Combined Toxicity of Cannabidiol Oil with Three Bio-Pesticides against Adults of Sitophilus Zeamais, Rhyzopertha Dominica, Prostephanus Truncatus and Trogoderma Granarium

Abstract

The present study investigates the interaction between cannabidiol (CBD) oil and three biopesticides: Azatin and two baculovirus formulations (Madex and Helicovex), both separately and in combination, in order to investigate their interaction against adults of four major coleopteran stored-product pests: Sitophilus zeamais (Coleoptera: Curculionidae), Rhyzopertha dominica (Coleoptera: Bostrichidae), Prostephanus truncatus (Coleoptera: Bostrichidae) and Trogoderma granarium (Coleoptera: Dermestidae). CBD, which has been understudied for its insecticidal properties, was applied at three different doses (500, 1500 and 3000 ppm). The biopesticides were administered at 1500 ppm. Interactions in the combined treatments were mathematically estimated as not synergistic and mostly competitive except for the combined treatments of CBD (1500 and 3000 ppm) with Azatin (1500 ppm) which were marked by an additive interaction. In its individual application, CBD oil generated the highest insect mortality while its effect was clearly dose-dependent. The findings reveal a promising effect of CBD oil against these coleopterans which had not been previously tested together.

Synergistic Field Crop Pest Management Properties of Plant-Derived Essential Oils in Combination with Synthetic Pesticides and Bioactive Molecules: A Review

“…The study further elucidated that these combinative agents induced the inhibition of enzyme pathways associated with oxidase, glutathione S-transferase and general esterase (ά-β-EST) of the tested insect pest [ 104 ]. Mantzoukas et al [ 105 ] stated that the cannabidiol ( Figure 3 ) present in the essential oil of the Cannabis plant synergized the commercially available biopesticides madex, azatin and helicovex against the four crop pests S. zeamais , Rhyzopertha dominica (Fabricius), Prostephanus truncates (Horn) and Trogodermagranarium (Everts) at doses ranging from 500 to 3000 ppm [ 105 ]. Examples of commercially available synthetic pesticides used in combination with plant-derived essential oils and their compounds are summarized in Table 1 .…”

Effect of four plant extracts on the mortality, population growth, and fluctuating asymmetry of Sitophilus oryzae

“…Repellent effects of C. sativa leaf powder have been reported on S. oryzae by several authors (Khare et al, 1974;Prakash et al, 1982). Additionally, cannabidiol oil expressed insecticidal effects on adult S. zeamais and three other stored product pests [Rhyzopertha dominica (Fabricius), Prostephanus truncatus (Horn), and Trogoderma granarium Everts] in a recent study by Mantzoukas et al (2020). However, Cannabis treatment can also have positive effects on insects; and as presented by Park et al (2019), it significantly enhanced ethanol-intoxicated Manduca sexta (L.) larval survival by 40%.…”

The Synergistic Effect of Lemongrass Essential Oil and Flometoquin, Flonicamid, and Sulfoxaflor on Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae): Insights into Toxicity, Biochemical Impact, and Molecular Docking

“…Nevertheless, chemical mixtures, particularly those which combine EOs with low doses of chemical insecticides, are viewed as a promising strategy for insect resistance management (IRM) [12]. There are several studies that have evaluated mixtures of EOs and insecticides [44][45][46][47][48][49]. However, the current study represents the initial exploration of the impact of lemongrass EO combined with different pesticides on the metabolic enzymes in Bemisia tabaci adults.…”