Combined effects of temperature and copper on oxygen consumption and antioxidant responses in the mudflat fiddler crab Minuca rapax (Brachyura, Ocypodidae)

Capparelli, Mariana V.; Bordon, Isabella C.; Gusso-Choueri, Paloma Kachel; Abessa, Denis Moledo de Souza; McNamara, John Campbell

doi:10.1016/j.cbpc.2019.05.005

Cited by 12 publications

(5 citation statements)

References 49 publications

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“…Cu is a necessary trace element for the growth and development of all known organisms, including humans and other vertebrates; it mainly acts as a cofactor for various enzymes involved in protecting cells against destruction by oxidation 4 . In crustaceans, copper is a natural component of hemocyanin, the respiratory pigment used in oxygen transport and also plays a role in the molting cycle and in the synthesis of metallothioneins 5 . Owing to the human intervention and industrial activities, Cu discharged from metal mines, smelters, and municipal sewage finds its way into aquatic systems, leading to elevated concentrations in freshwater 6 .…”

Section: Introductionmentioning

confidence: 99%

Acute and sub-chronic effects of copper on survival, respiratory metabolism, and metal accumulation in Cambaroides dauricus

Bao

Xing

Feng

et al. 2020

Sci Rep

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Trace metal contamination in the aquatic ecosystem occurs worldwide: although copper is an essential trace metal, it is considered as a pollutant at certain levels in China. Freshwater crayfish Cambaroides dauricus is a commercially important wild species in northeastern China, in which is an important heavy industry area. The population of C. dauricus was decreasing sharply due to the environmental pollution and human intervention over the past 20 years. However, nothing is known regarding the responses of this species to trace metal toxicants. This study aimed to determine the acute and chronic toxicity of Cu and its toxicological effects on respiratory metabolism, as well as Cu accumulation in C. dauricus. The acute (96 h) median lethal concentration (LC50) value of 32.5 mg/L was detected in C. dauricus. Then, acute (96 h; 8.24, 16.48 mg/L) and sub-chronic (14 days; 2.06, 4.12 mg/L) exposure in Cu was investigated by estimating the oxygen consumption rate, ammonium excretion rate, and Cu accumulation. Both acute and sub-chronic Cu exposure induced an inhibition of the oxygen consumption rate and ammonium excretion rate, and thereby, an increased O:N ratio. The shift in O:N ratio indicated a metabolic substrate shift towards lipid and carbohydrate metabolism under Cu stress. Cu accumulation in the hepatopancreas and muscles throughout the study was found to be time-dependent and concentration-dependent. The maximum accumulation in the hepatopancreas and muscle were almost 31.6 folds of the control after 14 days’ exposure to 4.12 mg/L concentration. Based on the present work, we suggest that crayfish be considered a potential bioindicator of environmental pollution in freshwater systems. The study provides basic information for further understanding of the toxicological responses of this species to trace metals.

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Section: Introductionmentioning

confidence: 99%

Acute and sub-chronic effects of copper on survival, respiratory metabolism, and metal accumulation in Cambaroides dauricus

Bao

Xing

Feng

et al. 2020

Sci Rep

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“…Our study reveals that fiddler crabs are sensitive to human disturbance and they modify their behaviour in areas affected by human activities. Previous studies have employed fiddler crabs as bioindicator species to assess metal contamination (Capparelli et al 2016(Capparelli et al , 2017(Capparelli et al , 2019Yáñez-Rivera et al 2019), microplastic and organophosphate pesticide contamination (Villegas et al 2021), and rare-earth elements (Lavezzo et al 2020). To the best of our knowledge, the use of fiddler crab behaviour as a bioindicator of human disturbance in coastal regions has not been explored extensively.…”

Section: Discussionmentioning

confidence: 99%

Human disturbance affects behavioural time allocation in a fiddler crab (<em>Austruca annulipes</em>) in Southern Thailand

