Combining ability between lines of Cucumis sativus L. and Cucumis sativus var. hardwickii (R.) Alef

Kupper, R. S.; Staub, Jack E.

doi:10.1007/bf00023522

Cited by 27 publications

(15 citation statements)

References 23 publications

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“…sativus types (Kupper and Staub, 1998). The genetic diversity depicted by isozymic variation (Figure 2, panels a and b) agrees with differences previously observed intervarietal morphological variation (Kupper and Staub, 1988).…”

Section: Resultssupporting

confidence: 79%

Genetic diversity in cucumber (Cucumis sativus L.): I. A reevaluation of the U.S. germplasm collection

Meglič

Serquén

Staub

1996

Genet Resour Crop Evol

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Genetic variation within the U.S. cucumber collection (Cucumis sativus var. sativus L. and var. hardwickii (Royle)Alef.) was assessed by examing the variation at 21 polymorphic isozyme loci and comparing the results of this investigation with a similar previous analysis of 14 loci. About 29% (15 of 51) of the enzyme systems examined in an initial survey were polymorphic. Seven loci (Ak-2, Ak-3, Fdp-1, Fdp-2, Mpi-1, Pep-gl and Skdh) which were not previously used to estimate genetic diversity, were assessed. On average, 1_4 loci were polymorphic per enzyme system and 2.2 alleles were present per polymorphic locus. The frequency of polymorphisms was relatively low for Fdp-l(2) (0.01), Mpi-l(1) (0.03), and Skdh(1) (0.02). Principal component and cluster analyses of allelic variation at polymorphic loci separated a diverse array of 757 cucumber accessions from the U.S. National Plant Germplasm Systesm's (NPGS) collection into distinct groups by country (45 nations examined). All accessions of C. s. var. sativus were isozymically distinct from C. s. var. hardwickii, which were themselves dissimilar from each other. Data suggest that C. s. var. hardwickii is not a feral derivative of extant C. s. var. sativus populations. The allelic profile of C. s. var. sativus accessions originating from Burma, Thailand, Indonesia, Hong Kong, Zimbabwe and Ethiopia were distinct from the other accessions examined. Allelic fixation has occurred at Pgd-2 in accessions from Burma, and atAk-2 in accessions from Zimbabwe and Ethiopia. Some of the countries examined that were in close geographic proximity (e.g., Thailand, Indonesia and Hong Kong) contained accessions with similar isozyme profiles. Accessions are fixed for certain alleles [e.g., Gr (1) (100%), Fdp-l(1) (100%) and Mpi-2(2) (50%) for accessions from Thailand, Indonesia and Hong Kong]. Grouping countries by continent or sub-continent (i.e., North and South American, China, Eastern Europe, Western Europe) and by numbers of accessions examined (i.e., India/Burma, Iran, Japan, Turkey, and remaining accessions) was used to identify accessions with unique allozymic

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Section: Resultssupporting

confidence: 79%

Genetic diversity in cucumber (Cucumis sativus L.): I. A reevaluation of the U.S. germplasm collection

Meglič

Serquén

Staub

1996

Genet Resour Crop Evol

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“…sativus and var. hardwickii (Kupper and Staub 1988) have been exploited to create high-yielding, multiple disease-resistant lines (Staub et al 1992). The differences observed in the marker-selected backcross progeny described herein portend their potential value in broadening the narrow genetic base of cucumber (3-12% polymorphism; Dijkhuizen et al 1996;Horejsi and Staub 1999).…”

Section: Genetic Diversitymentioning

confidence: 99%

“…Genetic diversity in C. sativus is relatively low (3-8%) when compared with other Cucumis species (10-25%) (Kupper and Staub 1988;Horejsi and Staub 1999;Luan et al 2008). In fact, the genetic diversity of elite cucumber lines is extremely low (1-3%) (Dijkhuizen et al 1996;Staub et al 2002b;Behera et al 2010), highlighting the need for continued introgression of exotic germplasm to ensure broad-based improvement of cucumber.…”

mentioning

confidence: 96%

Marker-assisted backcross selection in an interspecific Cucumis population broadens the genetic base of cucumber (Cucumis sativus L.)

