Combined hepatotoxicity of imidacloprid and microplastics in adult zebrafish: Endpoints at gene transcription

Luo, Ting; Weng, You; Huang, Zhuizui; Zhao, Yao; Jin, Yuanxiang

doi:10.1016/j.cbpc.2021.109043

Cited by 31 publications

(11 citation statements)

References 44 publications

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“…This suggested that MPs activated immune mechanisms in yellow catfish, which was due to the activation of the NF-κB signaling pathway, leading to the increased expression of related signaling factors (Wu et al, 2020;Yang et al, 2020). The same result was also obtained by Luo et al (2021) who studied zebrafish (Danio rerio). Compared with normoxia, the expression of TNF-α was higher under hypoxia with 0 MPs, although this increase was not significant.…”

Section: Discussionsupporting

confidence: 65%

“…What is more, MPs not only affect the expression of IFN at the gene level but also increase the secretion of the protein corresponding to IFN (Jin et al, 2018). Luo et al (2021) found that MPs induced the expression of increased IL-8 compared with the control group. The expression changes of these proinflammatory biomarkers under the MPs make them become the important index on the evaluation of fish welfare.…”

Section: Introductionmentioning

confidence: 90%

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Effects of Microplastics on Immune Responses of the Yellow Catfish Pelteobagrus fulvidraco Under Hypoxia

Jiang

et al. 2021

Front. Physiol.

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Compared with marine organisms, research on microplastics (MPs) in freshwater organisms is still less although MPs have been widely found in the freshwater ecosystem. Hypoxia is a ubiquitous issue in freshwater aquaculture, and under such scenarios, the toxic effects of MPs on typical aquaculture fish need to be clarified. In this study, we studied the effects of MPs (polystyrene) on specific growth rate (SGR), hypoxia-inducible factor-1α (HIF-1α), tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8), and interferon (IFN) in the yellow catfish (Pelteobagrus fulvidraco) under hypoxic conditions. After 15 days of exposure, the SGR was not affected by MPs or hypoxia. MPs significantly increased the expressions of HIF-1α and TNF-α but inhibited the expression of IFN at high concentration MPs under normoxia. However, hypoxia significantly inhibited the expression of IL-8 and TNF-α under high MP concentration and low MP concentration, respectively. In addition, MPs had significant concentration-dependent inhibitory effects on IFN under hypoxia. Surprisingly, a positive correction between HIF-1α and TNF-α was found in fish. Although hypoxia might alleviate the effects of MPs with low concentrations, the interaction of hypoxia and MPs aggravated the negative effects of MPs on immune factors at high concentration MPs. This study provided new insight into the complex effects of hypoxia and MPs on aquatic organisms, and future studies should focus on the cellular pathways of immune cells in fish. Given that MPs could induce the immune response in fish, considerations should be paid to the impacts of MPs on freshwater aquaculture, and hypoxia should be taken into consideration when evaluating the effects of MPs.

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Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 90%

Effects of Microplastics on Immune Responses of the Yellow Catfish Pelteobagrus fulvidraco Under Hypoxia

Jiang

et al. 2021

Front. Physiol.

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“…Oxyfluorfen damaged the lipid and sugar metabolism in the liver of zebrafish larvae, and the liver injury caused by metabolic dysfunction eventually affected the early development of zebrafish (Li et al, 2021). The combined hepatotoxicity in imidacloprid and microplastics caused the changes of glucose and lipid metabolism and the expression of inflammation-related genes in zebrafish (Luo et al, 2021). Therefore, the hepatotoxicity of pollutants to zebrafish should be paid attention to.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals hepatotoxicity in zebrafish induced by cyhalofop-butyl

Zhou

Yang

Liu

2022

Preprint

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Cyhalofop-butyl is a highly effective aryloxyphenoxypropionate herbicide and widely used for weed control in paddy fields. With the increasing residue of herbicides, it poses a threat to the survival of aquatic organisms. Here, we evaluated the effect of cyhalofop-butyl on zebrafish to explore its potential hepatotoxic mechanism. The results showed that cyhalofop-butyl (0.1, 0.2 and 0.4 mg/L) induced hepatocyte degeneration, vacuolation and necrosis of larvae after embryonic exposure for 4 days and caused liver atrophy after exposure for 5 days. Meanwhile, the activities of enzymes related to liver function named alanine transaminase (ALT), aspartate transaminase (AST) were significantly increased by 0.2 mg/L cyhalofop-butyl and higher. And the contents of triglyceride (TG) involved in lipid metabolism was significantly decreased by 0.4 mg/L cyhalofop-buty. The effects of cyhalofop-butyl on zebrafish larvae were further demonstrated by GO (Gene Ontology) and KEGG pathway analysis. Cyhalofop-butyl (0.1, 0.2, 0.4 mg/L) altered the expression of 116, 154, 397 genes in liver,these genes are mainly enriched in metabolism (such as lipid metabolism, amino acid metabolism), immune system (Toll-like receptor signaling pathway) and endocrine system (PPAR signaling pathway). Furthermore, the expression of key genes related to liver development combined with RNA-Seq results, indicated that cyhalofop-butyl might damage the liver development of zebrafish larvae and cause metabolic disorders. To sum up, our research results reveal the physiological and molecular responses of zebrafish liver to cyhalofop-butyl and provide new insights for further studying the mechanism of cyhalofop-butyl toxicity to aquatic organisms.Graphical AbstractHighlightsCyhalofop-butyl induced liver damage of zebrafish larvae and caused liver atrophyCyhalofop-butyl affected liver function by increasing the activities of ALT and AST.Cyhalofop-butyl disturbed the lipid metabolism of liver in zebrafish via inhibition TG levels.Cyhalofop-butyl exposure induced metabolism and immune system disturbance at transcriptional levels.

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“…The PS and IMI inhibited the growth of zebrafish, and altered the levels of glycolipid metabolism and oxidative stress-related biochemical parameters like super oxide dismutase (SOD) and catalase (CAT) activity. The IMI and PS alone and their combination significantly increased SOD and CAT activities in the liver of zebrafish, indicating an oxidative stress and inflammation in the test organism (Luo et al, 2021).…”

Section: Effects On Terrestrial Organisms and Nutrient Cyclingmentioning

confidence: 92%

“…for 21 days (Luo et al, 2021) (Table 2). The PS and IMI inhibited the growth of zebrafish, and altered the levels of glycolipid metabolism and oxidative stress-related biochemical parameters like super oxide dismutase (SOD) and catalase (CAT) activity.…”

Section: Effects On Terrestrial Organisms and Nutrient Cyclingmentioning

confidence: 99%

Challenges and opportunities in sustainable management of microplastics and nanoplastics in the environment

Sarkar

Dissanayake

Bolan

et al. 2022

Environmental Research

120

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Combined hepatotoxicity of imidacloprid and microplastics in adult zebrafish: Endpoints at gene transcription

Cited by 31 publications

References 44 publications

Effects of Microplastics on Immune Responses of the Yellow Catfish Pelteobagrus fulvidraco Under Hypoxia

Effects of Microplastics on Immune Responses of the Yellow Catfish Pelteobagrus fulvidraco Under Hypoxia

Transcriptome analysis reveals hepatotoxicity in zebrafish induced by cyhalofop-butyl

Challenges and opportunities in sustainable management of microplastics and nanoplastics in the environment

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