perspective regarding the water ecosystem.

Enzyme activities and gene expression in digestion and metabolism of Hyriopsis cumingii under different temperature and diet ration

“…During the acclimatization period, mussels were fed with N. pelliculosa at biomass 4 × 10 4 cells mL − 1 ca. 12.5 mg L − 1 , which was according to results of the preliminary experiment (Sun et al, 2021).…”

“…Therefore, the present study detected above biochemical parameters in H. cumingii exposed to different ration levels and temperatures, providing insight into metabolic performance, growth and physiological status. Our previous research found that uncomfortable food and temperature levels increased activities of immune enzymes and expression of immune genes in a short time, but when it exceeded the immune regulatory range, it caused immune damage of H. cumingii (Sun et al, 2021). We hypothesized that different ration levels and temperatures also affects the digestive and metabolic physiology of H. cumingii.…”

Sex-specific digestive performance of mussels exposed to warming and starvation

“…Lysozyme is a typical humoral defense component, a large number of experiments have studied its role and mechanism in the immune response of marine bivalves (Muroga and Takahashi, 2007;Hu et al, 2015;Sun et al, 2022). The action mechanism of lysozyme mainly lies in its ability to destroy peptidoglycan components in dissolved bacterial cell wall, thus causing bacterial cell wall damage and disintegration (Liu et al, 2003).…”

Characterization of Gut Microbiome in the Mud Snail Cipangopaludina cathayensis in Response to High-Temperature Stress

“…Indeed, global warming poses a serious threat to global aquaculture production [ 4 ]. Rising water temperature has a series of deleterious effects on the immunity [ 2 , 5 ], metabolism [ 6 , 7 ], reproduction and development [ 8 , 9 ], and behavioral patterns [ 10 , 11 ] of aquatic organisms.…”

“…Freshwater ecosystems are particularly fragile under the scenario of global change [ 17 ]. Previous studies have found that high-temperature stress has many adverse effects on freshwater mollusks [ 5 , 18 , 19 , 20 , 21 ] and is a key factor that causes mass mortality [ 21 ]. To date, few studies have focused on the adverse impacts of thermal stress on C. cathayensis [ 22 ].…”