Chumsri,

Tina,

Vongkok

et al. 2023

J. Anim. Behav. Biometeorol.

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Human disturbances affect the macroinvertebrate populations in coastal regions. They respond to disturbances by altering their density and behaviour. Therefore, many of them are used as bioindicator species of human disturbance. Here, we pioneer the use of fiddler crabs to examine whether they alter their behaviour under human disturbance. Male fiddler crabs possess one large claw used for courting (waving) and fighting, and one small feeding claw, whereas females have two feeding claws. They show several surface activities. This study investigates (1) the effects of human disturbance on density and sex-ratio, and (2) the effects of human disturbance, and sex on behavioural time allocations in Austruca annulipes. Their density, sex-ratio, and time allocations were investigated in human-disturbed area (DA) and nondisturbed area (NDA). They showed feeding, feeding and walking, walking, running, standing/vigilance, inside burrows, burrowing, grooming, fighting, and waving. The results showed that crab density was higher and the sex ratio was more male biased in NDA than in DA. Human disturbance and sex affected time allocations but their interaction had no effect. Crabs in DA spent more time running, standing, and inside burrows but less time walking, burrowing, fighting, and waving than crabs in NDA. Between sexes, males spent more time standing, burrowing, grooming, and fighting but less time feeding, and walking than females. This indicates that human disturbances force the crabs to spend more time on anti-predator and escape behavior (standing/vigilance, running, inside burrows) rather than courting (waving) and constructing burrows (mating/breeding sites), which are important for breeding.

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“…For example, Cu 2+ exposure enhances the activities of antioxidative enzymes such as SOD and glutathione peroxidase (Gpx) in hepatopancreas of L. vannamei [ 37 ] and Callinectes sapidus [ 38 ], and gills of O. niloticus [ 39 ], reflecting an occurrence of oxidative stress. In contrast, exposure to high levels of waterborne Cu 2+ decreases enzymatic and non-enzymatic antioxidants and induces oxidative damage in the gills of P. clarkia [ 40 ], the hepatopancreas of Minuca rapax [ 41 ], and the brain of Cyprinus carpio [ 42 ]. Our study further showed that the antioxidant capacity in different tissues of E. sinensis decreased following exposure to 0.70 mg/L of Cu 2+ , indicating that a higher level of Cu 2+ exposure induces oxidative damage.…”

Section: Discussionmentioning

confidence: 99%

“…Moderate salinity levels increased GST activity to alleviate the lethal toxicity of Cu 2+ , but high salinity levels worsen the Cu 2+ -induced oxidative damage in Danio rerio embryos [ 50 ]. In M. rapax, the higher temperature (35 °C) significantly increased Cu 2+ -induced oxidative stress [ 41 ]. Carvalho et al (2015) suggested that the effect of Cu 2+ on the response of antioxidant defense systems was determined by water pH in Prochilodus lineatus [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Copper Exposure on Oxidative Stress, Apoptosis, Endoplasmic Reticulum Stress, Autophagy and Immune Response in Different Tissues of Chinese Mitten Crab (Eriocheir sinensis)

Feng

Song

et al. 2022

Antioxidants

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High concentrations of copper (Cu2+) pose a great threat to aquatic animals. However, the mechanisms underlying the response of crustaceans to Cu2+ exposure have not been well studied. Therefore, we investigated the alterations of physiological and molecular parameters in Chinese mitten crab (Eriocheir sinensis) after Cu2+ exposure. The crabs were exposed to 0 (control), 0.04, 0.18, and 0.70 mg/L of Cu2+ for 5 days, and the hemolymph, hepatopancreas, gills, and muscle were sampled. The results showed that Cu2+ exposure decreased the antioxidative capacity and promoted lipid peroxidation in different tissues. Apoptosis was induced by Cu2+ exposure, and this activation was associated with the mitochondrial and ERK pathways in the hepatopancreas. ER stress-related genes were upregulated in the hepatopancreas but downregulated in the gills at higher doses of Cu2+. Autophagy was considerably influenced by Cu2+ exposure, as evidenced by the upregulation of autophagy-related genes in the hepatopancreas and gills. Cu2+ exposure also caused an immune response in different tissues, especially the hepatopancreas, where the TLR2-MyD88-NF-κB pathway was initiated to mediate the inflammatory response. Overall, our results suggest that Cu2+ exposure induces oxidative stress, ER stress, apoptosis, autophagy, and immune response in E. sinensis, and the toxicity may be implicated following the activation of the ERK, AMPK, and TLR2-MyD88-NF-κB pathways.

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Combined effects of temperature and copper on oxygen consumption and antioxidant responses in the mudflat fiddler crab Minuca rapax (Brachyura, Ocypodidae)

Cited by 12 publications

References 49 publications

Acute and sub-chronic effects of copper on survival, respiratory metabolism, and metal accumulation in Cambaroides dauricus

Acute and sub-chronic effects of copper on survival, respiratory metabolism, and metal accumulation in Cambaroides dauricus

Human disturbance affects behavioural time allocation in a fiddler crab (<em>Austruca annulipes</em>) in Southern Thailand

Effects of Copper Exposure on Oxidative Stress, Apoptosis, Endoplasmic Reticulum Stress, Autophagy and Immune Response in Different Tissues of Chinese Mitten Crab (Eriocheir sinensis)

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