With the rapid development of the construction industry, an increasing amount of attention were paid by universities to the development of civil engineering experiment courses so as to improve the practical research abilities of students. In recent years, due to the frequent occurrence of civil engineering laboratory accidents, it has become an urgent issue regarding on what factors influencing safety risks and how to assess and reduce the safety risks in civil engineering laboratories. Based on the lab criticity index (LCI) model, the research specificities of civil engineering laboratories were analyzed through literature review and expert interviews and 13 risk factors of civil engineering laboratories, from the four aspects of man, object, management, and environment, identified. The data for each parameter in the LCI model was obtained through a questionnaire survey, and finally the LCI value was calculated to evaluate priority. Among them, insufficient safety awareness of operators, danger due to equipment failure, imperfect management policies, and complex floor conditions were listed as the most common risk factors. Based on the LCI model, the worsening factors of these four risk factors were further analyzed. The LCI model is applied to the new research field of safety risk assessment in civil engineering laboratories that few researchers have studied before and a risk list for civil engineering laboratories was created. We revealed the safety status of civil engineering laboratories in Jiangsu Province and provided feasible suggestions for improving the management and supervision of civil engineering laboratories at universities. It can strengthen operator awareness of the risks in civil engineering laboratories and improve the social group’s attention to the safety risks of the laboratories, thus reducing the accidents’ possibility and seriousness of civil engineering laboratories.