Combined interactions of amino acids and organic acids in heavy metal binding in plants

Kocaman, Ayhan

doi:10.1080/15592324.2022.2064072

Cited by 38 publications

(19 citation statements)

References 73 publications

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“…Heavy metals can form complexes with organic acids, resulting in changes in their toxicity and migration ability [43]. Previous studies have shown that the formation of strong bonds between chelating metals and carboxylate groups and phenolic hydroxide groups of organic acids plays an important role in metal accumulation and detoxification in plants [44,45]. Among organic acids, oxalate is a strong dicarboxylic acid anion and a good complexing agent for the binding of heavy metal cations [46,47].…”

Section: Resultsmentioning

confidence: 99%

Effects of Free Amino Acids, Metallothionein, and Chemical Forms of Heavy Metals on the Accumulation and Detoxification of Cadmium and Chromium in Chinese Goldthread (Coptis chinensis Franch.)

Huang

Niu

et al. 2022

Journal of Sensors

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Chinese goldthread (Coptis chinensis Franch.) is an important medicinal plant worldwide. However, its clinical safety is negatively impacted by high cadmium (Cd) and chromium (Cr) levels. Understanding the accumulation pattern and detoxification mechanism of heavy metals, such as Cd and Cr, is important for regulating their transportation and reducing their accumulation in plants. Free amino acids (FAAs), metallothioneins, and chemical forms of heavy metals are closely associated with heavy metal accumulation and detoxification mechanisms. In the present study, we evaluated the uptake of Cd and Cr in Chinese goldthread and characterized the changes in FAA levels, CcMT2, a metallothionein gene, expression under Cd and Cr stress, and Cr chemical forms in various tissues of Chinese goldthread. The majority of Cd and Cr was retained in the roots; this might be the tolerance strategy utilized by Chinese goldthread to protect the aboveground photosynthetic tissues. A chemical continuous extraction method was utilized to analyze the chemical forms of Cr, and the results suggested that Cr was primarily fixed by oxalate in all tissues, and after Cr treatment, Cr tended to combine with phosphate to form an insoluble complex that reduced the toxicity and bioavailability of Cr in Chinese goldthread. The accumulation of Cd and Cr was significantly positively correlated with valine, histidine, threonine, tyrosine, and serine levels and significantly negatively correlated with glutamic acid and alanine levels. The variation in proline levels under Cd stress was different than that under Cr stress. FAAs might affect the accumulation of and resistance to Cd and Cr through various detoxification strategies, such as chelation with heavy metals ions and conversion into the required products. Furthermore, CcMT2 was isolated for the first time and found to be dominantly expressed in leaves. CcMT2 was induced under Cd and Cr stress in all plant tissues, indicating its role in the accumulation of and tolerance to Cd and Cr.

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Section: Resultsmentioning

confidence: 99%

Effects of Free Amino Acids, Metallothionein, and Chemical Forms of Heavy Metals on the Accumulation and Detoxification of Cadmium and Chromium in Chinese Goldthread (Coptis chinensis Franch.)

Huang

Niu

et al. 2022

Journal of Sensors

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“…In this study, the number of differential metabolites of Trichoderma Ts1 increased with the increase in the nuclide concentration, and the differential metabolites of organic acids and derivatives and of organic oxygen compounds increased signi cantly. Studies have shown that organic acids can reduce the available state of soil heavy metals and chelate heavy metals in organisms (Kocaman 2022;Zhao et al 2022). The data from this study indicated that the amino acid and carbohydrate metabolic pathways of Trichoderma Ts1 were altered.…”

Section: Discussionmentioning

confidence: 63%

Bioconcentration and Tolerance Mechanism of a Trichoderma Strain Exposed to Cobalt, Strontium, and Uranium

