Combined transcriptomic and metabolomic analyses of high temperature stress response of quinoa seedlings

Abstract

Background Quinoa (Chenopodium quinoa Willd.) originates in high altitude areas, such as the Andes, and has some inherent characteristics of cold, drought, and salinity tolerance, but is sensitive to high temperature. Results To gain insight into the response mechanism of quinoa to high temperature stress, we conducted an extensive targeted metabolomic study of two cultivars, Dianli-3101 and Dianli-3051, along with a combined transcriptome analysis. A total of 794 metabolites and 54,200 genes were detected, in which the genes related to photosynthesis were found down-regulated at high temperatures, and two metabolites, lipids and flavonoids, showed the largest changes in differential accumulation. Further analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and transcription factors revealed that quinoa inhibits photosynthesis at high temperatures, and the possible strategies being used for high temperature stress management are regulation of heat stress transcription factors (HSFs) to obtain heat tolerance, and regulation of purine metabolism to enhance stress signals for rapid response to high temperature stress. The tolerant genotype could have an enhanced response through lower purine levels. The induction of the stress response could be mediated by HSF transcription factors. The results of this study may provide theoretical references for understanding the response mechanism of quinoa to high temperature stress, and for screening potential high temperature tolerant target genes and high temperature tolerant strains. Conclusions These findings reveal the regulation of the transcription factor family HSF and the purinergic pathway in response to high temperature stress to improve quinoa varieties with high temperature tolerance.

Transcriptome sequencing of garlic reveals key genes related to the heat stress response

“…Heat shock protein (HSP) acts as a molecular chaperone to assist protein refolding, stabilization, intracellular transport, and degradation, prevent the accumulation of damaged proteins, and maintain the stability of the intracellular environment 31 , 32 . High-temperature stress transcripome analysis of quinoa showed that HSF transcription factors such as LOC110702486 , LOC110697083 , LOC110729384 , LOC110709409 , and LOC11073445 were the core regulatory factors of transcription under high temperatures 33 . When plants are subjected to high-temperature stress, HSP accumulates a large amount of expression in a short time and participates in the stress response, but with the increase in treatment time, the stress response is weakened and HSP is degraded 5 .…”

Crop Resistance Mechanisms to Alleviate Climate Change-Related Stress

“…To safeguard cells from stress-induced damage, plants adapt by reprogramming their metabolic pathways. While there are several metabolome datasets under individual drought and heat stress (Ye et al, 2016;Sun et al, 2019;Abdelrahman et al, 2020;Lecourieux et al, 2020;Guo et al, 2021;Lu et al, 2022;Loṕez et al, 2023;Xie et al, 2023), there are only a few studies with respect to combined stress (Zinta et al, 2018;Alhaithloul et al, 2019;Lawas et al, 2019;Qaseem et al, 2019;Xie et al, 2020;Yousaf et al, 2022;Ru et al, 2023). Under combined stress, the processes of carbohydrate metabolism, amino acid metabolism and organic acid are differentially affected compared with a non-stress condition (Zinta et al, 2018;Lawas et al, 2019).…”

When drought meets heat – a plant omics perspective

“…To safeguard cells from stress-induced damage, plants adapt by reprogramming their metabolic pathways. While there are several metabolome datasets under individual drought and heat stress ( Ye et al., 2016 ; Sun et al., 2019 ; Abdelrahman et al., 2020 ; Lecourieux et al., 2020 ; Guo et al., 2021 ; Lu et al., 2022 ; López et al., 2023 ; Xie et al., 2023 ), there are only a few studies with respect to combined stress ( Zinta et al., 2018 ; Alhaithloul et al., 2019 ; Lawas et al., 2019 ; Qaseem et al., 2019 ; Xie et al., 2020 ; Yousaf et al., 2022 ; Ru et al., 2023 ). Under combined stress, the processes of carbohydrate metabolism, amino acid metabolism and organic acid are differentially affected compared with a non-stress condition ( Zinta et al., 2018 ; Lawas et al., 2019 ).…”