Combined venomics, venom gland transcriptomics, bioactivities, and antivenomics of two Bothrops jararaca populations from geographic isolated regions within the Brazilian Atlantic rainforest

Machado, Larissa Gonçalves; Plá, Davinia; Sanz, Libia; Jorge, Roberta Jeane Bezerra; Leitão-de-Araújo, Moema; Alves, Maria Lúcia Machado; Alvares, Diego Janisch; Miranda, Joari De; Nowatzki, Jenifer; Morais-Zani, Karen de; Fernandes, Wilson Roberto; Tanaka-Azevedo, Anita Mitico; Fernández, Julián; Zingali, Russolina B.; Gutiérrez, José Marı́a; Corrêa-Netto, Carlos; Calvete, Juan J.

doi:10.1016/j.jprot.2015.04.029

Cited by 120 publications

(91 citation statements)

References 92 publications

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“…Recent studies on the proteomic characterization of venoms are increasingly combining identification data with functional assays of particular components, to gain deeper insights from the medical and biological perspectives [57, 83–85]. The TS is conceptually identical to the ‘lethal neurotoxicity coefficient’ (LNC) defined as the ratio between the average LD 50 and the crotoxin + crotamine relative abundance (% of the total venom proteins) [50].…”

Section: Toxicovenomics: Unmasking the Villains Among The Crowdmentioning

confidence: 99%

Strategies in ‘snake venomics’ aiming at an integrative view of compositional, functional, and immunological characteristics of venoms

Lomonte

Calvete

2017

J Venom Anim Toxins Incl Trop Dis

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129

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This work offers a general overview on the evolving strategies for the proteomic analysis of snake venoms, and discusses how these may be combined through diverse experimental approaches with the goal of achieving a more comprehensive knowledge on the compositional, toxic, and immunological characteristics of venoms. Some recent developments in this field are summarized, highlighting how strategies have evolved from the mere cataloguing of venom components (proteomics/venomics), to a broader exploration of their immunological (antivenomics) and functional (toxicovenomics) characteristics. Altogether, the combination of these complementary strategies is helping to build a wider, more integrative view of the life-threatening protein cocktails produced by venomous snakes, responsible for thousands of deaths every year.

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Section: Toxicovenomics: Unmasking the Villains Among The Crowdmentioning

confidence: 99%

Strategies in ‘snake venomics’ aiming at an integrative view of compositional, functional, and immunological characteristics of venoms

Lomonte

Calvete

2017

J Venom Anim Toxins Incl Trop Dis

Self Cite

129

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“…The dominant families are secreted phospholipases A 2 (PLA2s), snake venom metalloproteinases (SVMP), snake venom serine proteases (SVSP), and three-finger peptides (3FTX), while the secondary families comprise cysteine-rich secretory proteins, Lamino acid oxidases, kunitz peptides, C-type lectins, disintegrins, and natriuretic peptides (Slagboom et al, 2017;Tasoulis and Isbister, 2017;Munawar et al, 2018). Interestingly, snake venom composition varies interspecifically (Fry et al, 2008;Tasoulis and Isbister, 2017), as well as intraspecifically, with many factors influencing this diversity including age (Dias et al, 2013), gender (Menezes et al, 2006;Zelanis et al, 2016), location (Durban et al, 2011;Goncalves-Machado et al, 2016), diet (Barlow et al, 2009), and season (Gubensek et al, 1974). This variability phenomenon underpins toxin diversity and multifunctionality, and is of great importance to be considered in antivenom production and envenomation treatment .…”

Section: Introductionmentioning

confidence: 99%

Multifunctional Toxins in Snake Venoms and Therapeutic Implications: From Pain to Hemorrhage and Necrosis

et al. 2019

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Animal venoms have evolved over millions of years for prey capture and defense from predators and rivals. Snake venoms, in particular, have evolved a wide diversity of peptides and proteins that induce harmful inflammatory and neurotoxic effects including severe pain and paralysis, hemotoxic effects, such as hemorrhage and coagulopathy, and cytotoxic/myotoxic effects, such as inflammation and necrosis. If untreated, many envenomings result in death or severe morbidity in humans and, despite advances in management, snakebite remains a major public health problem, particularly in developing countries. Consequently, the World Health Organization recently recognized snakebite as a neglected tropical disease that affects ∼2.7 million p.a. The major protein classes found in snake venoms are phospholipases, metalloproteases, serine proteases, and three-finger peptides. The mechanisms of action and pharmacological properties of many snake venom toxins have been elucidated, revealing a complex multifunctional cocktail that can act synergistically to rapidly immobilize prey and deter predators. However, despite these advances many snake toxins remain to be structurally and pharmacologically characterized. In this review, the multifunctional features of the peptides and proteins found in snake venoms, as well as their evolutionary histories, are discussed with the view to identifying novel modes of action and improving snakebite treatments.

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“…Venomics is considered an integrative approach, that can combine proteomics, transcriptomics and/or genomics to study venoms [1]. Although the term was initially used to describe the mass spectrometry-based proteomic characterization of venoms [2,3], genomic [4,5] or more commonly venom gland transcriptomic sequencing [6][7][8][9][10][11][12][13][14] have also been used to characterize venom compositions. These molecular approaches provide an overview of venom composition by characterizing the nucleotide sequences of venom toxin-encoding genes (among others) and, in the case of transcriptomics, also provide an estimation of their relative expression in the venom gland.…”

Section: Introductionmentioning

confidence: 99%

Intact protein mass spectrometry reveals intraspecies variations in venom composition of a local population of Vipera kaznakovi in Northeastern Turkey

Petras

Hempel

Göçmen

et al. 2019

Journal of Proteomics

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Combined venomics, venom gland transcriptomics, bioactivities, and antivenomics of two Bothrops jararaca populations from geographic isolated regions within the Brazilian Atlantic rainforest

Cited by 120 publications

References 92 publications

Strategies in ‘snake venomics’ aiming at an integrative view of compositional, functional, and immunological characteristics of venoms

Strategies in ‘snake venomics’ aiming at an integrative view of compositional, functional, and immunological characteristics of venoms

Multifunctional Toxins in Snake Venoms and Therapeutic Implications: From Pain to Hemorrhage and Necrosis

Intact protein mass spectrometry reveals intraspecies variations in venom composition of a local population of Vipera kaznakovi in Northeastern Turkey

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