Combined In Vitro/In Vivo Test Procedure with Human Tumor Xenografts for New Drug Development

Hh, Fiebig; Winterhalter, B. R.; Dp, Berger; Gw, Löhr

doi:10.1159/000421298

Cited by 55 publications

(40 citation statements)

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“…Cell lines derived from the Oncotest collection were established from human tumor xenografts as described by Roth et al (1999). The origin of the donor xenografts was described by Fiebig et al (1992Fiebig et al ( , 1999. The other cell lines were kindly provided by the National Cancer Institute (Bethesda, MD, USA).…”

Section: Antitumor Activitymentioning

confidence: 99%

Characterization of the anticancer properties of monoglycosidic cardenolides isolated from Nerium oleander and Streptocaulon tomentosum

Rashan

Franke

Khine

et al. 2011

Journal of Ethnopharmacology

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Section: Antitumor Activitymentioning

confidence: 99%

Characterization of the anticancer properties of monoglycosidic cardenolides isolated from Nerium oleander and Streptocaulon tomentosum

Rashan

Franke

Khine

et al. 2011

Journal of Ethnopharmacology

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“…1 Cell Culture. The human tumor xenograft cell lines LXFL529L (large cell lung carcinoma), GXF251L (gastric carcinoma) and CXF97 (colorectal carcinoma) (13) were cultured in RPMI 1640 medium, all with 10% fetal calf serum (FCS) and 1% penicillin/streptomycin in humidified incubators (37°C, 5% CO2). Cell culture medium and supplements were obtained from Gibco Life Technologies (Karlsruhe, Germany).…”

Section: ) 2-hydroxy-2(2′3′-dihydroxy-5′-methyl-2′-cyclopentenon-5′mentioning

confidence: 99%

Maillard Reaction Products Modulating the Growth of Human Tumor Cells in Vitro

Marko

Habermeyer

Kemény

et al. 2002

Chem. Res. Toxicol.

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We investigated the effect of a series of Maillard reaction products formed from carbohydrates under household heating conditions on the growth of human tumor cells in vitro. 4-Hydroxy-5-methyl-3-(2H)-furanone (1) was found to potently enhance the proliferation of human tumor cells. In contrast, the Maillard-type chromophores 2-(2-furyl)methylidene-4-hydroxy-5-methyl-2H-furan-3-one (2), 4-(2-furyl)-7-[(2-furyl)methylidene]-2-hydroxy-2H,7H,8aH-pyrano[2,3-b]- pyran-3-one (6), and 3-hydroxy-4[(E)-(2-furyl)methylidene]methyl-3-cyclopentene-1,2 dione (13) inhibited the growth of human tumor cells in vitro in the low micromolar range. GXF251L cells (gastric carcinoma), synchronized by serum deprivation, were retained in the G1-phase of the cell cycle after treatment with 2, 6, or 13 for 24 h. Concomitantly, a distinct sub-G1 peak was observed, indicative for apoptosis induction. DNA fragmentation was further investigated by ELISA using antibodies raised against histones and DNA. 2 induced a significant increase of fragmented DNA at concentrations > or = 30 microM. After treatment with compound 6, DNA fragmentation was observed at a higher concentration range (> or = 50 microM), whereas incubation with 13 resulted in a marked DNA fragmentation already at 20 microM. On the protein level, the activation of caspase 3, as an early marker for apoptosis induction, was determined. The results were almost identical to those obtained in the DNA fragmentation ELISA. In summary, Maillard reaction products potently modulating the growth of human tumor cells were identified. The Maillard-type chromophores 2, 6, and 13 were found to interfere with the proliferation of gastric carcinoma cells, causing cell cycle arrest and apoptosis induction.

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“…Twenty-four out of the 36 cell lines were established from patient tumors passaged as subcutaneous xenografts in nude mice. [7][8][9] To get clues as to their mechanisms of action, following analysis in the 36 cell line panel of the monolayer assay, novel compounds can be subjected to Compare Analysis. For this, the compounds' IC 70 activity profiles in the 36 cell line panel are compared to the available corresponding activity profiles determined in the same cell lines for more than 100 reference compounds representing diverse mechanisms of action.…”

mentioning

confidence: 99%