Li¹,

Lai²,

Li³

et al. 2022

Preprint

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Microbial enrichment of radionuclides has the advantages of low cost, simple operation, and no secondary pollution, but the enrichment abilities of radionuclides differ. In this study, a Trichoderma fungus with high tolerance to a variety of radionuclides [i.e., cobalt (Co), strontium (Sr), and uranium (U)] was screened from radioactive contaminated soil by analyzing the enrichment characteristics. Combined with non-targeted metabolomics technology, the physiological response mechanism of Trichoderma fungus metabolism to cobalt, strontium and uranium exposure was revealed from the metabolic level. The results showed that the Trichoderma grew well under multi-nuclides exposure, and the Co, Sr, and U accumulated on the mycelium surface of the strain. Their enrichment reached 36.4–96.6 mg/kg, 76.7–239.7 mg/kg, and 268.5–667.0 mg/kg, respectively. With the increase in the multi-nuclides exposure concentration, the element metabolism of the strain changed, and U, Co, and Sr showed a cooperative absorption relationship. The gas chromatograph-mass spectrometer (GC-MS) non-targeted metabolome analysis showed that the amino acid metabolic pathway and the carbohydrate pathway of the strain changed when the Co, Sr, and U concentrations were 100 mg/L. The results showed that Trichoderma has strong enrichment potential and tolerance to Co, Sr, and U and can be used for radionuclide removal.

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“…The accumulation of organic and amino acids can chelate heavy metal ions and scavenge reactive oxygen species (ROS), thereby reducing intracellular oxidative damage [21]. Heavy metals can be chelated with low molecular weight organic acids stored in vacuoles and transformed into low or non-toxic chelating states, signi cantly contributing to plant heavy metal stress resistance [47,48]. For example, citric acid can alleviate Cd stress by reducing plants' Cd uptake [24].…”

Section: Role Of Organic Acids Under CD Stressmentioning

confidence: 99%

“…Plants adapt to abiotic stress by regulating self-metabolism; consequently, amino acids, fatty acids, and organic acids have been extensively studied [19][20][21]. Plants regulate the metabolic process of amino acids, organic acids, and fatty acids to cope with heavy metal toxicity; these metabolites vary with the plant variety or genotype [22,23].…”

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confidence: 99%

Physiological and metabolomic analyses provide insights into the cadmium tolerance of Sophora tonkinensis

Chen

Huang

Tang

et al. 2023

Preprint

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Sophora tonkinensis is an important medicinal plant native to China and Vietnam, growing in stony mountains and calcareous or sandstone hill slopes. Cadmium negatively affects the quality of S. tonkinensis, indirectly threatening human health. To elucidate the physiological and metabolic mechanism of S. tonkinensis in response to Cd stress, we conducted a hydroponic greenhouse experiment and measured the growth parameters of S. tonkinensis treated with 0, 20, 40, 60, and 80 µmol L− 1 Cd. Furthermore, metabolite changes in S. tonkinensis roots were investigated using liquid chromatography–tandem mass spectrometry. We identified 380 differential metabolites, and significant differences were detected in the varieties and quantities of amino acids, organic acids, fatty acids, and ketones, which have significant impact on S. tonkinensis stress response to Cd, under different Cd concentrations. S. tonkinensis resisted the stress induced by 40 µmol L− 1 Cd by upregulating lysine, arginine, histidine, glycine, sarcosine, tryptophan, D-proline, DL-serine, cellobiose, linoleic, indole, cytosine, and genistein contents and downregulating betaine, leucine, citrate, oleic, trehalose, and daidzein contents. Linoleic, palmitic, citrate, maackiain, isomaltose, and cinnavalininate contents were upregulated, and L-phenylalanine, sarcosine, indole acetate, and cytosine were downregulated in response to 80 µmol L− 1 Cd stress. KEGG pathway enrichment analysis showed that the isoflavonoid biosynthesis pathway was significantly enriched, indicating its important role in the Cd resistance of S. tonkinensis. This study provides important information for understanding the tolerance and accumulation mechanisms of S. tonkinensis in response to Cd stress, which will contribute to the breeding of medicinal plant.

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Combined interactions of amino acids and organic acids in heavy metal binding in plants

Cited by 38 publications

References 73 publications

Effects of Free Amino Acids, Metallothionein, and Chemical Forms of Heavy Metals on the Accumulation and Detoxification of Cadmium and Chromium in Chinese Goldthread (Coptis chinensis Franch.)

Effects of Free Amino Acids, Metallothionein, and Chemical Forms of Heavy Metals on the Accumulation and Detoxification of Cadmium and Chromium in Chinese Goldthread (Coptis chinensis Franch.)

Bioconcentration and Tolerance Mechanism of a Trichoderma Strain Exposed to Cobalt, Strontium, and Uranium

Physiological and metabolomic analyses provide insights into the cadmium tolerance of Sophora tonkinensis

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