Combined expression of chitinase and β-1,3-glucanase genes in indica rice (Oryza sativa L.) enhances resistance against Rhizoctonia solani

Sridevi, G.; Parameswari, C.; Sabapathi, N.; Raghupathy, Vengoji; Veluthambi, K.

doi:10.1016/j.plantsci.2008.04.011

Cited by 97 publications

(64 citation statements)

References 38 publications

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“…Therefore, chitinase might be a defense gene involved in basal resistance of Sunny plants to the pathogen. This finding is in agreement with the observations of Sridevi et al [26] on the role of chitinase in resistance of rice plants to R. solani. Similarly in tomato, investigations of Chen and associates [25] revealed the involvement of chitinase in defense of the plants against Botrytis cinerea as a destructive phytopathogen with necrotrophic lifestyle.…”

Section: Discussionsupporting

confidence: 94%

“…Several reports likewise suggest that priming of peroxidase transcripts or activities in various plants which contributes to phenolics-mediated resistance against necrotrophic phytopathogens [10,24]. Chitinase gene could activate the expression of pathogenesis-related (PR) genes, subsequently enhancing transgenic plant resistance to necrotrophic pathogens [25,26]. In the present study, a correlation was observed between higher level of resistance in Sunny plants and earlier upregulation of the chitinase gene (LOC544149) in this cultivar at 12 hpi.…”

Section: Discussionsupporting

confidence: 59%

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The Role of Pathogenesis-Related Proteins in the Tomato-Rhizoctonia solani Interaction

Taheri

Tarighi

2012

Journal of Botany

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Rhizoctonia solani is one of the most destructive pathogens causing foot rot disease on tomato. In this study, the molecular and cellular changes of a partially resistant (Sunny 6066) and a susceptible (Rio Grande) tomato cultivar after infection with necrotrophic soil-borne fungus R. solani were compared. The expression of defense-related genes such as chitinase (LOC544149) and peroxidase (CEVI-1) in infected tomato cultivars was investigated using semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). This method revealed elevated levels of expression for both genes in the partially resistant cultivar compared to the susceptible cultivar. One of the most prominent facets of basal plant defense responses is the formation of physical barriers at sites of attempted fungal penetration. These structures are produced around the sites of potential pathogen ingress to prevent pathogen progress in plant tissues. We investigated formation of lignin, as one of the most important structural barriers affecting plant resistance, using thioglycolic acid assay. A correlation was found between lignification and higher level of resistance in Sunny 6066 compared to Rio Grande cultivar. These findings suggest the involvement of chitinase, peroxidase, and lignin formation in defense responses of tomato plants against R. solani as a destructive pathogen.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 59%

The Role of Pathogenesis-Related Proteins in the Tomato-Rhizoctonia solani Interaction

Taheri

Tarighi

2012

Journal of Botany

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“…Transgenic rice plants of local cultivars developed through Agrobacterium-mediated transformation expressing rice chitinase gene RC7 exhibit an enhanced resistance (up to 33.3 %) to R. solani under glasshouse conditions (Nandakumar et al 2007). Sridevi et al (2008) used Agrobacterium-mediated transformation for simultaneous introduction of chi11 gene and tobacco β-1,3-glucanase gene into cv. PB1.…”

Section: Introductionmentioning

confidence: 99%

Stability of sheath blight resistance in transgenic ASD16 rice lines expressing a rice chi11 gene encoding chitinase

Rajesh¹,

Maruthasalam²,

Kalpana³

et al. 2016

Biologia plant.

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Development of transgenic plants by introducing defense genes is one of the strategies to engineer disease resistance. Transgenic ASD16 rice plants harbouring rice chitinase chi11 gene, belonging to a PR-3 group of defense gene conferring sheath blight (Rhizoctonia solani Kuhn) resistance, were used in this study. Three T 2 homozygous lines (ASD16-4-1-1, 5-1-1, and 6-1-1) were identified from seven putative (T 0 ) transgenic lines expressing chi11 using Western blotting analysis. The inheritance of sheath blight resistance in those lines was studied over generations. The stability of chi11 expression up to T 4 generation in all the three homozygous lines was proved by Western blot and the stability of sheath blight resistance in the homozygous lines was proved up to T 4 generation using detached leaf and intact leaf sheath assays. Among the three homozygous lines tested, ASD16-4-1-1 showed consistent results in all the generations and gave a better protection against the sheath blight pathogen than the other two lines.

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“…Transgenic groundnut overexpressing a tobacco β-1,3-glucanase has enhanced resistance against two major fungal pathogens, Cercospora arachidicola and Aspergillus flavus (Sundaresha et al 2010). Transgenic plants expressing glucanase, in combination with other defense-related genes, have also been reported to be resistant against fungal pathogens (Mackintosh et al 2006;Sridevi et al 2008;Amian et al 2011). Zhu et al (1994) expressed alfalfa acidic glucanase gene singly and in conjunction with chitinase gene in tobacco for resistance against Cercospora nicotianae .…”

Section: Introductionmentioning

confidence: 99%

Increased resistance to fungal wilts in transgenic eggplant expressing alfalfa glucanase gene

Singh

Ambroise

Haïcour

et al. 2014

Physiol Mol Biol Plants

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The wilt diseases caused by Verticillium dahliae and Fusarium oxysporum are the major diseases of eggplant (Solanum melongena L.). In order to generate transgenic resistance against the wilt diseases, Agrobacteriummediated gene transfer was performed to introduce alfalfa glucanase gene encoding an acidic glucanase into eggplant using neomycin phosphotransferase (npt-II) gene as a plant selection marker. The transgene integration into eggplant genome was confirmed by Polymerase chain reaction (PCR) and Southern blot analysis and transgene expression by the glucanase activity and western blot analysis. The selected transgenic lines were challenged with V. dahliae and F. oxysporum under in vitro and in vivo growth conditions, and transgenic lines showed enhanced resistance against the wilt-causing fungi with a delay of 5-7 days in the disease development as compared to wild-type plants.

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Combined expression of chitinase and β-1,3-glucanase genes in indica rice (Oryza sativa L.) enhances resistance against Rhizoctonia solani

Cited by 97 publications

References 38 publications

The Role of Pathogenesis-Related Proteins in the Tomato-Rhizoctonia solani Interaction

The Role of Pathogenesis-Related Proteins in the Tomato-Rhizoctonia solani Interaction

Stability of sheath blight resistance in transgenic ASD16 rice lines expressing a rice chi11 gene encoding chitinase

Increased resistance to fungal wilts in transgenic eggplant expressing alfalfa glucanase gene

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