Combined Transcriptome and Proteome Analysis of Anthers of AL-type Cytoplasmic Male Sterile Line and Its Maintainer Line Reveals New Insights into Mechanism of Male Sterility in Common Wheat

Abstract

Cytoplasmic male sterility (CMS) plays an essential role in hybrid seeds production. In wheat, orf279 was reported as a CMS gene of AL-type male sterile line (AL18A), but its sterility mechanism is still unclear. Therefore, transcriptomic and proteomic analyses of the anthers of AL18A and its maintainer line (AL18B) were performed to interpret the sterility mechanism. Results showed that the electron transport chain and ROS scavenging enzyme expression levels changed in the early stages of the anther development. Biological processes, i.e., fatty acid synthesis, lipid transport, and polysaccharide metabolism, were abnormal, resulting in pollen abortion in AL18A. In addition, we identified several critical regulatory genes related to anther development through combined analysis of transcriptome and proteome. Most of the genes were enzymes or transcription factors, and 63 were partially homologous to the reported genic male sterile (GMS) genes. This study provides a new perspective of the sterility mechanism of AL18A and lays a foundation to study the functional genes of anther development.

Combined analysis of transcriptome and metabolome reveals that sugar, lipid, and phenylpropane metabolism are essential for male fertility in temperature-induced male sterile rice

“…Some studies have found that in the genic male sterile line land cotton, TPP and TPS proteins in trehalose biosynthesis are significantly downregulated in sterile anthers; in addition, nine trehalose synthesis-related genes were differentially expressed in differentially fertile anthers of wheat. Therefore, it is believed that abnormal synthesis of trehalose causes pollen abortion ( Yue et al, 2014 ; Li et al, 2019b ; Hao et al, 2021a ). In Arabidopsis , loss of function of AtTPS1 also leads to altered cell wall structure and embryonic lethality ( Gómez et al, 2006 ).…”

Integrated transcriptomic and proteomic analysis of a cytoplasmic male sterility line and associated maintainer line in soybean

“…To elucidate the molecular mechanism underlying CMS, transcriptomic and proteomic approaches have been applied in many higher plants, such as Brassica napus ( Yan et al., 2013 ; Ning et al., 2019 ), Brassica campestris ( Chen et al., 2018 ), Capsicum annuum ( Guo et al., 2017 ), Gossypium hirsutum ( Suzuki et al., 2013 ), Zea mays ( Zhang et al., 2020 ), and Triticum aestivum ( Hao et al., 2021 ). Transcriptomic and proteomic analyses of Brassica napus Shaan2A anther mitochondria implied that the sterility gene may suppress expression of several nuclear transcription factors (TFs), affecting early anther development ( Ning et al., 2019 ).…”

Comparative Transcriptome Analysis Reveals a Potential Regulatory Network for Ogura Cytoplasmic Male Sterility in Cabbage (Brassica oleracea L.)

“…In recent years, with the development of high-throughput sequencing technology and bioinformatics, an increasing number of researchers have explored the molecular mechanism of CMS through comparative analysis of the transcriptomes, proteomes, or metabolomes. A combined analysis of the transcriptomes and proteomes between wheat AL-CMS and its maintainers revealed that most of the key regulatory genes related to anther development were enzymes or transcription factors (TFs) [ 31 ]. In addition, a transcriptome analysis of wheat K-TCMS showed that genes related to phenylpropane biosynthesis and jasmonic acid biosynthesis were upregulated in CMS anthers and regulated by MYB TFs [ 32 ].…”