Combined extract of Moringa oleifera and Centella asiatica modulates oxidative stress and senescence in hydrogen peroxide-induced human dermal fibroblasts

Hisam, Elly Ezlinda Abdul; Rofiee, Mohd Salleh; Khalid, Aina Mardhia; Jalaluddin, Ahmad Firdaus; Yusof, Mat Ikram; Idris, Mushrifah; Ramli, Salfarina; James, Richard Johari; Yoeng, Wee Jack; Kek, Teh Lay; Salleh, Mohd Zaki

doi:10.3906/biy-1708-23

Cited by 18 publications

(10 citation statements)

References 37 publications

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“…Moreover, our evidence suggests that MOe may prevent DEHP-induced mitochondrial damage by stabilizing membrane potential and decreasing caspase activation in the mitochondrion. A recent paper demonstrated that pretreating human neuron cells with MOe showed a significant resistance to H 2 O 2 -induced apoptotic cell death [ 88 ].…”

Section: Discussionmentioning

confidence: 99%

Moringa oleifera Protects SH-SY5YCells from DEHP-Induced Endoplasmic Reticulum Stress and Apoptosis

et al. 2021

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Moringa oleifera (MO) is a medicinal plant that has been shown to possess antioxidant, anticarcinogenic and antibiotic activities. In a rat model, MO extract (MOe) has been shown to have a protective effect against brain damage and memory decline. As an extending study, here, we have examined the protective effect of MOe against oxidative stress and apoptosis caused in human neuroblastome (SH-SY5Y) cells by di-(2-ethylhexyl) phthalate (DEHP), a plasticizer known to induce neurotoxicity. Our data show that MOe prevents oxidative damage by lowering reactive oxygen species (ROS) formation, restoring mitochondrial respiratory chain complex activities, and, in addition, by modulating the expression of vitagenes, i.e., antioxidant proteins Nrf2 and HO-1. Moreover, MOe prevented neuronal damage by partly inhibiting endoplasmic reticulum (ER) stress response, as indicated by decreased expression of CCAAT-enhancer-binding protein homologous protein (CHOP) and Glucose-regulated protein 78 (GRP78) proteins. MOe also protected SH-SY5Y cells from DEHP-induced apoptosis, preserving mitochondrial membrane permeability and caspase-3 activation. Our findings provide insight into understanding of molecular mechanisms involved in neuroprotective effects by MOe against DEHP damage.

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Section: Discussionmentioning

confidence: 99%

Moringa oleifera Protects SH-SY5YCells from DEHP-Induced Endoplasmic Reticulum Stress and Apoptosis

et al. 2021

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“…For establishment of the premature senescence model, hDFs were treated with 50, 100, 200 and 400 μ M H 2 O 2 (in 10% FBS in high glucose DMEM) at 37°C with 5% CO 2 for 1 or 2 h. Cells were then washed with serum-free high glucose DMEM twice to remove residual H 2 O 2 (20,21) and cultured in high glucose DMEM with 10% FBS for 4 days; growth medium changed every 48 h. In the CdM treatment group, H 2 O 2 -treated cells (200 μ M, 1 h) were washed with serum free medium and incubated with CdM for 4 days. To ensure sufficient nutrients were available, CdM was changed every 24 h.…”

Section: Methodsmentioning

confidence: 99%

Conditioned medium derived from human amniotic stem cells delays H2O2‑induced premature senescence in human dermal fibroblasts

et al. 2019

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Stem cells derived from human amniotic membrane (hAM) are promising targets in regenerative medicine. A previous study focused on human amniotic stem cells in skin wound and scar-free healing. The present study aimed to investigate whether hydrogen peroxide (H2O2)-induced senescence of human dermal fibroblasts (hDFs) was influenced by the anti-aging effect of conditioned medium (CdM) derived from human amniotic stem cells. First, the biological function of two types of amniotic stem cells, namely human amniotic epithelial cells (hAECs) and human amniotic mesenchymal stem cells (hAMSCs), on hDFs was compared. The results of cell proliferation and wound healing assays showed that CdM promoted cell proliferation and migration. In addition, CdM from hAECs and hAMSCs significantly promoted proliferation of senescent hDFs induced by H2O2. These results indicated that CdM protects cells from damage caused by H2O2. Treatment with CdM decreased senescence-associated β-galactosidase activity and improved the entry of proliferating cells into the S phase. Simultaneously, it was found that CdM increased the activity of superoxide dismutase and catalase and decreased malondialdehyde by reducing H2O2-induced intracellular reactive oxygen species production. It was found that CdM downregulated H2O2-stimulated 8-hydroxydeoxy-guanosine and γ-H2AX levels and decreased the expression of the senescence-associated proteins p21 and p16. In conclusion, the findings indicated that the paracrine effects derived from human amniotic stem cells aided delaying oxidative stress-induced premature senescence.