et al. 2010

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Cucumber (Cucumis sativus L.) is a major cucurbit vegetable species whose genetic base has been drastically reduced during its domestication. The crop's narrow genetic base (3-12% DNA polymorphism) has resulted from the use of limited genetic material and intense selection during plant improvement. Recently, however, interspecific hybridization has been successful in Cucumis via mating of C. hystrix Chakr. and C. sativus, which resulted in the amphidiploid C. hytivus. We report herein a marker-assisted strategy for increasing genetic diversity in cucumber through introgression backcrossing employing C. hytivus. The comparatively late-flowering but high-yielding, indeterminate, monoecious line WI 7012A (P 1 ; donor parent) derived from a C. hytivus 9 C. sativus-derived line (long-fruited Chinese C. sativus cv. Beijingjietou) was initially crossed to the determinate, gynoecious C. sativus line WI 7023A (P 2 ; recurrent parent 1), and then advanced backcross generation progeny (BC 2 ) were crossed with the gynoecious indeterminate line WI 9-6A (P 3 ; recurrent parent 2). More specifically, a single F 1 individual (P 1 9 P 2 ) was backcrossed to P 2 , and then BC progeny were crossed to P 2 and P 3 , where markerassisted selection (MAS) for genetic diversity (8 mapped and 16 unmapped markers; designated Sel) or no selection (designated NSel) was applied to produce BC 3 P 2 (Sel) and BC 3 P 3 (Sel), and BC 2 P 2 (NSel) and BC 2 P 2 S 1 (NSel) progeny. Relative vegetative growth, number of lateral branches (LB), days to flowering (DF), yield (fruit number), and fruit quality [as measured by length:diameter (L:D) and endocarp:total diameter (E:T) ratios] were assessed in parents and cross-progeny. DF varied from *20 (BC 3 P 2 Sel) to *25 days (BC 2 P 3 Sel) among the populations examined, where progeny derived from P 2 possessed the shortest DF. Differences in cumulative yield among the populations over six harvests were detected, varying from *8 fruits per plant in BC 3 P 2 (Sel) to *39 fruits per plant in BC 2 P 3 (Sel). Although the vigorous vegetative growth of line P 1 was observed in its backcross progeny, highly heterozygous and polymorphic backcross progeny derived

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“…The genetic variation in cultivated cucumber (C. sativus L.) is relatively limited (Kupper and Staub 1988). Resistance to several agriculturally important diseases and pests has been located in wild Cucumis species, including powdery mildew caused by Sphaerotheca fuliginea (Schlechtend.…”

Section: Introductionmentioning

confidence: 99%

Cucumis monosomic alien addition lines: morphological, cytological, and genotypic analyses

et al. 2003

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Cucumis hystrix Chakr. (HH, 2n=24), a wild relative of the cultivated cucumber, possesses several potentially valuable disease-resistance and abiotic stress-tolerance traits for cucumber ( C. sativus L., CC, 2n=14) improvement. Numerous attempts have been made to transfer desirable traits since the successful interspecific hybridization between C. hystrix and C. sativus, one of which resulted in the production of an allotriploid (HCC, 2n=26: one genome of C. hystrix and two of C. sativus). When this genotype was treated with colchicine to induce polyploidy, two monosomic alien addition lines (MAALs) (plant nos. 87 and 517: 14 CC+1 H, 2n=15) were recovered among 252 viable plants. Each of these plants was morphologically distinct from allotriploids and cultivated cucumbers. Cytogenetic and molecular marker analyses were performed to confirm the genetic constitution and further characterize these two MAALs. Chromosome counts made from at least 30 meristematic cells from each plant confirmed 15 nuclear chromosomes. In pollen mother cells of plant nos. 87 and 517, seven bivalents and one univalent were observed at diakinesis and metaphase I; the frequency of trivalent formation was low (about 4-5%). At anaphase I and II, stochastic and asymmetric division led to the formation of two gamete classes: n=7 and n=8; however, pollen fertility was relatively high. Pollen stainability in plant no. 87 was 86.7% and in plant no. 517 was 93.2%. Random amplified polymorphic DNA analysis was performed using 100 random 10-base primers. Genotypes obtained with eight primers (A-9, A-11, AH-13, AI-19, AJ-18, AJ-20, E-19, and N-20) showed a band common to the two MAAL plants and C. hystrix that was absent in C. sativus, confirming that the alien chromosomes present in the MAALs were derived from C. hystrix. Morphological differences and differences in banding patterns were also observed between plant nos. 87 and 517 after amplification with primers AI-5, AJ-13, N-12, and N-20, suggesting that these plants may contain different C. hystrix chromosomes.

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Combining ability between lines of Cucumis sativus L. and Cucumis sativus var. hardwickii (R.) Alef

Cited by 27 publications

References 23 publications

Genetic diversity in cucumber (Cucumis sativus L.): I. A reevaluation of the U.S. germplasm collection

Genetic diversity in cucumber (Cucumis sativus L.): I. A reevaluation of the U.S. germplasm collection

Marker-assisted backcross selection in an interspecific Cucumis population broadens the genetic base of cucumber (Cucumis sativus L.)

Cucumis monosomic alien addition lines: morphological, cytological, and genotypic analyses

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