Combined surface plasmon resonance and impedance spectroscopy systems for biosensing

Abstract

Combined surface plasmon resonance and electrochemical impedance spectroscopy (SP-EIS) biosensing systems have been developed. They use a sensor chip consisting of micrometer sized interdigitated metal electrodes (IDEs) which effectively act as a surface plasmon supporting structure. In addition, the interdigitated electrodes exhibit the properties of an optical dispersive grating reflecting white light into the diffraction orders. Direct surface plasmon spectral analysis is possible by using the first order of diffraction and has been used as a novel principle for a simplified optical set-up. SP-EIS is a promising technology for a variety of conductometric and SPR mono- and multisensing applications in portable or stationary platforms.

Development of impedimetric DNA biosensor for selective detection and discrimination of oligonucleotide sequences of the rpoB gene of Mycobacterium tuberculosis

“…The possibility of DNA hybridization detection with this marker and without any molecular labels was shown on both gold plate electrodes [15] and interdigitated configurations of the working and auxiliary electrodes [16,17], however, because of a larger working surface area, plate electrodes seems to be the more sensitive.…”

Iterative Precise Conductivity Measurement with IDEs

“…Using comb-like structures of electrodes (interdigitated electrodes—IDEs) with different sizes is still under intensive research as many published articles in the areas of chemical sensing or characterization [ 1 , 2 , 3 , 4 , 5 , 6 ] and biosensing [ 7 , 8 , 9 , 10 , 11 , 12 , 13 ] have shown this year.…”

Trends in aptamer selection methods and applications

“…37 Binding of analytes onto the ligand immobilized on a SPR chip (usually gold) leads an increase in the mass causing a proportional increase in refractive index, which is eventually detected as a shift in the resonance angle. 37 Binding of analytes onto the ligand immobilized on a SPR chip (usually gold) leads an increase in the mass causing a proportional increase in refractive index, which is eventually detected as a shift in the resonance angle.…”