Combined phospho‐ and glycoproteome enrichment in nephrocalcinosis tissues of phytate‐fed rats

Tran, TrangHuyen; Park, Jong; Kim, Ok-Hee; Kim, Bora; Choi, Dong‐Hee; Lee, JeongHwa; Kim, KwangPyo; Oh, Byung‐Chul; Lee, Hookeun

doi:10.1002/rcm.6742

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…Capture of phosphopeptides on TiO 2 particles, and of N‐ linked glycopeptides on hydrazide beads, resulted in the identification of 358–369 phosphosites and of 437–468 glycosites in the normal and diseased tissues. An LTQ‐XL was used for phosphopeptide and a QTOF for deglycosylated peptide detection, respectively . The integrated nature of the study also revealed the broader implications of these PTMs on the pathological status of cells.…”

Section: Technological Developments For the Analysis Of N/o‐glycoprotmentioning

confidence: 99%

Exploring the glycoproteomics landscape with advanced MS technologies

et al. 2014

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The advance of glycoproteomic technologies has offered unique insights into the importance of glycosylation in determining the functional roles of a protein within a cell. Biologically active glycoproteins include the categories of enzymes, hormones, proteins involved in cell proliferation, cell membrane proteins involved in cell-cell recognition, and communication events or secreted proteins, just to name a few. The recent progress in analytical instrumentation, methodologies, and computational approaches has enabled a detailed exploration of glycan structure, connectivity, and heterogeneity, underscoring the staggering complexity of the glycome repertoire in a cell. A variety of approaches involving the use of spectroscopy, MS, separation, microfluidic, and microarray technologies have been used alone or in combination to tackle the glycoproteome challenge, the research results of these efforts being captured in an overwhelming number of annual publications. This work is aimed at reviewing the major developments and accomplishments in the field of glycoproteomics, with focus on the most recent advancements (2012-2014) that involve the use of capillary separations and MS detection.

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Section: Technological Developments For the Analysis Of N/o‐glycoprotmentioning

confidence: 99%

Exploring the glycoproteomics landscape with advanced MS technologies

et al. 2014

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Metal oxides in sample pretreatment

et al. 2016

TrAC Trends in Analytical Chemistry

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Decoding Kidney Pathophysiology: Omics-Driven Approaches in Precision Medicine

Delrue,

Speeckaert

2024

JPM

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Chronic kidney disease (CKD) is a major worldwide health concern because of its progressive nature and complex biology. Traditional diagnostic and therapeutic approaches usually fail to account for disease heterogeneity, resulting in low efficacy. Precision medicine offers a novel approach to studying kidney disease by combining omics technologies such as genomics, transcriptomics, proteomics, metabolomics, and epigenomics. By identifying discrete disease subtypes, molecular biomarkers, and therapeutic targets, these technologies pave the way for personalized treatment approaches. Multi-omics integration has enhanced our understanding of CKD by revealing intricate molecular linkages and pathways that contribute to treatment resistance and disease progression. While pharmacogenomics offers insights into expected responses to personalized treatments, single-cell and spatial transcriptomics can be utilized to investigate biological heterogeneity. Despite significant development, challenges persist, including data integration concerns, high costs, and ethical quandaries. Standardized data protocols, collaborative data-sharing frameworks, and advanced computational tools such as machine learning and causal inference models are required to address these challenges. With the advancement of omics technology, nephrology may benefit from improved diagnostic accuracy, risk assessment, and personalized care. By overcoming these barriers, precision medicine has the potential to develop novel techniques for improving patient outcomes in kidney disease treatment.

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Combined phospho‐ and glycoproteome enrichment in nephrocalcinosis tissues of phytate‐fed rats

Cited by 3 publications

References 36 publications

Exploring the glycoproteomics landscape with advanced MS technologies

Exploring the glycoproteomics landscape with advanced MS technologies

Metal oxides in sample pretreatment

Decoding Kidney Pathophysiology: Omics-Driven Approaches in Precision Medicine

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