Combined Multilocus Sequence Typing and O Serogrouping Distinguishes<i>Escherichia coli</i>Subtypes Associated with Infant Urosepsis and/or Meningitis

Bidet, Philippe; Mahjoub-Messai, Farah; Blanco, Jorge; Blanco, Jesús E.; Dehem, Marie; Aujard, Y.; Bingen, Édouard; Bonacorsi, Stéphane

doi:10.1086/518897

Cited by 92 publications

(84 citation statements)

References 33 publications

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“…The E. coli strains were assigned to one of the four main phylogenetic groups (A, B1, B2, and D) by using a previously described multiplex PCR method (15) that uses a combination of 3 DNA markers (chuA, yjaA, and TSPE4.C2). Using a PCR-based method, the strains were also screened for 11 genes encoding the following putative virulence factors (VFs): fyuA, iron uptake; hly, hemolysin; sfa/foc, S or F1C fimbriae; papC genes of P fimbriae operons; iucC, aerobactin; papG (II and III alleles); cnf1, cytotoxic necrotizing factor; iroN, salmochelin receptor; ibeA, putative invasin; hra, heatresistant agglutinin; and the capsular antigen K1 (16,17).…”

Section: Settingmentioning

confidence: 99%

Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Strains Involved in Maternal-Fetal Colonization: Prevalence of E. coli ST131

et al. 2013

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dMaternal-fetal Escherichia coli infections, such as neonatal bacteremia and meningitis, are important causes of morbidity and mortality. From 2006 to 2010, we studied newborns and their mothers who were colonized with E. coli in a French hospital in order to document (i) the epidemiology and genetic characteristics of extended-spectrum-beta-lactamase (ESBL)-producing E. coli strains, (ii) the prevalence of associated virulence genes, (iii) the prevalence of clone sequence type 131 (ST131), and (iv) the genetic relationship among ESBL-producing strains. Among the 2,755 E. coli cultures recovered from vaginal or neonatal samples, 68 were ESBL producers (2.46%). We found a wide diversity of ESBL genes, with the majority being bla CTX-M-14 , bla CTX-M-1 , and bla CTX-M-15 , distributed among the 4 main phylogenetic groups. Genes encoding virulence factors were found in 90.7% of the isolates, with >2 virulence genes present in 76% of cases. The prevalence of ST131 among ESBL-producing E. coli isolates was 9.4% (6/64). Five of these 6 ST131 isolates possessed bla CTX-M-15 enzymes (and also were resistant to quinolones), and one possessed bla CTX-M-2 enzymes. Two possessed virulence genes, suggesting the presence of pathogenicity island II J96 (PAI II J96 )-like domains. Pulsed-field gel electrophoresis (PFGE) revealed a high level of genomic diversity overall, except for 3 closely related isolates belonging to clonal group ST131. Repetitive PCR showed that the six ST131 isolates were closely related to ST131 control strains (>95% similarity). This study shows a high prevalence of ESBL-producing E. coli strains and clonal group ST131 in the French maternal-fetal population. These results suggest a widespread distribution of ESBL enzymes in the community and highlight the early transmission between mothers and neonates. These findings are worrisome, especially for this particularly vulnerable population. In the past decade, there has been an alarming increase in antibiotic-resistant Enterobacteriaceae producing extended-spectrum beta-lactamases (ESBLs), due at least in part to the overuse of broad-spectrum cephalosporins.The majority of ESBLs identified in clinical isolates during the 1980s to 1990 were of the SHV or TEM type (1). However, since the mid-2000s, CTX-M ESBLs, originating from environmental Kluyvera spp., have gained prominence and are considered pandemic enzymes. CTX-M has been identified in several members of the Enterobacteriaceae family, and especially in Escherichia coli, which has replaced Klebsiella spp. as the principal ESBL-producing member of the Enterobacteriaceae (2-4).ESBL enzymes are important causes of cephalosporin treatment failure. E. coli strains carrying these enzymes were initially responsible mainly for nosocomial infections, but they have now disseminated into the community. One of the best examples of this trend is the global spread of E. coli clone sequence type 131 (ST131), expressing CTX-M enzymes (5). Maternal-fetal E. coli infections, such as neonatal bacteremia and meningitis,...

