Combined use of a specific probe and PCAT medium to study Burkholderia in soil

Pallud, Céline; Viallard, Véronique; Balandreau, J.; Normand, Philippe

doi:10.1016/s0167-7012(01)00287-1

Cited by 27 publications

(19 citation statements)

References 34 publications

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“…This observation indicates that although transmission of these bacteria is significant in the colonization of CF patients, in a CF center that follows the recommended control measures, as is the case at the Lisbon CF center, other primary sources of infection must account for many of the cases. It is likely that the environment may act as reservoir for novel BCC infections (14,20,24). This study supports the recommendation for the exclusive use of sterile saline solutions by CF patients.…”

Section: Vol 45 2007 Notes 1631supporting

confidence: 82%

Exceptionally High Representation of Burkholderia cepacia among B. cepacia Complex Isolates Recovered from the Major Portuguese Cystic Fibrosis Center

et al. 2007

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Burkholderia cepacia , a species found infrequently in cystic fibrosis (CF), was isolated from 85% of patients infected with bacteria of the B. cepacia complex that visited the major Portuguese CF center, in Lisbon, during 2003 to 2005. A detailed molecular analysis revealed that this was mainly due to two B. cepacia clones. These clones were indistinguishable from two strains isolated from intrinsically contaminated nonsterile saline solutions for nasal application, detected during routine market surveillance by the Portuguese Medicines and Health Products Authority.

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Section: Vol 45 2007 Notes 1631supporting

confidence: 82%

Exceptionally High Representation of Burkholderia cepacia among B. cepacia Complex Isolates Recovered from the Major Portuguese Cystic Fibrosis Center

et al. 2007

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“…since some gamma-proteobacteria episodically grew in this medium (Pallud et al, 2001). Species abundance and diversity of B. cepacia complex in the environment was studied by Ramette et al (2005) in which TB-T was chosen over other B. sp.…”

Section: Resultsmentioning

confidence: 99%

Studies on endophytic Burkholderia sp. from sugarcane and its screening for plant growth promoting potential

Arthee¹,

Marimuthu²

2017

JEBAS

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KEYWORDS Burkholderia MALDI-TOF MS Plant growth promotion Phylogeny ABSTRACTThe occurrence of Burkholderia genus as plant endophyte indigenous to locally cultivated sugarcane crop was studied. Totally 22 endophytic isolates were obtained from TB-T medium plates of which 10 were found to be putatively endophytic in sugarcane. The morphological, biochemical and MALDI-TOF MS analysis were done and 5 endophytic Burkholderia sp. isolates were identified. Among them, the isolate Burkholderia sp. ES4 shown maximum; diazotrophic activity (total nitrogen: 11.9 mg g -1 of malate; nitrogenase activity: 578.3 n mol C2H4 released h -1 mg -1 protein), mineral solubilization activity (10.2mm, 8.2mm and 3.2 mm solublization zone of P, ZnO and CaCO3 respectively; available phosphorous: 0.72mg ml -1 ), antagonistic activity (54.56 percent inhibition of Colletotrichum falcatum mycelium) and phytohormone production (IAA (16.09 µg ml -1 ); GA (10.54 µg ml -1 )), thereby revealing its plant growth promoting potential. The 16S r RNA gene sequence of the isolate Burkholderia sp. ES4 was sequenced and its phylogenetic analysis shown that the isolate forms a monophyletic subclade within the plant-beneficial-environment" (PBE) Burkholderia group. Thus a potent native plant endophytic strain, Burkholderia sp. ES4 that could be developed as biofertilizer inoculum for sugarcane crop, was obtained in this study.

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“…grew on PCAT, while Ralstonia strains did not grow. This medium is not completely specific for Burkholderia and some gamma-proteo bacteria episodically grow in it (Pallud et al, 2001). Conversely there are a few strains of Burkholderia, which are unable to grow on PCAT, but it is exceptional and limited to a few collection strains.…”

Section: Sugarcane Field Trialsmentioning

confidence: 99%

Improved Yield of Micropropagated Sugarcane Following Inoculation by Endophytic Burkholderia vietnamiensis

et al. 2006

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During a survey of nitrogen-fixing Burkholderia associated with sugarcane in Tamil Nadu, some endophytes were isolated on PCAT medium. Isolation was based on the use of the selective PCAT medium. Four isolates were studied, all belonging to the genus Burkholderia. One of them, MG43 was consistently more active in reducing acetylene and was identified as Burkholderia vietnamiensis. This isolate was used to inoculate micro-propagated sugarcane plantlets in a comparison with two other diazoptrophs, viz. Gluconacetobacter diazotrophicus T and Herbaspirillum seropedicae T . Inoculated plants and uninoculated controls were used in a pot experiment followed by two field experiments under different rates of nitrogen fertilisers. MG43 and G. diazotrophicus performed best in sugarcane, their natural host. Biomass increase due to MG43 inoculation reached 20% in the field. Inoculated plants were heavily colonised by the inoculated bacterium (up to 115,000 CFU g )1 root fresh weight). Inoculation by a combinaison of the three strains performed less well than inoculation by a single MG43 suspension. Ecological implications are discussed, as well as the potential of these bacteria to provide a feasible alternative to higher N fertilisers rates in a low input and long term sustainable rural economy.

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Combined use of a specific probe and PCAT medium to study Burkholderia in soil

Cited by 27 publications

References 34 publications

Exceptionally High Representation of Burkholderia cepacia among B. cepacia Complex Isolates Recovered from the Major Portuguese Cystic Fibrosis Center

Exceptionally High Representation of Burkholderia cepacia among B. cepacia Complex Isolates Recovered from the Major Portuguese Cystic Fibrosis Center

Studies on endophytic Burkholderia sp. from sugarcane and its screening for plant growth promoting potential

Improved Yield of Micropropagated Sugarcane Following Inoculation by Endophytic Burkholderia vietnamiensis

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