Combined Q-banding and fluorescence in situ hybridization for the identification of bovine chromosomes 1 to 7

Abstract

Eleven probes were assigned to bovine chromosomes 1 to 7 by fluorescence in situ hybridization (FISH). The identification of chromosomes was based on QFQ-banding prior to in situ hybridization and comparison with the Reading Conference (1976) and ISCNDA (1989) standards. The probes used for FISH can now be utilized as identification and discrimination features for bovine chromosomes 1 to 7 and particularly for chromosomes 4 and 6, which are difficult to distinguish. Comparison of our mapping data with previous assignments and of the standard chromosome banding patterns prompt us to propose a change in the ISCNDA nomenclature: ISCNDA chromosome 4 should be named chromosome 6 and vice versa. Chromosome 4 is marked by the ribosomal RNA cluster RNR3, and chromosome 6 is characterized by the casein gene cluster and an anonymous satellite (D6Z1).

Physical assignment of microsatellite- containing BACs to bovine chromosomes

“…Our assignments, as well as the previous assignments of microsatellite markers, have shown almost complete chromosome coverage for BTA 2, BTA 10, and BTA 28 (Solinas-Toldo et al, 1995;Heaton et al, 1997;Smith et al, 1997;Sonstegard et al, 1997). The estimated physical/genetic relationships for these chromosomes are 1.28, 1.09, and 0.99 Mb/cM, respectively (154, 110, and 52 Mb estimated physical sizes and 120, 101, and 52 cM genetic length, respectively).…”

Characterization and chromosome localization of a processed pseudogene related to the bovine laminin receptor gene family

“…FISH was performed as described previously by Toldo et al (1993) and Solinas-Toldo et al (1995) using bovine metaphase spreads (prepared from peripheral lymphocytes) obtained from a normal, healthy bull. Prior to FISH, the QFQ-banded spreads were photographed using a cooled CCD camera.…”

Centromere and telomere redistribution precedes homologue pairing and terminal synapsis initiation during prophase I of cattle spermatogenesis

“…The application of this probe, however, required suppression hybridisation of repetitive sequences, which precluded its combinatorial use with pericentromeric or telomeric DNA probes. The same restriction applied to probe p33E39 specific for the subcentromeric region of BTA6 (Solinas-Toldo et al, 1995). Plasmid BR1.3 (pBR1.3; Kurnit et al, 1973) was used to probe the heterochromatin of the pericentromeric region of all bovine autosomes.…”