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Section: Settingmentioning

confidence: 99%

Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Strains Involved in Maternal-Fetal Colonization: Prevalence of E. coli ST131

et al. 2013

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“…Overall, carriage of a uropathogenic clone was more common (median 4 weeks) than carriage of a commensal clone (median 1 week). For example, ST29, a clone associated with infant urosepsis and/ or meningitis 12 was the most common clone isolated in children with neurogenic bladder. Child no.…”

Section: Carriage Of Clonesmentioning

confidence: 99%

Escherichia coli colonizing the neurogenic bladder are similar to widespread clones causing disease in patients with normal bladder function

et al. 2008

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Study design: Clonal typing of neurogenic clones. Objective: To determine whether neurogenic clones carried over weeks in the urine of asymptomatic children with neurogenic bladder were similar to known uropathogenic clones associated with disease. Setting: Michigan State University; VA Medical Center, Minneapolis, MN, USA. Methods: Escherichia coli isolates from the urine of 15 children previously followed were typed by multilocus sequence typing and compared to 2 human pyelonephritis genome strains, 29 pediatric or adult symptomatic urinary tract infection strains, 15 pediatric asymptomatic bacteriuria strains, 6 animal urinary tract infection strains and a neonatal meningitis-septicemia prototype K1 strain. Phylotypes and virulence genotypes were determined using PCR. Results: Twenty-nine E. coli isolates were classified into 15 clones. Six of 15 clones were the same sequence type or a close relative to a clone that caused disease in a human or animal. These clones were considered uropathogens. The remaining nine clones were not closely related to a clone that caused disease and were considered commensal clones. Uropathogens were predominantly group B2, exhibited more virulence genes and were carried for more weeks in the neurogenic bladder compared to commensal clones. Nine of 15 children carried one or more uropathogenic clones; 4 children carried one or more commensal clones and 2 children carried both uropathogenic and commensal clones. Conclusion: Children with neurogenic bladder most commonly carried commensal clones. Uropathogenic clones were associated with prolonged carriage, however, carriage was not associated with symptomatic disease or deterioration of the upper urinary tract.

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“…For the discriminatory power to be stronger from the epidemiological point of view, it will be necessary to use it in combination with other typing methods such as plasmid profiles, virulence gene typing, antimicrobial resistance testing, multi-locus sequence typing, fimH single-nucleotide polymorphism typing, and phylogenetic grouping [14][15][16][17][18]. When there is change in the subtype of the PAIusp in the locality, this will trigger the attention of the public health officer to trace the source of infections of this new subtype.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of PAIusp subtyping to characterize uropathogenic E. coli isolates

Lai

Zaw

Shamsudin

et al. 2016

J Infect Dev Ctries

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Introduction: Uropathogenic virulence factors have been identified by comparing the prevalence of these among urinary tract isolates and environmental strains. The uropathogenic-specific protein (USP) gene is present on the pathogenicity island (PAI) of uropathogenic Escherichia coli (UPEC) and, depending on its two diverse gene types and the sequential patterns of three open reading frame units (orfUs) following it, there is a method to characterize UPEC epidemiologically called PAIusp subtyping. Methodology: A total of 162 UPEC isolates from Sabah, Malaysia, were tested for the presence of the usp gene and the sequential patterns of three orfUs following it using polymerase chain reaction (PCR). In addition, by means of triplex PCR, the prevalence of the usp gene was compared with other two VFs of UPEC, namely alpha hemolysin (α-hly) and cytotoxic necrotizing factor (cnf-1) genes encoding two toxins. Results: The results showed that the usp gene was found in 78.40% of UPEC isolates, indicating that its prevalence was comparable to that found in a previous study in Japan. The two or three orfUs were also associated with the usp gene in this study. All the PAIusp subtypes observed in Japan were present in this study, while subtype IIa was the most common in both studies. The usp gene was observed in a higher percentage of isolates when compared with ɑ-hly and cnf-1 genes. Conclusions: The findings in Japan and Sabah, East Malaysia, were similar, indicating that PAIusp subtyping is applicable to the characterization of UPEC strains epidemiologically elsewhere in the world.

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Combined Multilocus Sequence Typing and O Serogrouping DistinguishesEscherichia coliSubtypes Associated with Infant Urosepsis and/or Meningitis

Cited by 92 publications

References 33 publications

Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Strains Involved in Maternal-Fetal Colonization: Prevalence of E. coli ST131

Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Strains Involved in Maternal-Fetal Colonization: Prevalence of E. coli ST131

Escherichia coli colonizing the neurogenic bladder are similar to widespread clones causing disease in patients with normal bladder function

Evaluation of PAIusp subtyping to characterize uropathogenic E. coli isolates

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