Combining AFM, XPS and chemical hydrolysis to understand the complexity and dynamics of C. vulgaris cell wall composition and architecture

Demir-Yilmaz, Irem; Schiavone, Marion; Esvan, Jérôme; Guiraud, Pascal; Formosa-Dague, Cécile

doi:10.1016/j.algal.2023.103102

Cited by 7 publications

(5 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result agrees with the reported composition of C. vulgaris cell-wall which hosts many polysaccharidic compounds (and therewith carbo/hydroxyl groups). 45,46 Fig. 1b–d further show that | μ | decreases with increasing c NaNO 3 as a result of screening of cell charges by electrolyte ions.…”

Section: Resultsmentioning

confidence: 80%

“…C. vulgaris are unicellular eukaryotic and photosynthetic microorganisms possessing a cell membrane formed by a double lipidic layer surrounded by a cell wall (without appendages) whose dimension and density increase during growth. 44,45 The cell wall of C. vulgaris is mostly composed of (poly)saccharides, with the additional presence of proteins and lipids, 46 and there are few indications in literature about the nature of the charge-carrying components, about their surface concentration and distribution at the algal interface formed with the outer aqueous medium. 16,46 In addition, as evidenced by recent work, 35 microalgae can be viewed as soft particles, i.e.…”

Section: Resultsmentioning

confidence: 99%

“…44,45 The cell wall of C. vulgaris is mostly composed of (poly)saccharides, with the additional presence of proteins and lipids, 46 and there are few indications in literature about the nature of the charge-carrying components, about their surface concentration and distribution at the algal interface formed with the outer aqueous medium. 16,46 In addition, as evidenced by recent work, 35 microalgae can be viewed as soft particles, i.e. particles permeable to electrolyte ions and/or electroosmotic flow 32 (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…73 It cannot be excluded that such a distribution pattern of hydrophobic compounds is also related to a specific repartition of lipids within the C. vulgaris cell wall/membrane. 46,74,75 At this stage, the above assumptions are obviously largely speculative, and their validation requires additional analysis that goes beyond the scope of this work.…”

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

Physicochemical surface properties of Chlorella vulgaris: a multiscale assessment, from electrokinetic and proton uptake descriptors to intermolecular adhesion forces

Lesniewska,

Duval,

Caillet

et al. 2024

Nanoscale

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Physicochemical surface properties of Chlorella vulgaris: a multiscale assessment, from electrokinetic and proton uptake descriptors to intermolecular adhesion forces

Lesniewska,

Duval,

Caillet

et al. 2024

Nanoscale

View full text Add to dashboard Cite

show abstract

“…vulgaris CCAP strain for which the average Ym was of 981.6 ± 554.4 kPa at the same pH of 8, this value is significantly higher, meaning that C. vulgaris strain F010102-A cells are thus more rigid, which could be due to a different cell wall organization or a different cell wall composition …”

Section: Resultsmentioning

confidence: 95%

The Biophysical Properties of Microalgal Cell Surfaces Govern Their Interactions with an Amphiphilic Chitosan Derivative Used for Flocculation and Flotation

Demir-Yilmaz,

Pappa,

Lama

et al. 2024

ACS Appl. Bio Mater.

Self Cite

View full text Add to dashboard Cite

Microalgae show great promise for producing valuable molecules like biofuels, but their large-scale production faces challenges, with harvesting being particularly expensive due to their low concentration in water, necessitating extensive treatment. While methods such as centrifugation and filtration have been proposed, their efficiency and cost-effectiveness are limited. Flotation, involving air-bubbles lifting microalgae to the surface, offers a viable alternative, yet the repulsive interaction between bubbles and cells can hinder its effectiveness. Previous research from our group proposed using an amphiphilic chitosan derivative, polyoctyl chitosan (PO-chitosan), to functionalize bubbles used in dissolved air flotation (DAF). Molecular-scale studies performed using atomic force microscopy (AFM) revealed that PO-chitosan's efficiency correlates with cell surface properties, particularly hydrophobic ones, raising the question of whether this molecule can in fact be used more generally to harvest different microalgae. Evaluating this, we used a different strain of Chlorella vulgaris and first characterized its surface properties using AFM. Results showed that cells were hydrophilic but could still interact with PO-chitosan on bubble surfaces through a different mechanism based on specific interactions. Although force levels were low, flotation resulted in 84% separation, which could be explained by the presence of AOM (algal organic matter) that also interacts with functionalized bubbles, enhancing the overall separation. Finally, flocculation was also shown to be efficient and pH-independent, demonstrating the potential of PO-chitosan for harvesting microalgae with different cell surface properties and thus for further sustainable large-scale applications.

show abstract

Behavior and surface properties of microalgae indicate environmental changes

Ivošević DeNardis,

Novosel Vlašić,

Mišić Radić

et al. 2023

J Appl Phycol

View full text Add to dashboard Cite

We conducted a microcosm experiment under near-natural conditions to investigate the effects of environmental stressors temperature and salinity on the response of three microalgal species. The response of microalgae to increased temperature and altered salinity is species-specific, with cell barrier complexity playing an important protective role. At decreasing salinity, the green flagellate cells of Dunaliella tertiolecta vibrate around the point and become stiff while their physiological activity is at its highest, which may indicate that organic matter is being released into the medium. At the highest salinity, Tetraselmis suecica showed the highest growth rate along with the hydrophobic behavior of the cells, which is a favorable condition for the formation of aggregates. Under these conditions, the cells could enter the cyst stage, while the content of polar lipids could mediate cell adhesion to the natural interfaces. Cylindrotheca closterium, a diatom with an organosilicate cell wall, also showed the highest physiological activity at decreasing salinity. However, the cells were softest and exhibited hydrophilic behavior, implying that released organic material could alter the surface properties of the diatom. This study provides insight into the behavioral, mechanical, and molecular changes of microalgae under near-natural conditions, which may contribute to a fundamental understanding of how biochemistry and mechanics interact to control the functional behavior of microalgal cells in climatically altered aquatic systems.

show abstract

Combining AFM, XPS and chemical hydrolysis to understand the complexity and dynamics of C. vulgaris cell wall composition and architecture

Cited by 7 publications

References 57 publications

Physicochemical surface properties of Chlorella vulgaris: a multiscale assessment, from electrokinetic and proton uptake descriptors to intermolecular adhesion forces

Physicochemical surface properties of Chlorella vulgaris: a multiscale assessment, from electrokinetic and proton uptake descriptors to intermolecular adhesion forces

The Biophysical Properties of Microalgal Cell Surfaces Govern Their Interactions with an Amphiphilic Chitosan Derivative Used for Flocculation and Flotation

Behavior and surface properties of microalgae indicate environmental changes

Contact Info

Product

Resources

About