Combined treatment with niacin and chromium caused a protective effect on the small-intestine tissue of hyperlipidemic rats

Abstract

The present study investigated whether a combined treatment of niacin and chromium (III) chloride caused a protective effect on the intestine of hyperlipidemic rats. Rats were divided into four groups: (I) rats were fed with pellet chow, (II) with a lipogenic diet for 60 days, (III) with a lipogenic diet and treated with 100 mg/kg niacin and 250 lg/kg chromium chloride for 45 days by the gavage technique after experimental animals had became hyperlipidemic (15 days), and (IV) with pellet chow and treated with the same dose of niacin and chromium chloride for the last 45 days. Hyperlipidemic rats displayed a reduction of the intestinal glutathione level and an increase in intestinal lipid peroxidation levels, and in serum cholesterol, total lipid and phospholipids levels. However, treatment with niacin and chromium reversed the effect of hyperlipidemia. Light microscopy revealed compressed villi, oedema, mononuclear cell filtration, and vacuolization on the cytoplasm of epithelial cells in the jejunum of hyperlipidemic rats. Ultrastructurally, the disruption of arrays of rough endoplasmic reticulum cisternae, an increase and gathering in smooth endoplasmic reticulum, swelling in mitochondrial cristae and lipid vacuoles were detected in epithelial cells of hyperlipidemic rat jejunum. Despite individual differences, degenerative findings were decreased in hyperlipidemic rats given niacin and chromium. Niacin and chromium treatments had a protective effect on the intestine of the hyperlipidemic rats as well as a lipidreducing effect.

Histopathological alterations in organ structures of hypercholesterolemic rats fed Ortanique peel polymethoxylated flavones

“…The small intestinal changes observed in our study (increased villus: crypt length and absorptive cell: goblet cell ratios) have been previously documented in other studies 34 . These changes may be due to the cell proliferative activity of cholesterol in untreated hypercholesterolemic rats 35 .…”