Combined inoculation of rhizobia and Trichoderma spp. on cowpea in the savanna, Gurupi-TO, Brazil

Abstract

The goal of this study was to evaluate the combined inoculation of rhizobia and Trichoderma spp. in cowpea, their ability to promote growth and use as a biological control agent for foliar blight (Rhizoctonia solani). The study was conducted in a field experiment in a randomized block design with four replications. The treatments consisted of seed-applied rhizobia with and without Trichoderma spp., and to the soil at planting time and 15 days after planting (DAP). The rhizobia inoculation was performed with a mixture of strains INPA 03-11B and UFLA 03-84. For the treatments with Trichoderma spp., a commercial powder product, Trichoplus JCO was used. The application of Trichoderma spp. and rhizobia at 15 DAP, in both the seed and in the soil, showed the best results (p < 0.05). The application of Trichoderma spp. did not inhibit the nodulation process, and the best results were obtained when the fungus was inoculated with rhizobia. The inoculation treatments were positive for Trichoderma spp. The Trichoderma spp. treatments showed improvements in stand counts, survival and effectiveness against R. solani, reinforcing that the usage of Trichoderma spp. as a seed and soil treatment.

Plant Growth Stimulation by Microbial Consortia

“…and Trichoderma spp. had positive effects on total shoot and root dry weight of cowpea plants, and it protected the plants against Rhizoctonia solani infection, especially when the initial co-inoculation was followed by a re-inoculation of Trichoderma 15 days later [177].…”

Plant growth-promoting bacteria potentiate antifungal and plant-beneficial responses of Trichoderma atroviride by upregulating its effector functions

“…For example, co-inoculation of Trichoderma spp. and rhizobia increased the biomass of V. unguinculata when inoculated in soil or by coating the seeds [71]. In addition, inoculation with T. atroviride MUCL45632 and the mycorrhizal fungus Glomus intraradices greatly improved shoot and root dry weights of tomato, zucchini, and lettuce plants, with better results than inoculation with the single organisms [73].…”

“…The combined inoculation of Trichoderma spp. and rhizobia in soil and Vigna unguiculata seeds resulted in less disease severity and symptoms caused by R. solani [71], showing greater biocontrol ability over the pathogen. A synthetic microbial community (SynCom) formed with several isolates of Pseudomonas spp., Bacillus spp., Streptomyces spp., and Trichoderma spp.…”

Trichoderma como promotor de crescimento de mudas de eucaliptos

“…Os resultados positivos observados para os diferentes experimentos podem ser explicados em função da ação do inoculante utilizado, tendo em vista que fungos do gênero Trichoderma são utilizadas não só no controle biológico de fitopatógenos, mas como promotores de crescimento vegetal, devido a sua versatilidade de ação, como parasitismo, antibiose e competição, além de atuarem como indutores de resistência a plantas contra doenças e produzirem hormônios de crescimento, solubilização de fosfato, sideróforos e metabólitos secundários (Milanesi et al, 2013;Chagas Junior et al, 2015;Contreras-Cornejo et al, 2016;Bononi et al, 2020). Estes fungos encontram-se na rizosfera, são promotores do crescimento em espécies vegetais, e produzem uma rica fonte de metabólitos secundários, apresentando um vasto repertório de genes supostamente envolvidos na biossíntese de peptídeos não ribossômicos, policetideos, terpenóides e pironas (Mukherjee et al, 2012), e a inoculação com uma concentração alta desse microrganismos pode proporcionar resultados positivos quanto ao biocontrole de fitopatógenos e, consequentemente, a promoção do crescimento vegetal.…”