Combined Transcriptomics and Metabolomics of Arabidopsis thaliana Seedlings Exposed to Exogenous GABA Suggest Its Role in Plants Is Predominantly Metabolic

Batushansky, Albert; Kirma, Menny; Grillich, Nicole; Toubiana, David; Pham, Phuong Anh; Balbo, Ilse; Fromm, Hillel; Galili, Gad; Fernie, Alisdair R.; Fait, Aaron

doi:10.1093/mp/ssu017

Cited by 65 publications

(52 citation statements)

References 13 publications

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“…Further oxidative stress leads to impaired GABA-shunt thus increasing plant’s susceptibility to oxidative stressors such as drought and heavy metals37. Taking into consideration that GABA shunt bypass TCA cycle4143 and that the increased level of GABA can suppress TCA cycle intermediates like citrate and malate under C deficiency44 we can suggest a shift of C skeletons flow from sugars metabolism to TCA cycle (Fig. 3).…”

Section: Resultsmentioning

confidence: 99%

Paclobutrazol induces tolerance in tomato to deficit irrigation through diversified effects on plant morphology, physiology and metabolism

Pal

Zhao

Khan

et al. 2016

Sci Rep

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Dwindling water resources combined with meeting the demands for food security require maximizing water use efficiency (WUE) both in rainfed and irrigated agriculture. In this regard, deficit irrigation (DI), defined as the administration of water below full crop-water requirements (evapotranspiration), is a valuable practice to contain irrigation water use. In this study, the mechanism of paclobutrazol (Pbz)-mediated improvement in tolerance to water deficit in tomato was thoroughly investigated. Tomato plants were subjected to normal irrigated and deficit irrigated conditions plus Pbz application (0.8 and 1.6 ppm). A comprehensive morpho-physiological, metabolomics and molecular analysis was undertaken. Findings revealed that Pbz application reduced plant height, improved stem diameter and leaf number, altered root architecture, enhanced photosynthetic rates and WUE of tomato plants under deficit irrigation. Pbz differentially induced expression of genes and accumulation of metabolites of the tricarboxylic acid (TCA) cycle, γ-aminobutyric acid (GABA-shunt pathway), glutathione ascorbate (GSH-ASC)-cycle, cell wall and sugar metabolism, abscisic acid (ABA), spermidine (Spd) content and expression of an aquaporin (AP) protein under deficit irrigation. Our results suggest that Pbz application could significantly improve tolerance in tomato plants under limited water availability through selective changes in morpho-physiology and induction of stress-related molecular processes.

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Section: Resultsmentioning

confidence: 99%

Paclobutrazol induces tolerance in tomato to deficit irrigation through diversified effects on plant morphology, physiology and metabolism

Pal

Zhao

Khan

et al. 2016

Sci Rep

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“…This thesis is supported by Renault et al [41] findings, who observed growth inhibition of primary roots and dark-grown hypocotyl in A. thaliana as the result of decreased expression of the genes encoding secreted and cell wall-related proteins. Also Batushansky et al [42] showed that expression of genes associated with cell wall was inhibited in seedlings of Arabidopsis thaliana by exogenous GABA.…”

Section: Discussionmentioning

confidence: 99%

Putrescine catabolism via DAO contributes to proline and GABA accumulation in roots of lupine seedlings growing under salt stress

et al. 2017

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The levels of polyamines (PAs), proline (Pro), and γ-aminobutyric acid (GABA) as well as the activity of diamine oxidase (DAO; EC 1.4.3.6) were studied in the roots of 2-day-old lupine (Lupinus luteus L. 'Juno') seedlings treated with 200 mM NaCl for 24 h. The effect of adding 1 mM aminoguanidine (AG), an inhibitor of DAO activity, was also analyzed. It was found that in roots of lupine seedlings growing under salt stress, a negative correlation between Pro accumulation and putrescine (Put) content takes place. Pro level increased in roots by about 160% and, at the same time, Put content decreased by about 60%, as a result of ca. twofold increase of DAO activity. The AG added to the seedlings almost totally inhibited the activity of DAO, increased Put accumulation to control level, decreased Pro content by about 25%, and reduced GABA level by about 22%. Addition of 50 mM GABA to the lupine seedlings growing in the presence of AG and NaCl restored Pro content in roots to its level in NaCl-treated plants. In this research, the clear correlation between Put degradation and GABA and Pro accumulation was shown for the first time in the roots of seedlings growing under salt stress. This could be considered as a short-term response of a plant to high salt concentration. Our findings indicate that during intensive Pro accumulation in roots induced by salt stress, the pool of this amino acid is indirectly supported by GABA production as a result of Put degradation.

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“…Molina-Rueda et al (2015) proposed a model for GABA in vascular differentiation, defense and signaling in pine. After their extensive transcriptomic and metabolomic profiling in A. thaliana, Batushansky et al (2014) pointed out GABA to have a role in the central N-metabolism which was more remarkable under C-limiting conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Different functions have been attributed to GABA such as; a regulator of cytosolic pH, a signaling molecule, a modulator of C-N balance, a compatible solute, a plant defense component and a regulator of growth and development. (Shelp et al, 1999;Batushansky et al, 2014;MolinaRueda et al, 2015). The main route in GABA biosynthesis is the conversion of glutamate to GABA by the activity of glutamate decarboxylase (GAD, EC 4.1.1.15), an enzyme localized in the cytosol (Bown and Shelp, 1997;Shelp et al, 1999;Bouche and Fromm, 2004).…”

Section: Introductionmentioning

confidence: 99%

The impact of GABA in harpin-elicited biotic stress responses in Nicotiana tabaccum

Dimlioğlu

Daş

Bor

et al. 2015

Journal of Plant Physiology

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Combined Transcriptomics and Metabolomics of Arabidopsis thaliana Seedlings Exposed to Exogenous GABA Suggest Its Role in Plants Is Predominantly Metabolic

Cited by 65 publications

References 13 publications

Paclobutrazol induces tolerance in tomato to deficit irrigation through diversified effects on plant morphology, physiology and metabolism

Paclobutrazol induces tolerance in tomato to deficit irrigation through diversified effects on plant morphology, physiology and metabolism

Putrescine catabolism via DAO contributes to proline and GABA accumulation in roots of lupine seedlings growing under salt stress

The impact of GABA in harpin-elicited biotic stress responses in Nicotiana tabaccum

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