Combined transcriptomic and proteomic analysis of flubendiamide resistance in Plutella xylostella

Abstract

Diamondback moth (DBM), Plutella xylostella, is an important pest of crucifers worldwide. The extensive use of diamide insecticides has led to DBM resistance in the world, and this presents a serious threat to vegetable production. In the present study, transcriptomic and proteomic analyses were combined to investigate the potential flubendiamide-resistance mechanism in DBM. The lab-selected (R h) and field-collected (R b) flubendiamide-resistant lines of P. xylostella with resistance ratios of 1889.92-fold and 1250.97-fold, respectively, were used, as well as a lab-reared flubendiamide-susceptible line (S). Compared with the S group, the transcriptomic analysis revealed 151 upregulated and 287 downregulated gene messengers in the R h group and 432 upregulated and 565 downregulated gene messengers in the R b group. The most frequently enriched pathways of differentially expressed genes (DEGs) were mainly involved in metabolic pathways. Metabolism related genes, including two P450, two ABC transporters, and three trypsins, were upregulated in the R h line. Additionally, some P450 genes, trypsin, juvenile hormone (JH), and mucin genes were also upregulated in the R b line. In proteomic analysis comparisons with the S group, there were 78 upregulated and 90 downregulated proteins in the R h group and 221 upregulated and 155 downregulated proteins in the R b group. Further analyses found that three CYP and 11 CYP proteins were over-expressed in R h and R b lines, respectively. Four glutathione S-transferase (GST) and four UGTs were over-expressed in R b line. So, we deduced that the detoxification metabolism may be the main mechanism of flubendiamide resistance in P. xylostella.

Novel Evodiamine-Based Sulfonamide Derivatives as Potent Insecticide Candidates Targeting Insect Ryanodine Receptors

“…8−14 For example, the diamondback moth, Plutella xylostella Linnaeus (Lepidoptera: Plutellidae), is among the most destructive agricultural pests that have developed severe resistance to multiple classes of insecticide. 15,16 Consequently, there is an urgent need for the identification of efficacious alternatives for pest control.…”

“…With the steady expansion of the global population, the future trajectory of agricultural development is increasingly oriented toward environmentally friendly, efficient, health-conscious, and sustainable practices. , Among the various challenges faced, the menace of pests emerges as a main factor impeding efficient agricultural output and jeopardizing global food security. , While a repertoire of pest-controlling measures, including genetically modified (GM) crops and biopesticides, have been introduced and applied successively, traditional chemical insecticides remain the predominant method employed in modern agriculture to protect and safeguard crops from pests at the current stage. , Nevertheless, the excessive and unregulated use of traditional chemical insecticides often leads to environmental contamination, ecological imbalances, and resistance development among pests. − For example, the diamondback moth, Plutella xylostella Linnaeus (Lepidoptera: Plutellidae), is among the most destructive agricultural pests that have developed severe resistance to multiple classes of insecticide. , Consequently, there is an urgent need for the identification of efficacious alternatives for pest control.…”

Design, synthesis and biological activity of diamide compounds based on 3‐substituent of the pyrazole ring†

“…However, due to the wide application of diamide insecticides and lack of rotation, insects have rapidly developed high resistance to the diamide insecticides by evolving mutations in their RyRs. In some cases, the resistance of agricultural insects, such as Plutella xylostella (diamondback moth, DBM), has increased more than 2000 times 8,9 . The diamide insecticides have also caused some ecological problems, including environmental pollution, toxicity to some aquatic organisms and honeybees 10 .…”

The glutathione S-transferase (PxGST2L) may contribute to the detoxification metabolism of chlorantraniliprole in Plutella xylostella(L.)

“…DBM develops resistance to insecticides very rapidly due to its physiological and biochemical characteristics. At present, DBM has developed resistance to all major classes of insecticides (Arruda et al 2020;Charleston et al 2005;Jing et al 2021;Li et al 2020;Pu et al 2010;Wang et al 2019;Zhao et al 2006). The lack of effective insecticides makes it difficult to prevent and control DBM.…”

“…Modified acetylcholinesterase (AChE) phenotype (MACE) caused by a mutation in the ACHE gene (ace2) is strongly involved in the resistance of Myzus persicae (Sulzer) (Homoptera: Aphididae) to pirimicarb (Liu et al 2017). Most studies on the mechanisms underlying resistance in DBM have focused on genetic and physiological changes and have reported that mutation of RYR and detoxification metabolism as the two main causes (He et al 2012;Li et al 2020;Wang et al 2020). Transport protein ABCC2 participates in the resistance of DBM to avermectin and chlorfenapyr (Xu et al 2019).…”