Combined use of eBeam irradiation and the potential probiotic Lactobacillus rhamnosus Vahe for control of foodborne pathogen Klebsiella pneumoniae

Abstract

Purpose The implementation of electron beam radiation coupled with the use of probiotics is one of the newest food processing technologies that may be used to ensure food safety and improve shelf life of food products. The purpose of this study was to evaluate the effect of 50–150-Gy electron beam irradiation on the antimicrobial activity of the putative probiotic strain Lactobacillus rhamnosus Vahe. Methods Low-dose electron beam irradiation of lactobacilli cells was performed using the Advanced Research Electron Accelerator Laboratory’s electron accelerator, and the agar well diffusion method and Verhulst logistic function were used to evaluate the effect of radiation on anti–Klebsiella pneumoniae activity of the cell free supernatant of L. rhamnosus Vahe cells in vitro. Results Our results suggest that 50–150-Gy electron beam irradiation decreases the viability of the investigated lactobacilli, but does not significantly change the probiotic’s activity against K. pneumoniae. Conclusions Results indicate that the combined use of irradiation and L. rhamnosus Vahe might be suggested for non-thermal food sterilizing technologies.

Placebo-resistant gut bacteria: Akkermansia muciniphila spp. and Familial Mediterranean fever disease

“…In the context of FMF disease (Pepoyan et al, 2017;Balayan et al, 2015;Manzano et al, 2023;Touitou and Pepoyan, 2008;Lancieri et al, 2023;Pepoyan et al, 2019b), placebos are employed to evaluate the impacts of probiotics [microorganisms that confer beneficial effects on humans (Pepoyan et al, 2023;Garcıá-Santos et al, 2023;Harutyunyan et al, 2022), animals (Ataya et al, 2023;Balayan et al, 2019;Rodriguez et al, 2017;Wang et al, 2023;Mirzabekyan et al, 2023;S ̌efcováet al, 2023), and plant host metabolism (Rahman et al, 2018;Pepoyan and Chikindas, 2020;Mockevicǐutėet al, 2023)], along with their metabolites, as well as medications in general (Ben-Zvi et al, 2017;Haviv and Hashkes, 2016;Hashkes and Huang, 2015;Hashkes et al, 2014). While placebos have long served as inert controls in clinical trials (Gupta and Verma, 2013;Finniss et al, 2010;Louhiala and Puustinen, 2017), it is essential to recognize that placebo effects are psychobiological phenomena (Pogany, 2017;Hashmi, 2018;Liu, 2022;Schaefer et al, 2023;Shafir et al, 2023) capable of producing effects similar to certain drugs, even when patients are not knowingly given placebos (Bräscher et al, 2022).…”

One Health Probiotics as Biocontrol Agents: One Health Tomato Probiotics

“…The term “probiotics” as “active substances that are essential for a healthy development of life” was described by Werner Kollath in 1953 and by Ferdinand Vergin in 1954 [ 59 , 60 ]. Probiotics are alive bacterial cells that, when administered in adequate amounts, benefit the host’s health [ 61 ] and can have a substantial impact on the functionality of human and animal [ 48 , 62 , 63 , 64 , 65 , 66 , 67 ] organisms, as well as on plants [ 68 ]. Plant probiotics are used to increase resistance to pathogens [ 69 ] and improve yields by reducing or even eliminating chemical fertilizers [ 68 ].…”

“…Moreover, the impact of probiotics on viral and respiratory tract infections has also been described [ 108 ]. It is also possible to “regulate” probiotic antagonistic activities through the use of different technologies [ 62 ]. In food technologies that use tomatoes or their processed products, it is advisable to use plant-specific probiotic strains that have previously been assessed for efficacy and safety in model food systems, as well as in adequate biological test systems [ 109 , 110 ].…”

Fish Probiotics: Cell Surface Properties of Fish Intestinal Lactobacilli and Escherichia coli

“…Therefore, there is an urgent need to develop alternative methods for combating fish pathogens, reducing the accumulation of antibiotic residues in fish meat, and other related environmental problems [ 4 ]. Such methods include phage [ 5 , 6 ] and probiotic therapies [ 7 , 8 , 9 , 10 , 11 , 12 ]. According to the International Scientific Association for Probiotics and Prebiotics, probiotics are live microorganisms that, when administered in adequate amounts, confer a health benefit to the host ( ) (accessed on 17 November 2019) [ 13 ].…”