Combined identification of three miRNAs in serum as effective diagnostic biomarkers for HNSCC

Abstract

Background: Head and neck squamous cell carcinoma (HNSCC) is a disastrous disease with substantial morbidity and mortality. This study aims to explore the effective diagnostic and prognostic biomarkers for HNSCC.Methods: MiRNA expression data and corresponding clinical information of HNSCC from The Cancer Genome Atlas (TCGA) database were analyzed comprehensively to identify the miRNAs with diagnostic and prognostic power. The predictive ability of different classifications was analyzed for the three-miRNA combinations. Diagnostic and prognostic value were then evaluated and verified in clinical patients.Findings: 128 differentially expressed miRNAs in HNSCC tissues were identified in the TCGA dataset, and 10 miRNAs were finally selected for further study. Classification analysis developed a three-miRNA signature of hsa-mir-383, hsa-mir-615, and hsa-mir-877 with the best diagnosis power, which was verified in validation patients. Survival analysis indicated that different expression levels of hsa-mir-383, rather than that of hsa-mir-615 or hsa-mir-877 led to significantly different survival rates in both cohorts. Furthermore, the multivariate Cox hazards analysis suggested that the microRNA signature yielded statistical significance to predict clinical outcome independently from other clinical variables in validation patients.Interpretation: A three-miRNA signature of hsa-mir-383, hsa-mir-615, and hsa-mir-877 may serve as an excellent diagnostic biomarker for HNSCC, and potential prognostic significance for HNSCC patients.Funding: This work was supported by the grants of the (81901021), (2019GSF108277), China postdoctoral Scinence Foundation Grant (2019M652380), Fundamental Research Funds of Shandong University (2018CJ047).

High-Throughput RNA Sequencing Analysis of Plasma Samples Reveals Circulating microRNA Signatures with Biomarker Potential in Dengue Disease Progression

“…4D to F). Further, to understand if combined miRNA detection provided a better diagnostic power, the ROC analysis was performed for the three miRNAs together as described by Liu et al (19). Our analysis suggested that the combination of the miRNAs increased the AUC, 95% confidence interval (CI) value, and statistical significance as shown in Fig.…”

Clinical significance and potential mechanisms of miR-223-3p and miR-204-5p in squamous cell carcinoma of head and neck: a study based on TCGA and GEO

“…This result needs a thorough study to explore the definite role of miR-142-3p in SCCHN. Certain miRNAs are helpful for cancer diagnoses [ 38 ]. In view of this, the present study distinguished five miRNAs with possible diagnostic values, which consisted of miR-503-5p , miR-424-5p , miR-21-5p , miR-204-5p , and miR-375-3p .…”

HPV-Related Oropharyngeal Cancer and Biomarkers Based on Epigenetics and Microbiome Profile

“…Investigation of individual miRNA molecules may be of use for the benefit of diagnostic and prognostic value in HNSCC, but when it comes to a combination of miRNAs, provides a more significant prediction in clinical outcomes. The diagnostic power of miR-383, miR-615, and miR-877 panel distinguishes patients with HNSCC from healthy donors, with high rates of a receiver operating characteristic (ROC) curve analysis (89.3% sensitivity and 98.9% specificity) ( Liu et al, 2019 ). Given the unfavorable prognosis of HPV-negative cancer patients strengthens the need to find biomarkers through miRNA signatures for a more accurate diagnosis ( Hess et al, 2019 ).…”