Combining abilities of in vitro traits in wheat (Triticum aestivum L.) immature embryo cultures

Barakat, M. N.

doi:10.1007/bf00022161

Cited by 9 publications

(5 citation statements)

References 19 publications

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“…This suggested that both additive and non-additive genetic variance may contribute to the genetic expression of immature embryo culture traits. These results are supported by other findings by Barakat (1994) and Abd El-Maksoud (2003).…”

Section: Resultes and Discussionsupporting

confidence: 92%

NATURE OF GENE ACTION FOR IMMATURE EMBRYO CULTURE ABILITY IN WHEAT (Triticum aestivum L(

Habiba

Maksud

Kash

et al. 2012

Journal of Agricultural Chemistry and Biotechnology

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The objective of this study was to determine the genetic parameters involved in immature embryo culture ability in wheat (Triticum aestivum L). For this purpose, five lines and two cultivated varieties were used. These lines were: C.B243, C.B245, C.B249, C.B251 and C.B253. While, the two testers were Gemmieza 9 and Gemmieza 10. The results showed the presence of highly significant differences among genotypes for all in vitro traits. This indicated that embryogenic calli induction and its regeneration were affected by the genotypes of the immature embryo donor plants. The line C.B 251 was the most responsive for both embryogenic calli ratio and regenerable calli ratio, while the line C.B243 was the best combiner among other lines in the case of callus fresh weight. The beast combination for all traits was the cross C.B 251× Gemmieza 9. Furthermore, the results revealed that both additive and nonadditive genetic variances played an important role in the inheritance of immature embryo culture traits. However, the magnitude of non-additive genetic variance was larger than their corresponding estimates of additive genetic variances for callus fresh weight and regenerable calli ratio. This finding was emphasized by the dominance degree value which exceeded unity with respect to these traits. Therefore, it could be concluded that using F1 hybrids for immature embryo culture improvement the ability to induce high ratio of plantlets.

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Section: Resultes and Discussionsupporting

confidence: 92%

NATURE OF GENE ACTION FOR IMMATURE EMBRYO CULTURE ABILITY IN WHEAT (Triticum aestivum L(

Habiba

Maksud

Kash

et al. 2012

Journal of Agricultural Chemistry and Biotechnology

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“…Therefore, shoot formation ability in immature embryo cultures of wheat may be under different genetic control. Recently, Barakat (1994) reported that dominance or dominant types of epistasis may play a role in the immature embryo culture response to in vitro culture traits.…”

Section: Generation Meansmentioning

confidence: 99%

“…The genetic aspects of plant regeneration are generally considered important, but have been less widely studied. Examination of a dialled cross among eight wheat cultivars has provided evidence that both additive and non-additive variations are operating to control the in vitro culture traits, but non-additive gene action predominates (Barakat, 1994).…”

Section: Introductionmentioning

confidence: 99%

Estimation of genetic parameters for in vitro traits in wheat immature embryo cultures involving high X low regeneration capacity genotypes

Barakat

1996

Euphytica

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Estimates of gene actions were obtained for five in vitro traits of immature wheat (Triticum aestivum L.) embryo cultures from a cross of two wheat cultivars and the resulting reciprocal, Fl, F2 and backcross populations. The contribution of additive gene effects to in vitro traits was not as important as the dominance gene effects. Epistatic gene effects were relatively more important than either additive or dominance gene effects. Of the individual types of digenic epistatic effects, the dominance x dominance estimates were relatively larger in magnitude for all in vitro culture traits measured. The maternal effect played a minor role in the inheritance of the in vitro studied traits since the difference among the reciprocal values was not significant. It is shown from the generation mean method that epistasis played a major role in the inheritance of most of the traits under study, The negative values of additive and dominance genetic variance were estimates of zero. Heritability estimates, in broad sense, were relatively high for the in vitro studied traits. In some cases, heritability estimates in broad and narrow senses are almost equal since the estimation of dominance genetic variance led to negative values. According to the results of the gene effects, dominance and epistasis were important for the shoot formation trait. Selection would be effective among the isolated genotypes on individual basis.

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“…A main part of biotechnology application that mediates crops genetic manipulation and serves in plant breeding is regeneration of plants from cells and plant parts via tissue culture. Haplodization, somaclonal variation, cell suspension culture, protoplasts and DNA delivery techniques remain the key approaches of tissue culture to regenerate plant breeding materials (Barakat and Cocking, 1989, Barakat, 1994, Henry et al, 1994Barakat, et al 2017). The expected outcomes of these technologies are increased genetic variation in addition to saving time.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Egyptian Maize Hybrids for Their Capability of Plant Regeneration using Immature Embryo Culture

Awad-Allah

Kuku

Milad

et al. 2021

Alexandria Science Exchange Journal

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The aim of this research was to determine the regeneration capability of five maize hybrids (Syngenta hybrid, Three-way hybrid (324), Single cross hybrid P344 (Pioneer), Single cross hybrid 30N 11 (Pioneer) and Threeway hybrid (321)) using immature embryo explants with four medium protocols. The analysis of variance for in vitro culture traits of explant response indicated that callus induction, callus weight and embryogenic callus significantly responded to maize genotypes, medium protocols and their interaction. Also differences among maize hybrids were highly significant for shoot formation (%), while they were not significant for root formation (%). The four medium protocols indicated highly significant differences for root formation (%) and shoot formation (%). Three-way hybrid (324) and Single cross hybrid 30 N11(Pioneer) had the highest induction frequency of shoot formation on medium protocol D. Both maize hybrids on medium protocol D could be successfully utilized for the in vitro selection studies for maize improvement.

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Combining abilities of in vitro traits in wheat (Triticum aestivum L.) immature embryo cultures

Cited by 9 publications

References 19 publications

NATURE OF GENE ACTION FOR IMMATURE EMBRYO CULTURE ABILITY IN WHEAT (Triticum aestivum L(

NATURE OF GENE ACTION FOR IMMATURE EMBRYO CULTURE ABILITY IN WHEAT (Triticum aestivum L(

Estimation of genetic parameters for in vitro traits in wheat immature embryo cultures involving high X low regeneration capacity genotypes

Assessment of Egyptian Maize Hybrids for Their Capability of Plant Regeneration using Immature Embryo Culture

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