Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus

Ebrahimpour-Malekshah, Roohollah; Amini, Abdollah; Zare, Fatemeh; Mostafavinia, Atarodsadat; Davoody, Samin; Deravi, Niloofar; Rahmanian, Mohammad; Hashemi, Seyed Mahmoud; Habibi, Malihe; Ghoreishi, Seyed Kamran; Chien, Sufan; Shafikhani, Sasha H.; Ahmadi, Houssein; Bayat, Sahar; Bayat, Mohammad

doi:10.1136/bmjdrc-2019-001033

Cited by 41 publications

(51 citation statements)

References 51 publications

(73 reference statements)

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“…However, T2D is developed when the body cannot properly respond to insulin. The most common type of diabetes is T2D, which consists about 85% to 95% of diabetic cases throughout the world (Ebrahimpour-Malekshah et al, 2020;Kulcsar et al, 2019). Etiologically, T2D is highly linked to obesity as well as the outcomes of chronic inflammation caused by excess adipose tissue.…”

Section: The Role Of Diabetes Mellitus and Hypertension In Acute Respmentioning

confidence: 99%

SARS-CoV-2 infection in patients with diabetes mellitus and hypertension: a systematic review

Deravi¹,

Fathi²,

Vakili³

et al. 2020

Rev. Cardiovasc. Med.

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After the emergence of the novel 2019 coronavirus disease in P. R. China, this highly contagious disease has been currently spread out to almost all countries, worldwide. Novel 2019 coronavirus disease, Middle East respiratory syndrome, and severe acute respiratory syndrome are reported to cause a higher risk for severe infections in patients with chronic comorbidities, such as hypertension and diabetes. These severe infections can contribute to higher rates of morbidity and mortality in these patients. In the present review, we discussed the role and underlying mechanisms of the two most common chronic diseases, type-2 diabetes mellitus and hypertension, in clinical manifestations and disease severity of novel 2019 coronavirus disease, Middle East respiratory syndrome and severe acute respiratory syndrome, with the hope to provide evidence for better decision-making in the treatment of this vulnerable population.

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Section: The Role Of Diabetes Mellitus and Hypertension In Acute Respmentioning

confidence: 99%

SARS-CoV-2 infection in patients with diabetes mellitus and hypertension: a systematic review

Deravi¹,

Fathi²,

Vakili³

et al. 2020

Rev. Cardiovasc. Med.

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“…Our results also showed a significant increase in cell viability, corresponding to the results observed in a similar study by de Villiers et al [ 31 ], who observed an increase in cell viability in isolated ADSCs and a commercial ADSC cell line irradiated at a wavelength of 636 nm and a fluence of 5 J/cm 2 . Ebrahimpour-Malekshah et al irradiated diabetic mice with an infected (MRSA) ischemic wound with or without transplanted ADSCs at 890 nm (power 80 W; pulse frequency 80 Hz; pulsed duration 180 ns; duration of exposure 200 s; power density 0.001 W/cm 2 ; and energy density 0.2 J/cm 2 ) 6 days per week for 16 days [ 39 ]. There was a significant decrease in microbial flora and inflammatory response and a significant increase in wound closure rate and strength and angiogenesis in all treated mice; however, it was found that PBM together with transplanted ADSCs showed superior results as compared to PBM and ADSC transplantation alone [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Wound Healing Potential of Photobiomodulation Is Possibly Mediated by Its Stimulatory Effect on AKT Expression in Adipose‐Derived Stem Cells

Rajendran

Houreld

Abrahamse

2021

Oxidative Medicine and Cellular Longevity

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Increasing evidence suggests that adipose-derived stem cells (ADSCs) serve as a therapeutic approach for wound healing. The aim of this study was to determine the effect of photobiomodulation (PBM) on antioxidant enzymes in ADSCs. Four ADSC cell models, namely, normal, wounded, diabetic, and diabetic wounded, were irradiated with 660 nm (fluence of 5 J/cm2 and power density of 11.2 mW/cm2) or 830 nm (fluence of 5 J/cm2 and power density of 10.3 mW/cm2). Nonirradiated cells served as controls. Cell morphology and wound migration were determined using light microscopy. Cell viability was determined by the trypan blue exclusion assay. The enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of antioxidants (superoxide dismutase (SOD), catalase (CAT), and heme oxygenase (HMOX1)). AKT activation and FOXO1 levels were determined by immunofluorescence and western blotting. The gaps (wound) in PBM-treated wounded and diabetic wounded cell models closed faster than the controls. PBM treatment significantly increased antioxidant levels in all cell models. This reflects that oxidative stress is reduced on the counterpart of increased antioxidant levels. This might be due to the activation of the AKT signaling pathway as evidenced by the increased AKT signals via western blotting and immunofluorescence. This data suggests that PBM promotes wound healing by increasing antioxidant levels by activating AKT signaling.

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“…Recently, in our lab, we have engrafted healthy AD-MSC cells into wounds of diabetic rats [23,26]. However, in the current probe, we engrafted preconditioned diabetic AD-MSC with PBM into wounds of diabetic rats.…”

Section: Introductionmentioning

confidence: 90%

“…We photographed the wounds on days 0, 4, 8, 12, and 16 and measured the wound surface area by ImageJ software (NIH, USA). The wound closure rate was calculated as follows [26]. Â Surfacearea at day 0 − surfacearea at day X ð Þ = surfacearea at day 0 Â 100%:…”

Section: Wound Closure Ratementioning

confidence: 99%

Transplantation of photobiomodulation-preconditioned diabetic stem cells accelerates ischemic wound healing in diabetic rats

et al. 2020

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Background Diabetic foot ulcer is the most costly and complex challenge for patients with diabetes. We hereby assessed the effectiveness of different preconditioned adipose-derived mesenchymal stem cells (AD-MSCs) and photobiomodulation protocols on treating an infected ischemic wound in type 1 diabetic rats. Methods There were five groups of rats: (1) control, (2) control AD-MSCs [diabetic AD-MSCs were transplanted (grafted) into the wound bed], (3) AD-MSC + photobiomodulation in vivo (diabetic AD-MSCs were grafted into the wound, followed by in vivo PBM treatment), (4) AD-MSCs + photobiomodulation in vitro, and (5) AD-MSCs + photobiomodulation in vitro + in vivo. Results Diabetic AD-MSCs preconditioned with photobiomodulation had significantly risen cell function compared to diabetic AD-MSC. Groups 3 and 5 had significantly decreased microbial flora correlated to groups 1 and 2 (all, p = 0.000). Groups 2, 3, 4, and 5 had significantly improved wound closure rate (0.4, 0.4, 0.4, and 0.8, respectively) compared to group 1 (0.2). Groups 2–5 had significantly increased wound strength compared to group 1 (all p = 0.000). In most cases, group 5 had significantly better results than groups 2, 3, and 4. Conclusions Preconditioning diabetic AD-MSCs with photobiomodulation in vitro plus photobiomodulation in vivo significantly hastened healing in the diabetic rat model of an ischemic infected delayed healing wound.

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Combined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus

Cited by 41 publications

References 51 publications

SARS-CoV-2 infection in patients with diabetes mellitus and hypertension: a systematic review

SARS-CoV-2 infection in patients with diabetes mellitus and hypertension: a systematic review

In Vitro Wound Healing Potential of Photobiomodulation Is Possibly Mediated by Its Stimulatory Effect on AKT Expression in Adipose‐Derived Stem Cells

Transplantation of photobiomodulation-preconditioned diabetic stem cells accelerates ischemic wound healing in diabetic rats

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