Combined treatment with a traditional Chinese medicine, Hachimi-jio-gan (Ba-Wei-Di-Huang-Wan) and alendronate improves bone microstructure in ovariectomized rats

Chen, Huayue; Wu, Minjie; Kubo, Kin‐ya

doi:10.1016/j.jep.2012.04.017

Cited by 19 publications

(7 citation statements)

References 32 publications

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“…The first lumbar vertebra, which was harvested 150 days after the bilateral ovariectomy (day 0 of treatment) and 60 days or 120 days after treatment was cleaned of adherent soft tissues and preserved in sealed plastic bags at −20°C prior to the assay ( 34 ). The MicroCT examination of the first lumbar vertebra was performed using a MicroCT system ( μ CT 80, SCANCO Medical, Brüttisellen, Switzerland) as previously described ( 34 , 35 ), and the analytical conditions were 55 kV with 72 μ A leakage. The lumbar vertebra was scanned and ~500 transverse consecutive sections of 35- μ m thickness were obtained from each lumbar vertebra using a 2048×2048 matrix.…”

Section: Methodsmentioning

confidence: 99%

Effects of OsteoKing on osteoporotic rabbits

Dai

et al. 2015

Molecular Medicine Reports

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Heng-Gu-Gu-Shang-Yu-He-Ji, also known as OsteoKing, is used as a herbal Traditional Chinese Medicine for the treatment of bone disease, including femoral head necrosis and osteoarthritis. However, whether OsteoKing has anti-osteoporotic properties has remained to be elucidated. The purpose of the present study was therefore to investigate the effects of OsteoKing on ovariectomy-induced osteoporosis in rabbits. Female New Zealand white rabbits were randomly divided into an ovariectomized (OVX) group and a sham-surgery group. The rabbits in the OVX group were subjected to an ovariectomy, while the rabbits in the sham group were subjected to the removal of an area of fat near the two ovaries. Bone mineral density, mechanical properties, serum biochemical parameters and micro-architecture were examined at 150 days post-OVX to characterize the experimental animal model. Once the osteoporotic rabbit model had been established, the rabbits in the OVX group were divided into the following groups: Model group, nilestriol group and 300 and 600 mg/kg OsteoKing groups, containing 16 rabbits in each group. OsteoKing and nilestriol were administered orally. The bone mineral density, mechanical properties, serum biochemical parameters, histology and micro-architecture were examined using dual-energy X-ray absorptiometric analysis, mechanical assessments, enzyme-linked immunosorbent assays, histopathological evaluation and micro-computerized tomography examination following 60 days and 120 days of treatment, respectively. Treatment with OsteoKing led to an elevation in the bone mineral density of the vertebra and serum phosphorus levels, reduced serum concentrations of osteocalcin, procollagen type I N-terminal peptide, tartrate-resistant acid phosphatase 5b and cross-linked N-telopeptide of type I collagen, improved mechanical properties (maximum load, stiffness and energy absorption capacity), and micro-architecture of the lumbar vertebra in the OVX osteoporotic rabbit model following treatment for 120 days. In conclusion, it was demonstrated that OsteoKing is effective in the prevention of estrogen deficiency-associated bone loss and may be a promising drug for the treatment of post-menopausal osteoporosis.

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Section: Methodsmentioning

confidence: 99%

Effects of OsteoKing on osteoporotic rabbits

Dai

et al. 2015

Molecular Medicine Reports

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“…Osteoporosis is characterized by decreased bone mass and altered microarchitecture with a consequent increase in bone frailty and susceptibility to fractures [1][2][3][4]. The incidence of osteoporosis commonly occurs in postmenopausal women due to estrogen deficiency, as an imbalance between osteoclastic bone resorption and osteoblastic bone formation.…”

Section: Introductionmentioning

confidence: 99%

Kefir improves bone mass and microarchitecture in an ovariectomized rat model of postmenopausal osteoporosis

et al. 2014

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“…To the best of our knowledge, this is the first study in which the efficacy of BJHD, a TCM, in the treatment of DOP has been explored. The effects of TCMs such as Er‐Zhi‐Wan, Er‐Xian decoction, and Ba‐Wei‐Di‐Huang‐Wan on osteoporosis have been explored, mainly in ovariectomy (OVX)‐induced osteoporosis and they all showed definite anti‐osteoporotic effects. However, the effect of TCM on DOP has not been studied.…”

Section: Discussionmentioning

confidence: 99%