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“…The colour intensity was graded as either very high (++++), high (+++), moderate (++), low (+), or not detected (−) compared to the positive control. The quantitative results were determined as previously described by Hisam et al with slight modification [28]. The absorbance (Wavelength range: 435– 635 nm) was measured by using an ELISA plate reader (Tecan 200, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

The cytotoxic effect and glucose uptake modulation of Baeckea frutescens on breast cancer cells

Shahruzaman

Mustafa

Ramli³

et al. 2019

BMC Complement Altern Med

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Background Baeckea frutescens ( B. frutescens ) of the family Myrtaceae is a plant that has been used in traditional medicine. It is known to have antibacterial, antipyretic and cytoprotective properties. The objective of this study is to explore the mechanism of B. frutescens leaves extracts in eliminating breast cancer cells. Method B. frutescens leaves extracts were prepared using Soxhlet apparatus with solvents of different polarity. The selective cytotoxicity of these extracts at various concentrations (20 to 160 μg/ml) were tested using cell viability assay after 24, 48 and 72 h of treatment. The IC 50 value in human breast cancer (MCF-7 and MDA-MB-231) and mammary breast (MCF10A) cell lines were determined. Apoptotic study using AO/PI double staining was performed using fluorescent microscope. The glucose uptake was measured using 2-NBDG, a fluorescent glucose analogue. The phytochemical screening was performed for alkaloids, flavonoids, tannins, triterpenoids, and phenols. Results B. frutescens leaves extracts showed IC 50 value ranging from 10 -127μg/ml in MCF-7 cells after 72 h of treatment. Hexane extract had the lowest IC 50 value (10μg/ml), indicating its potent selective cytotoxic activity. Morphology of MCF-7 cells after treatment with B. frutescens extracts exhibited evidence of apoptosis that included membrane blebbing and chromatin condensation. In the glucose uptake assay, B. frutescens extracts suppressed glucose uptake in cancer cells as early as 24 h upon treatment. The inhibition was significantly lower compared to the positive control WZB117 at their respective IC 50 value after 72 h incubation. It was also shown that the glucose inhibition is selective towards cancer cells compared to normal cells. The phytochemical analysis of the extract using hexane as the solvent in particular gave similar quantities of tannin, triterpenoids, flavonoid and phenols. Presumably, these metabolites have a synergistic effect in the in vitro testing, producing the potent IC 50 value and subsequently cell death. Conclusion This study reports the potent selective cytotoxic effect of B. frutescens leaves hexane extract against MCF-7 cancer cells. B. frutescens extracts selectively suppressed cancer cells glucose uptake and subsequently induced cancer cell death. These findings suggest a new role of B. frutescens in cancer cell metabolism. Electronic supplementary material The online version of this article (10.1186/s12906-019-2628-z) contains supplementary material, which is available to authorized users.

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Combined extract of Moringa oleifera and Centella asiatica modulates oxidative stress and senescence in hydrogen peroxide-induced human dermal fibroblasts

Cited by 18 publications

References 37 publications

Moringa oleifera Protects SH-SY5YCells from DEHP-Induced Endoplasmic Reticulum Stress and Apoptosis

Moringa oleifera Protects SH-SY5YCells from DEHP-Induced Endoplasmic Reticulum Stress and Apoptosis

Conditioned medium derived from human amniotic stem cells delays H2O2‑induced premature senescence in human dermal fibroblasts

The cytotoxic effect and glucose uptake modulation of Baeckea frutescens on breast cancer cells

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