Combined treatment of nonresectable cholangiocarcinoma complicated by obstructive jaundice

Shiryaev, Artem; Musaev, G. Kh.; Levkin, V.V.; Reshetov, Igor V.; Loshchenov, Maxim; Alekseeva, Polina; Волков, В. В.; Linkov, Kirill G.; Makarov, Vladimir; Shchekoturov, I.O.; Borodkin, A. V.; Loschenov, Viktor

doi:10.1016/j.pdpdt.2019.04.006

Cited by 17 publications

(11 citation statements)

References 13 publications

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“…Высокая фототоксичность РХ [8], хорошая избирательность накопления препарата в опухоли и быстрое выведение его из организма обусловливают отсутствие гиперфоточувствительности кожных покровов и малое количество побочных системных эффектов после ФДТ [8]. Эффективность ФДТ с использованием РХ показана для злокачественных новообразований кожи, легких, желудочно-кишечного тракта, мочеполовых путей [9,10].…”

Section: Introductionunclassified

Development of Polymeric Microparticles With Radachlorine and Estimation of the Prospects of Their Use in Photodynamic Therapy

Miroshkina

Krechetov

Solovieva

et al. 2021

Rossijskij bioterapevtičeskij žurnal

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Introduction. Nowadays, the development of delivery systems based on micro- and nanoparticles is being actively pursued to increase the selectivity and efficiency of photosensitizers in photodynamic therapy. Such microparticles could increase the effectiveness of the already used chemotherapeutic drugs due to their accumulation in the tumor and help to overcome the drug resistance of tumor cells.The aim of this research was to obtain microparticles based on a biocompatible block copolymer of lactic and glycolic acids with the inclusion of the photosensitizer radachlorin, magnetic nanoparticles, and perfluorodecalin and their subsequent evaluation as therapeutic agents for photodynamic therapy.Materials and methods. Microparticles were obtained using the double emulsion method, described using of electron microscopy. Evaluation of their photodynamic properties was carried out using spectrophotometry and MTTtest on cell culture.Results. Spherical microparticles with a size of less than 1 μm were obtained. The release of the active substance from microparticles occurred gradually over two weeks, and in the case of the presence of magnetic nanoparticles, the concentration of radachlorin remained practically unchanged for a month. Exposure of microparticles to the light of LED is accompanied by the formation ofsinglet oxygen. Electron microscopy indicated intracellular position of microparticlesin tumor cells. The MTT test revealed a significant inhibition of cell viability in the presence of microparticles.Conclusion. The research results allow us to consider the obtained biocompatible polymer microparticles with the inclusion of radachlorin as a depot of radachlorin for local use in photodynamic therapy of tumors.

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Section: Introductionunclassified

Development of Polymeric Microparticles With Radachlorine and Estimation of the Prospects of Their Use in Photodynamic Therapy

Miroshkina

Krechetov

Solovieva

et al. 2021

Rossijskij bioterapevtičeskij žurnal

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“…18,20 The efficiency of PDT using RC is shown for malignant neoplasms of the skin, lungs, gastrointestinal tract, and genitourinary tract. [21][22][23] The presence of inherent fluorescence in RC, when exposed to light, used in PDT, 19 makes it possible to use this PS for both PDT and fluorescent diagnostics. 22,23 The inclusion of RC into MPs is considered as a promising trend for improving the efficiency of its use for PDT and fluorescence diagnostics.…”

Section: Introductionmentioning

confidence: 99%

“…[21][22][23] The presence of inherent fluorescence in RC, when exposed to light, used in PDT, 19 makes it possible to use this PS for both PDT and fluorescent diagnostics. 22,23 The inclusion of RC into MPs is considered as a promising trend for improving the efficiency of its use for PDT and fluorescence diagnostics. 24 The preparation of such MPs should potentiate intracellular accumulation and deposition of RC, as well as prolongation of its effect.…”

Section: Introductionmentioning

confidence: 99%

“… 18 , 20 The efficiency of PDT using RC is shown for malignant neoplasms of the skin, lungs, gastrointestinal tract, and genitourinary tract. 21 - 23 The presence of inherent fluorescence in RC, when exposed to light, used in PDT, 19 makes it possible to use this PS for both PDT and fluorescent diagnostics. 22 , 23 …”

Section: Introductionmentioning

confidence: 99%

“… 21 - 23 The presence of inherent fluorescence in RC, when exposed to light, used in PDT, 19 makes it possible to use this PS for both PDT and fluorescent diagnostics. 22 , 23 …”

Section: Introductionmentioning

confidence: 99%

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Radachlorin-Containing Microparticles for Photodynamic Therapy

et al. 2020

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Purpose: Reducing the undesirable systemic effect of photodynamic therapy (PDT) can be achieved by incorporating a photosensitizer in microparticles (MPs). This study is devoted to the preparation of biocompatible biodegradable MPs with the inclusion of the natural photosensitizer Radachlorin (RС) and an assessment of the possibility of their use for PDT. Methods: RC-containing MPs (RС MPs) with poly(lactic-co-glycolic acid) copolymer (PLGA) matrix were prepared by a double emulsion solvent evaporation methods. The size and morphology of RC MPs were surveyed using scanning electron microscopy, confocal laser scanning microscopy, and dynamic light scattering. The content of RC, its release from RC MPs, and singlet oxygen generation were evaluated by the optical spectroscopy. Cellular uptake and cytotoxic photodynamic effect of RC MPs were investigated with in vitro assays. Results: The average diameter of the prepared RC MPs was about 2-3 μm. The RC MPsprepared by the water/oil/oil method had a significantly higher inclusion of RC (1.74 μg/mg) then RC MPs prepared by the water/oil/water method (0.089 μg/mg). Exposure of the prepared RC MPs to PDT light radiation was accompanied by the singlet oxygen generation and a cytotoxic effect for tumor cells. The release of the RC from the RC MPs was prolonged and lasted at least two weeks. Conclusion: PLGA RC MPs were found to cause a photoactivated cytotoxic effect for tumor cells and can be used for local application in PDT of tumors.

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Phonozen-mediated photodynamic therapy comparing two wavelengths in a mouse model of peritoneal carcinomatosis

Kim,

Lee,

Shin

et al. 2023

Photochem Photobiol Sci

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Combined treatment of nonresectable cholangiocarcinoma complicated by obstructive jaundice

Cited by 17 publications

References 13 publications

Development of Polymeric Microparticles With Radachlorine and Estimation of the Prospects of Their Use in Photodynamic Therapy

Development of Polymeric Microparticles With Radachlorine and Estimation of the Prospects of Their Use in Photodynamic Therapy

Radachlorin-Containing Microparticles for Photodynamic Therapy

Phonozen-mediated photodynamic therapy comparing two wavelengths in a mouse model of peritoneal carcinomatosis

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