Combined serum IgG response to<i>Helicobacter pylori</i>VacA and CagA predicts gastric cancer

Janulaitytė-Gunther, Daiva; Kupčinskas, Limas; Pavilonis, Alvydas; Valuckas, Konstantinas Povilas; Wadström, Torkel; Andersen, Leif Percival

doi:10.1111/j.1574-695x.2007.00268.x

Cited by 15 publications

(13 citation statements)

References 23 publications

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“…Some studies have even found stronger associations for gastrointestinal disease among individuals with VacA antibodies than among those with CagA antibodies (32, 33, 34). However, overall, the findings for the association of VacA antibodies and gastrointestinal pathology are inconsistent, with studies that find positive associations with disease (32, 34, 35) and those that find null associations (31, 36). …”

Section: Discussionmentioning

confidence: 99%

“…In regards to gastric cancer, serum antibodies to both CagA and VacA have been found to be associated with increased risk of disease as well as a stronger inflammatory response (31). Some studies have even found stronger associations for gastrointestinal disease among individuals with VacA antibodies than among those with CagA antibodies (32, 33, 34).…”

Section: Discussionmentioning

confidence: 99%

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Helicobacter pylori Protein–Specific Antibodies and Risk of Colorectal Cancer

Epplein

Pawlita

Michel

et al. 2013

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Background There is biological plausibility as to why infection with Helicobacter pylori, the leading cause of gastric cancer, may also increase the risk of colorectal cancer, but the epidemiological findings have been inconsistent. We assessed the association of H. pylori protein-specific infection and colorectal cancer risk in the prospective cohort, the Southern Community Cohort Study. Methods Multiplex serology was utilized to measure antibodies to 15 H. pylori proteins in pre-diagnostic blood among 188 incident colorectal cancer cases and 370 controls matched by age, race, sex, and blood collection timing. Conditional logistic regression was used to calculate ORs and 95% confidence intervals (CI). Results Overall H. pylori prevalence was not associated with colorectal cancer risk (OR, 1.03; 95% CI, 0.59–1.77). However, sero-positivity to any of five specific H. pylori proteins (VacA, HP231, HP305, NapA, HcpC) was associated with a significant 60 to 80% increase in odds of risk. These associations became even stronger when limited to colon cancer risk, particularly for the known H. pylori toxin VacA (OR, 2.24; 95% CI, 1.22–4.11), including a significant, positive dose-response association by VacA antibody levels in quartiles (P<0.05). Associations with VacA sero-positivity were especially strong for early onset and late stage cancers. Conclusions The findings raise the hypothesis that individuals with high levels of antibodies to specific H. pylori proteins may be at higher risk of colon cancer. Impact Further investigation of the H. pylori – colorectal cancer association is warranted to determine the possibility of protein-specific antibody levels as a risk biomarker.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Helicobacter pylori Protein–Specific Antibodies and Risk of Colorectal Cancer

Epplein

Pawlita

Michel

et al. 2013

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…Concordance between histology and serology varied between 95% and 100%. A Scandinavian study [61] with an in‐house Western blot assay compared the banding pattern of 115 gastric cancer patients and 110 without cancer and found that cancer patients had significantly more often antibodies to the 94 kDa protein and to the 30 kDa (UreA) as well as a combination of antibodies to the 85 kDa (VacA) and the 120 kDa (CagA).…”

Section: Noninvasive Methodsmentioning

confidence: 99%

Diagnosis of Helicobacter pylori

et al. 2008

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The different invasive and noninvasive diagnostic tests for Helicobacter pylori have been applied mainly in emerging countries. Molecular methods have been developed, especially a test for detection of H. pylori and its clarithromycin resistance directly from stools. The long-term effects of eradication on histologic lesions have been studied in a meta-analysis and the prognostic value of post-treatment in gastric mucosa-associated lymphoid tissue lymphoma has been assessed. An operating link for gastritis assessment (the OLGA staging) has also been published. Attempts to simplify the urea breath test protocol have been made, and new stool antigen tests have been proposed and compared to those previously available.

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“…The 1DE western blotting technique is a very useful tool for seroepidemiological survey, since it detects both former and current H. pylori infection. Exhaustive studies were performed to explore the seroprevalence of the two main H. pylori antigens, CagA and VacA, and their role as risk factors for H. pylori-related gastric diseases [15][16][17]. However, the association between the seroreactivity to these two antigens and the clinical outcomes of the infection is still controversial [18], and varies among geographical regions and ethnic groups [19].…”

Section: Protein Virulence Factors and Diagnosis Of Infectionmentioning

confidence: 99%

Helicobacter pylori: immunoproteomics related to different pathologies

Bernardini¹,

Braconi²,

Lusini³

et al. 2007

Expert Review of Proteomics

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Helicobacter pylori is a Gram-negative bacterium that causes ulcer, atrophic gastritis, adenocarcinoma and mucosa-associated lymphoid tissue lymphoma. Moreover, an ongoing controversial role of this bacterium infection has been suggested in the etiopathogenesis of some extradigestive diseases. The humoral response to H. pylori during a natural infection can be used for diagnostic purposes and as a basis for vaccine development. Host-pathogen interactions may be investigated by means of immunoproteomics, which provides global information about relevant specific and nonspecific antigens, and thus might be suitable to identify novel vaccine candidates or serological markers of H. pylori infection as well as of different related diseases. In this review, we describe how several research groups used H. pylori proteomics combined with western blotting analysis, using sera from patients affected with different H. pylori-related pathologies, to investigate potential associations between host immune response and clinical outcomes of H. pylori infection, resulting in the rapid identification of novel, highly immunoreactive antigens.

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Combined serum IgG response toHelicobacter pyloriVacA and CagA predicts gastric cancer

Cited by 15 publications

References 23 publications

Helicobacter pylori Protein–Specific Antibodies and Risk of Colorectal Cancer

Helicobacter pylori Protein–Specific Antibodies and Risk of Colorectal Cancer

Diagnosis of Helicobacter pylori

Helicobacter pylori: immunoproteomics related to different pathologies

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