Combined multiplex loop-mediated isothermal amplification with lateral flow assay to detect sea
 and seb
 genes of enterotoxic Staphylococcus aureus

Yin, Hsin‐Yi; Fang, Tony J.; Wen, Hsiao‐Wei

doi:10.1111/lam.12590

Cited by 34 publications

(21 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although, many previous studies have applied LFB for the analysis of LAMP amplicons, the basic mechanism did not be depicted (Kiatpathomchai et al, 2008 ; Njiru, 2012 ; Kaewphinit et al, 2013 ; Yin et al, 2016 ). To the best of our knowledge, this is first report expounding the basic LAMP-LFB principle and providing the details on biosensor detection (Figures 1 , 2 ).…”

Section: Discussionmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification Label-Based Gold Nanoparticles Lateral Flow Biosensor for Detection of Enterococcus faecalis and Staphylococcus aureus

Wang

et al. 2017

Front. Microbiol.

View full text Add to dashboard Cite

The report describes a simple, rapid and sensitive assay for visual and multiplex detection of Enterococcus faecalis and Staphylococcus aureus based on multiple loop-mediated isothermal amplification (mLAMP) and lateral flow biosensor (LFB). Detection and differentiation of the Ef0027 gene (E. faecalis-specific gene) and nuc gene (S. aureus-specific gene) were determined using fluorescein (FITC)-and digoxin-modified primers in the mLAMP process. In the presence of biotin- and FITC-/digoxin-modified primers, the mLAMP yielded numerous biotin- and FITC-/digoxin-attached duplex products, which were detected by LFB through biotin/streptavidin interaction (biotin on the duplex and streptavidin on the gold nanoparticle) and immunoreactions (FITC/digoxin on the duplex and anti-FITC/digoxin on the LFB test line). The accumulation of gold nanoparticles generated a characteristic red line, enabling visual and multiplex detection of target pathogens without instrumentation. The limit of detection (LoD), analytical specificity and feasibility of LAMP-LFB technique were successfully examined in pure culture and blood samples. The entire procedure, including specimen (blood samples) processing (30 min), isothermal reaction (40 min) and result reporting (within 2 min), could be completed within 75 min. Thus, this assay offers a simple, rapid, sensitive and specific test for multiplex detection of E. faecalis and S. aureus strains. Furthermore, the LAMP-LFB strategy is a universal technique, which can be extended to detect various target sequences by re-designing the specific LAMP primers.

show abstract

Section: Discussionmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification Label-Based Gold Nanoparticles Lateral Flow Biosensor for Detection of Enterococcus faecalis and Staphylococcus aureus

Wang

et al. 2017

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Despite these issues, the DNA strip approach developed in this study has several advantages, including its rapidity, multiplicity, and no need for special equipment, over other detection methods, such as conventional PCR, immunochromatography, and real-time PCR. Of further note, LAMP-lateral flow combined assays have also been recently developed for the detection of several other pathogenic microbes, such as Toxoplasma [17] and Staphylococcus aureus [25, 27].…”

mentioning

confidence: 99%

Detection of Sarcocystis spp. and Shiga toxin-producing Escherichia coli in Japanese sika deer meat using a loop-mediated isothermal amplification-lateral flow strip

Sugita-Konishi

Kobayashi

Takasaki³

et al. 2019

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

Game meat potentially harbors a number of parasitic and bacterial pathogens that cause foodborne disease. It is thus important to monitor the prevalence of such pathogens in game meats before retail and consumption to ensure consumer safety. In particular, Sarcocystis spp. and Shiga toxin-producing Escherichia coli (STEC) have been reported to be causative agents of food poisoning associated with deer meat consumption. To examine the prevalence of these microbiological agents on-site at a slaughterhouse, the rapid, simple and sensitive detection method known as the “DNA strip” has been developed, a novel tool combining loop-mediated isothermal amplification and a lateral flow strip. This assay has achieved higher sensitivity and faster than conventional PCR and is suitable for on-site inspection.

show abstract

“…Depending on the subsequent detection method, this amplification scheme may be modified to incorporate a labeled probe. 84,85 This amplification scheme is highly efficient, capable of amplifying a few copies of genetic material to 10 9 copies within 1 h. Furthermore, additional primers increase the specificity of this technique. Among isothermal amplification techniques, 78% of recent publications for food-based analysis are LAMP-based.…”

Section: Detection Of Nucleic Acidsmentioning

confidence: 99%

Point-of-Need Diagnostics for Foodborne Pathogen Screening

et al. 2021

View full text Add to dashboard Cite

Combined multiplex loop-mediated isothermal amplification with lateral flow assay to detect sea and seb genes of enterotoxic Staphylococcus aureus

Cited by 34 publications

References 28 publications

Loop-Mediated Isothermal Amplification Label-Based Gold Nanoparticles Lateral Flow Biosensor for Detection of Enterococcus faecalis and Staphylococcus aureus

Loop-Mediated Isothermal Amplification Label-Based Gold Nanoparticles Lateral Flow Biosensor for Detection of Enterococcus faecalis and Staphylococcus aureus

Detection of <i>Sarcocystis</i> spp. and Shiga toxin-producing <i>Escherichia coli</i> in Japanese sika deer meat using a loop-mediated isothermal amplification-lateral flow strip

Point-of-Need Diagnostics for Foodborne Pathogen Screening

Contact Info

Product

Resources

About